A5069167729- CRISPR and Genetic Engineering
- Animal Disease Management and Epidemiology
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Biosensors and Analytical Detection
- Muscle Physiology and Disorders
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Nanomaterials and Printing Technologies
- Platelet Disorders and Treatments
- Complement system in diseases
- COVID-19 diagnosis using AI
- Brucella: diagnosis, epidemiology, treatment
- Pluripotent Stem Cells Research
- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 detection and testing
- Fluid Dynamics and Heat Transfer
- interferon and immune responses
- Microbial infections and disease research
- Lipoproteins and Cardiovascular Health
Jilin University
2011-2023
Jilin Medical University
2018
Abstract Genetically modified pigs are increasingly used for biomedical and agricultural applications. The efficient CRISPR/Cas9 gene editing system holds great promise the generation of gene-targeting without selection marker genes. In this study, we aimed to disrupt porcine myostatin ( MSTN ) gene, which functions as a negative regulator muscle growth. transfection efficiency fetal fibroblasts (PFFs) was improved facilitate targeting Cas9/gRNA. We also demonstrated that Cas9/gRNA can...
Classical swine fever (CSF) caused by classical virus (CSFV) is one of the most detrimental diseases, and leads to significant economic losses in industry. Despite efforts many government authorities stamp out disease from national pig populations, remains widespread. Here, antiviral small hairpin RNAs (shRNAs) were selected then inserted at porcine Rosa26 (pRosa26) locus via a CRISPR/Cas9-mediated knock-in strategy. Finally, anti-CSFV transgenic (TG) pigs produced somatic nuclear transfer...
Genetically modified pigs have important roles in agriculture and biomedicine. However, genome-specific knock-in techniques are still their infancy optimal strategies not been extensively investigated. In this study, we performed electroporation to introduce a targeting donor vector (a non-linearized that did contain promoter or selectable marker) into Porcine Foetal Fibroblasts (PFFs) along with CRISPR/Cas9 vector. After optimization, the efficiency of EGFP site-specific could reach up...
Precise genome editing in livestock is of great value for the fundamental investigation disease modeling. However, genetically modified pigs carrying subtle point mutations were still seldom reported despite rapid development programmable endonucleases. Here, we attempt to investigate single-stranded oligonucleotides (ssODN) mediated knockin by introducing two orthologous pathogenic mutations, p.E693G Alzheimer's and p.G2019S Parkinson's disease, into porcine APP LRRK2 loci, respectively....
The fat-1 gene from Caenorhabditis elegans encodes a fatty acid desaturase which was widely studied due to its beneficial function of converting n-6 polyunsaturated acids (n-6PUFAs) n-3 (n-3PUFAs). To date, many transgenic animals have been generated study disease pathogenesis or improve meat quality. However, all them were using random integration method with variable transgene expression levels and the introduction selectable marker genes often raise biosafety concern. this end, we aimed...
Bioinspired hierarchically hairy particles are prepared by using initiator droplets as dynamic templates to achieve a robust superhydrophobic coating.
Contents MicroRNAs (miRNAs) are a class of small, non‐coding RNAs approximately 22 nucleotides in length that regulate gene expression by binding to the 3′‐untranslated regions target mRNAs. It is now clear miRNAs involved many biological processes, including proliferation, differentiation and regulation during early embryonic development. The miRBase 16.0 (2010) shows there 175, 673, 408 1048 annotated for Caenorhabditis elegans , Mus musculus Rattus norvegicus Homo sapiens respectively....
Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous expression. In this study, we proposed an microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine
Abstract Classical swine fever (CSF) caused by classical virus (CSFV) is among the most detrimental diseases, and leads to significant economic losses in industry. Despite efforts many government authorities try stamp out disease from national pig populations, remains widespread. Here, antiviral small hairpin RNAs (shRNAs) were selected then inserted at porcine ROSA26 (p ) locus via a CRISPR/Cas9-mediated knock-in strategy. Finally, anti-CSFV transgenic (TG) pigs produced somatic nuclear...
Infectious diseases caused by pathogenic bacteria (such as sepsis and meningitis) seriously threaten public health; therefore, rapid accurate identification of the target is urgently needed to prevent treat bacterial infections. Although technologies including plate-counting polymerase chain reaction have been established detect bacteria, they are either time-consuming or sophisticated. Herein, a biomimetic octopus-like structure integrating merits multiarm multivalent interaction designed...
Classical swine fever (CSF) is a highly contagious disease that causes devastating economic losses. However, there are few efficacious therapeutic antibodies against the CSF virus (CSFV). Accordingly, we isolated two whole-porcine anti-CSFV neutralizing (NAbs) directly from single B cells sorted using conserved linear epitope of CSFV E2 protein and goat anti-pig IgG. These mAbs, termed HK24 HK44, can bind to by recognizing sites within E2. In addition, these mAbs detect infection with high...
Abstract Recently, there are several routes for COVID-19 vaccine research, yet their weaknesses lie in low efficiency, tolerability, immune adaptability and safety. We describe a new approach to based on engineered human mesenchymal stem cells(hu-MSC), which is like small protein antigen response device, but will be gradually cleared degraded by body’s system among recognization process. The antibody results show that this effective fast. Furthermore, after tests, we obtained an injection of...
The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based methods were recommended for routine detection RNA. Because the time and analytical sensitivity qRT-PCR limits diagnosis SARS-CoV-2, development quick process technology with high remains urgent.We combined isothermal amplification fluorescence develop new auto-recombinase polymerase (RPA)-fluorescence platform that could be used...
The diagnosis of brucellosis largely relies on tiger red plate agglutination test (RBPT). However, it is difficult to distinguish between natural infection antibody positive and vaccination positive, nevertheless, the identification specific Brucella species infection. Here, we analyzed structure main outer membrane proteins (OMPs), OMP25 OMP31 from ovis (B. ovis) melitensis melitensis), which are pathogens sheep brucellosis, found could be used as differential antigens for B. antibody. Then...
Epitope-specific neutralizing antibodies (EsAbs) are of prime importance in the diagnosis and treatment various serious diseases. However, obtaining EsAbs by monoclonal antibody technique involves time-consuming sophisticated multistep procedures, epitopes resulting often not explicit. It is also very challenging to isolate from numerous kinds total immunoglobulins because nonspecific adsorption low separation efficiency. Herein, a magnetic core@multiarm shell-epitope (M@A-E) bioconjugate...
Abstract Classical swine fever (CSF) is a highly contagious disease found worldwide that has caused devastating economic losses. However, there are few efficacious mAbs against the CSF virus (CSFV) can be used for treatment because most CSFV derived from mouse hybridoma cells and these murine have disadvantages of inefficient effector functions elicitations high immunogenicity in vivo . Accordingly, we characterized whole-porcine anti-CSFV neutralizing (NAbs) isolated directly single B...