A5100646485- HIV Research and Treatment
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- RNA modifications and cancer
- RNA Research and Splicing
- T-cell and B-cell Immunology
- interferon and immune responses
- CRISPR and Genetic Engineering
- RNA regulation and disease
- HIV/AIDS Research and Interventions
- Chromosomal and Genetic Variations
- SARS-CoV-2 and COVID-19 Research
- Liver Disease and Transplantation
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- COVID-19 Clinical Research Studies
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Vitamin C and Antioxidants Research
- Abdominal vascular conditions and treatments
- Ginseng Biological Effects and Applications
- Pregnancy and Medication Impact
- Fetal and Pediatric Neurological Disorders
Sun Yat-sen University
2014-2025
Guangzhou Medical University
2025
Sun Yat-sen Memorial Hospital
2020-2025
Peking University First Hospital
2024
Peking University
2024
Shandong Academy of Agricultural Sciences
2022
Ministry of Agriculture and Rural Affairs
2022
Nanjing Agricultural University
2021
Second Military Medical University
2021
Changhai Hospital
2021
PIWI interacting RNAs (piRNAs) are highly expressed in germline cells and involved maintaining genome integrity by silencing transposons. These also DNA/histone methylation gene expression regulation somatic of invertebrates. The functions piRNAs vertebrates, however, remain elusive. We found that snoRNA-derived C (C′)/D′ (D)-box conserved abundant human CD4 primary T-lymphocytes. piRNA (piR30840) significantly downregulated interleukin-4 (IL-4) via sequence complementarity binding to...
Abstract The discovery of PIWI-interacting RNAs (piRNAs) revealed the complexity RNA world. Although piRNAs were first deemed to be germline specific, substantial evidence shows their various roles in somatic cells; however, function highly differentiated immune cells remains elusive. In this study, by initially screening with a small deep-sequencing analysis, we found that piRNA, tRNA-Glu–derived piRNA [td-piR(Glu)], was expressed much more abundantly human monocytes than dendritic cells....
Despite the advent of combined antiretroviral therapy (cART), persistence viral reservoirs remains a major barrier to curing human immunodeficiency virus type 1 (HIV-1) infection. Recently, shock and kill strategy, by which such are eradicated following reactivation latent HIV-1 latency-reversing agents (LRAs), has been extensively practiced. It is important reestablish virus-specific reliable immune surveillance eradicate reactivated virus-harboring cells. In this report, we attempted reach...
Abstract Maternal immune system tolerance to the semiallogeneic fetus is essential for a successful pregnancy; however, mechanisms underlying this immunotolerance have not been fully elucidated. Here, we demonstrate that myeloid-derived suppressor cells play an important role in maintaining feto-maternal tolerance. A significant expansion of granulocytic was observed multiple organs and decidual tissues from pregnant mice. Pregnancy-derived suppressed T cell responses reactive oxygen...
Abstract Hepatocellular carcinoma (HCC) causes high morbidity and mortality due to a lack of adequate treatments. Cancer treatments have benefited from nanotechnology approaches that integrate multimodal synergistic therapies. A synergistic, minimally invasive strategy interventional photodynamic therapy (IPDT) chemotherapy for HCC treatment through percutaneous transperitoneal puncture is disclosed based on photosensitive porphyrinic galactose‐modified metal‐organic frameworks (PCN‐224)...
Abstract Cytoskeleton is extensively recruited by flaviviruses for their infection. In this study, we uncovered an essential role of a nuclear membrane protein, SAD1/UNC84 domain protein 2 (SUN2) linking cytoskeleton and nucleoskeleton in the flavivirus replication. CRISPR/Cas9-mediated knockout SUN2, but not SUN1, significantly reduces replication Zika virus (ZIKV), dengue (DENV), Japanese encephalitis (JEV). contrast, SUN2 does affect infection non-flaviviridae RNA viruses. All three...
HIV-1 Rev plays an important role in the late phase of replication, which facilitates export unspliced viral mRNAs from nucleus to cytoplasm infected cells. Recent studies have shown that DDX1 and DDX3 are co-factors for transcripts. In this report, we demonstrated DDX5 (p68), is a multifunctional DEAD-box RNA helicase, functions as new cellular co-factor Rev. We found affects function through Rev-RRE axis subsequently enhances replication. Confocal microscopy co-immunoprecipitation analysis...
The apolipoprotein-B-mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G or A3G) is a potent restrictive factor for human immunodeficiency virus type 1 (HIV-1) and many other retroviruses. It belongs to the cytidine deaminase family. Recent studies have shown that A3G significantly inhibits microRNA (miRNA)-mediated repression of translation. However, mechanism underlying this action must be clarified. In report, we demonstrated counteracts miRNA-mediated translation by inhibiting...
