Girls and young women who require ovariectomy or cancer therapy may consider having their own eggs, embryos ovarian tissue stored (cryopreserved) for future use. Ovarian is simple to collect contains large numbers of germ cells. Transplantation fresh frozen- thawed in healthy sheep mice has resulted normal live young. Similar techniques be effective the human but it unclear whether cryopreservation grafting suitable ovaries from individuals with infections. If cells were present an ovary at...

Sixteen-day-old fetal mouse ovaries were slowly frozen in 1.5 mol dimethylsulfoxide ml−1 and subjected to one of two thawing procedures – fast thaw or slow thaw. Fresh frozen–thawed transplanted orthotopically (to the bursal cavity) either bilaterally unilaterally ovariectomized adult female recipients. also heterotopically (under kidney capsule) intact, females. Transplantation recipients resulted restoration cyclic activity within 20.5 ± 4.7 (mean sem) days 23.4 0.8 orthotopic heterotopic...

Double packaging, in which an inner straw containing the specimen is inserted into outer, larger (here termed `straw-in-straw') to prevent from coming direct contact with liquid nitrogen provides a simple strategy for reducing or eliminating potential contamination risk associated storage nitrogen. This approach has past been used conjunction cryopreservation by slow cooling, but not previously tested use throughout entire rapid cooling and warming procedure. study determined whether keeping...

Abstract Cumulus‐intact and ‐denuded unfertilized oocytes from two mouse strains were exposed to 1.5 m ethanol (EtOH) or cryoproteclant solutions, M propanediol (PROH) dimethylsulfoxide (DMSO), for 4.5 min at 27°C, the proportion of activating degenerating studied. Exposure DMSO did not significantly increase activation above that DMSO. Treatment in PROH resulted up 87% viable oocytes. This was higher ( P <01) than control comparable rate after treatment with EtOH (59–96% activation). In...

Summary. This paper investigates the effect of straw handling on viability 2-cell mouse embryos rapidly frozen in dimethyl sulphoxide (DMSO) solutions. During brief (3 min) equilibration step, straws were either rotated periodically to keep suspension, or kept still allow settle onto inner surface straw. The effects these movements tested with cryoprotectant solutions containing 1·5, 3·0 4·5 m-DMSO. Rapidly cooled m-DMSO vitrify throughout cooling, but ice forms warming. survival and...

Abstract Background Gastroduodenal obstruction due to malignancy can be difficult palliate. Self-expanding metal stents (SEMS) are gaining acceptance as an effective alternative surgical bypass. Methods Patients not suitable for bypass, with complete gastric outlet a result of malignancy, were offered palliation SEMS from November 2004 December 2008. The procedure was performed under fluoroscopic guidance and conscious sedation. Data collected prospectively. Results Seventy patients...

Mouse pronuclear and 4-cell embryos were cryopreserved by slow cooling to −33°C in 1.5 M 1, 2-propanediol or ethylene glycol, with without 0.1 sucrose. Straws thawed immersion into a 37°C water bath, immediately after their removal from liquid nitrogen (protocol 1), being held air for 15 2) 30 s 3). Others until the ice melted 4). Embryos which formed blastocysts that hatched attached Petri dish vitro (plated) considered viable. The thawing protocol did not significantly influence viability...

Ovarian tissue xenografting may be applied to increase the population size of rare or endangered animals. However, optimal grafting conditions, such as graft position and recipient hormonal status, are yet established. The present study, using common wombat ovarian tissue, showed that development xenografted antral follicle stage can achieved irrespective position. increased recovery rates survival were evident after under kidney capsule compared with subcutaneous sites. No in was observed...

This study examined the effects of cooling and cryopreservation upon macropod spermatozoa (eastern grey kangaroo, Macropus giganteus red-necked wallaby, rufogriseus). Sperm survival during after freezing to –30˚C or –70˚C in minimum essential medium (MEM) + 5, 10, 20 30% (v/v) glycerol, MEM 10 20% ethylene glycol containing a mixture 7.5% glycerol 10% dimethylsulphoxide was by cryomicroscopy. The MEM/glycerol mixtures permitted better post-thaw sperm recovery than other cryoprotectants....

Developmentally competent oocytes can be collected from xenografted ovarian tissues; however, optimal xenograft conditions need to established for this technique of use in assisted reproduction. In the present study, common wombat tissue was under kidney capsule nude mice clarify role recipient gonadal status and donor age on graft establishment, follicle development oocyte recovery. Eighty-nine per cent all grafts were recovered; these, 78% contained growing follicles. female recipients,...