A5022251838- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Renal and related cancers
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Parasitic infections in humans and animals
- Parasite Biology and Host Interactions
- Epigenetics and DNA Methylation
- Yeasts and Rust Fungi Studies
- Testicular diseases and treatments
- Genomics and Chromatin Dynamics
- Parasitic Infections and Diagnostics
- Urological Disorders and Treatments
- Retinoids in leukemia and cellular processes
- Birth, Development, and Health
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Fermentation and Sensory Analysis
- Reproductive System and Pregnancy
- Helminth infection and control
- Cancer-related molecular mechanisms research
- Congenital Anomalies and Fetal Surgery
The University of Queensland
2016-2025
QIMR Berghofer Medical Research Institute
1991-2011
Australian Research Council
2006-2011
Victorian Clinical Genetics Services
2011
John Hunter Hospital
2011
Peter MacCallum Cancer Centre
2011
South Australia Pathology
2011
Columbia University
2011
Western University
1998-2008
Osaka University
2006
Germ cells in the mouse embryo can develop as oocytes or spermatogonia, depending on molecular cues that have not been identified. We found retinoic acid, produced by mesonephroi of both sexes, causes germ ovary to enter meiosis and initiate oogenesis. Meiosis is retarded fetal testis action retinoid-degrading enzyme CYP26B1, ultimately leading spermatogenesis. In testes Cyp26b1-knockout embryos, precociously, if a normal ovary. Thus, precise regulation retinoid levels during gonad...
Disorders of sex development (DSDs) are conditions affecting the gonads or genitalia. Variants in two key genes, SRY and its target SOX9, an established cause 46,XY DSD, but genetic basis many DSDs remains unknown. SRY-mediated SOX9 upregulation early gonad is crucial for testis development, yet regulatory elements underlying this have not been identified humans. Here, we four DSD patients with overlapping duplications deletions upstream SOX9. Bioinformatic analysis three putative enhancers...
Girls and young women who require ovariectomy or cancer therapy may consider having their own eggs, embryos ovarian tissue stored (cryopreserved) for future use. Ovarian is simple to collect contains large numbers of germ cells. Transplantation fresh frozen- thawed in healthy sheep mice has resulted normal live young. Similar techniques be effective the human but it unclear whether cryopreservation grafting suitable ovaries from individuals with infections. If cells were present an ovary at...
Recent studies on the epidemiologic pattern of taeniasis in Southeast Asia have indicated existence a third form human Taenia, distinguishable from Taenia saginata and T. solium. Originally termed Taiwan first described Taiwanese aboriginals, this newly recognized taeniid is now generally referred to as Asian since it has been recorded number other countries. Here we used genetic yardstick approach determine whether should most appropriately be considered new, distinct species or subspecies,...
SUMMARY Three nucleotide data sets, two mitochondrial (COI and ND1) one nuclear (ribosomal ITS1), have been investigated in order to resolve relationships among species strains of the genus Echinococcus . The some unusual properties that heteroplasmy was detected strain E. granulosus , more than class ITS1 sequence variant can occur a single isolate. failed support hypothesis as it is currently viewed, valid species. Rather, seem comprise at least three evolutionarily diverse groups, sheep...
Abstract Background Meiosis in higher vertebrates shows a dramatic sexual dimorphism: germ cells enter meiosis and arrest at prophase I during embryogenesis females, whereas males they mitotic only after birth. Here we report the molecular analysis of onset chicken model provide evidence for conserved regulation by retinoic acid. Results embryo is initiated late (day 15.5), relative to gonadal sex differentiation (from day 6). Meiotic are first detectable female gonads from 15.5, correlating...
A successful mitosis-to-meiosis transition in germ cells is essential for fertility sexually reproducing organisms. In mice and humans, it has been established that expression of STRA8 crucial meiotic onset both sexes. Here, we show BMP signalling also essential, not induction but correct progression female mouse fetal cells. Largely agreement with evidence from primordial cell-like (PGCLCs) vitro, cell-specific deletion receptor 1A (BMPR1A; ALK3) caused aberrant retention pluripotency...
SUMMARY We have used a number of molecular genetic approaches to characterize the cervid strain (‘northern form’) Echinococcus granulosus. PCR–RFLP analysis nuclear ITS1 region rDNA repeat can readily distinguish form from other strains E. granulosus . The complexity RFLP patterns obtained suggests that distinct types are present in this which may represent an inter-strain hybrid. Mitochondrial CO1 sequence genotype was ambiguous at 18 positions and closely resembles cluster previously...
Germ cells, the embryonic precursors of sperm or oocytes, respond to molecular cues that regulate their sex-specific development in fetal gonads. In males particular, balance between continued proliferation and cell fate commitment is crucial: defects result insufficient spermatogonial stem cells for fertility, but escape from prolonged pluripotency can cause testicular germ tumors. However, factors this remain unidentified. Here, we show signaling by TGFβ morphogen Nodal its co-receptor...
Sex determination in fetal germ cells depends on a balance between exposure to retinoic acid (RA) and the degradation of RA achieved by testis-specific expression catabolic cytochrome P450 enzyme, CYP26B1. Therefore, identification factors regulating Cyp26b1 gene is an important goal reproductive biology. We used situ hybridization demonstrate that transcription factor genes steroidogenic factor-1 (Sf1) Sry-related HMG box 9 (Sox9) are coexpressed Sertoli cells, whereas Sf1 Leydig mouse...