Infection by severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has posed a threat to global public health. The current study revealed that several inhibitors of protein kinases C (PKCs) possess protective activity against SARS-CoV-2 infection. Four pan-PKC inhibitors, Go 6983, bisindolylmaleimide I, enzastaurin, and sotrastaurin, reduced the replication replicon in both BHK-21 Huh7 cells. A PKCδ-specific inhibitor, rottlerin, was also effective reducing viral PKC acted at...
Abstract The CD4+CD25+FOXP3+ regulatory T cells (Tregs) mediate immunological self-tolerance and suppress various immune responses. FOXP3 is a key transcriptional factor for the generation development of Tregs. Its expression regulated by cytokines including TGF-β, IL-2, IL-10. It important to further identify factors Given that many microRNAs (miRNAs) could specifically interact with core promoter region enhance transcription target genes, we searched any possible miRNA(s) targeting gene....
ABSTRACT The SARS-CoV-2 pandemic and the emergence of novel variants underscore need to understand host-virus interactions identify host factors that restrict viral infection. Here, we perform a genome-wide CRISPR knockout screen restriction for SARS-CoV-2, revealing DAZAP2 as potent antiviral gene. DAZAP2, previously implicated in restriction, is found inhibit entry by blocking virion fusion with both endolysosomal plasma membranes. Additionally, suppresses genomic RNA replication without...
Abstract Purpose: Mutations in the KRAS gene, particularly G12D variant, are prevalent intrahepatic cholangiocarcinoma (iCCA) and correlate with unfavorable patient outcomes. Currently, there is an urgent need for targeted therapeutic strategies against KRASG12D iCCA. This study aims to investigate impact mechanisms of mutation on iCCA progression conduct a preclinical evaluation MRTX1133, non-covalent inhibitor KRASG12D. Methods: We developed spontaneous tumorigenesis mouse models harboring...
Abstract Background and aims A marked egg-induced CD4 + T cell programmed inflammation subsequent hepatic fibrosis characterize the pathogenesis of schistosomiasis. Mesenchymal stem (MSC) has been extensively studied for treatment However, mechanism by which MSCs modulate schistosomiasis not clarified. Furthermore, local inflammatory milieu may greatly influence immunoregulatory properties MSCs, our early experiments demonstrated that Toll-like receptor (TLR)2/TLR4 agonist effected immune...
Trichoderma is an important and widely used plant growth-promoting fungus (PGPF). In this study, stevia residue amended with amino acids hydrolyzed from animal carcasses was for the production of guizhouense NJAU 4742 by solid-state fermentation, then its potential to promote corn growth evaluated in combination chemical fertilizer (CF) or organic (OF). The highest spore number 7×109 CFU g−1 fresh weight obtained under following optimal parameters: material ratio 50% (stevia residue:rice...
Although combined antiretroviral therapy (cART) successfully decreases plasma viremia to undetectable levels, the complete eradication of human immunodeficiency virus type 1 (HIV-1) remains impractical because existence a viral reservoir, mainly in resting memory CD4+ T cells. Various cytokines, protein kinase C activators, and histone deacetylase inhibitors (HDACi) have been used as latency-reversing agents (LRAs), but their unacceptable side effects or low efficiencies limit clinical use....
Interleukin-7 (IL-7) has been used as an immunoregulatory and latency-reversing agent in human immunodeficiency virus type 1 (HIV-1) infection. Although IL-7 can restore circulating CD4+ T cell counts HIV-1-infected patients, the anti-apoptotic proliferative effects of appear to benefit survival expansion HIV-1-latently infected memory lymphocytes. shown elevate CD95 on cells individuals prime lymphocytes CD95-mediated or apoptotic signals. Here we observed that through increasing...
Long non-coding RNAs (lncRNAs) represent a new group of host factors involved in viral infection. Current study identified an intergenic lncRNA, LINC08148, as proviral factor Zika virus (ZIKV) and Dengue 2 (DENV2). Knockout (KO) or silencing LINC08148 decreases the replication ZIKV DENV2. mainly acts at endocytosis step but later stage RNA-seq analysis reveals that knockout downregulates transcription levels five endocytosis-related genes including
Zika virus (ZIKV) is an emerging mosquito-borne flavivirus which has become a global epidemic threat due to its rapid spread and association with serious consequences of infection, including neonatal microcephaly. Inositol-requiring enzyme 1α (IRE1α) endoplasmic reticulum (ER)-related transmembrane protein that mediates unfolded response (UPR) pathway been indicated play important role in replication. However, the mechanism how IRE1α affects ZIKV replication remains unknown. In this study,...