A5025475158- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Phosphodiesterase function and regulation
- Crystal structures of chemical compounds
- Endoplasmic Reticulum Stress and Disease
- Metal complexes synthesis and properties
- Cholinesterase and Neurodegenerative Diseases
- Gut microbiota and health
- Neurological disorders and treatments
- Radiopharmaceutical Chemistry and Applications
- Viral gastroenteritis research and epidemiology
- Cannabis and Cannabinoid Research
- Ion channel regulation and function
- Heat shock proteins research
- Advanced biosensing and bioanalysis techniques
- Neuroendocrine regulation and behavior
- Pharmacological Receptor Mechanisms and Effects
- Histone Deacetylase Inhibitors Research
Emory University
2022-2025
Harvard University
2021-2024
Massachusetts General Hospital
2021-2024
Shanghai Institute of Organic Chemistry
2016-2024
Chinese Academy of Sciences
2016-2024
University of Chinese Academy of Sciences
2018-2024
Shenyang Agricultural University
2020-2024
Rice Research Institute
2020-2024
Harbin Medical University
2007-2024
Fourth Affiliated Hospital of Harbin Medical University
2016-2024
Significance Posttranslational modifications (PTMs) play an important role in modulating α-syn structure, function, and pathology. Phosphorylation commonly applies to pathological aggregation Lewy bodies is a hallmark of Parkinson’s disease (PD). Here, we obtain homogeneous fibrils formed by semisynthesized with site-specific phosphorylation at Y39. The synthetic fibril recapitulates the neuropathology degradation resistance endogenous pY39 PD. Cryo-EM structure determination reveals fold...
Amyloid aggregation of α-synuclein (α-syn) is closely associated with Parkinson's disease (PD) and other synucleinopathies. Several single amino-acid mutations (e.g. E46K) α-syn have been identified causative to the early onset familial PD. Here, we report cryo-EM structure an fibril formed by N-terminally acetylated E46K mutant (Ac-E46K). The represents a distinct fold α-syn, which demonstrates that mutation breaks electrostatic interactions in wild type (WT) thus triggers rearrangement...
The human cervicovaginal microbiota resides at an interface between the host and environment may affect susceptibility to disease. Puerto Rican women have high HPV infection cervical cancer rates. We hypothesized that population structure of bacterial fungal biota changed with squamous intraepithelial lesions infections. DNA was extracted from cervix, introitus anal sites 62 patients attending high-risk San Juan clinics. 16S rRNA V4 region ITS-2 regions were amplified sequenced using...
The spontaneous self-assembly of multicellular ensembles into living materials with synergistic structure and function remains a considerable challenge in biotechnology synthetic biology. Here, we exploit the aqueous two-phase separation dextran-in-PEG emulsion micro-droplets for capture, spatial organization immobilization algal cells or algal/bacterial cell communities to produce discrete spheroids capable both aerobic (oxygen producing) hypoxic (hydrogen photosynthesis daylight under air....
The spread of pathological α-synuclein (α-syn) is a crucial event in the progression Parkinson's disease (PD). Cell surface receptors such as lymphocyte activation gene 3 (LAG3) and amyloid precursor-like protein 1 (APLP1) can preferentially bind α-syn over monomeric state to initiate cell-to-cell transmission. However, molecular mechanism underlying this selective binding unknown. Here, we perform an array biophysical experiments reveal that LAG3 D1 APLP1 E1 domains commonly use alkaline...
Coacervate-based membraneless organelles with diverse functionality as well the capability of mimicking intracellular physiological environments are attracting researchers' great interest. However, further studies focusing on functionalized membranization coacervate a step toward an advanced membrane-bound protocell still rare. In this study, we develop way to compartmentalize based reconstitution natural cellular wall, which could then serve promising chassis for development protocells...
ABSTRACT Accumulation of aggregated α-synuclein (α-syn) in Lewy bodies is the pathological hallmark Parkinson's disease (PD). Genetic mutations lipid metabolism are causative for a subset patients with Parkinsonism. The role α-syn's interactions its function and aggregation recognized, yet specific lipids involved how issues trigger α-syn neurodegeneration remain unclear. Here, we found that shows preference binding to lysophospholipids (LPLs), particularly targeting lysophosphatidylcholine...
Production of 12-L-hydroxy-5, 8, 10-heptadecatrienoic acid (12-HHT) from platelets and bleeding times were studied in 32 males during acetylsalicylic (ASA) treatment 1 2 weeks after withdrawal. All patients (age 42-77 years) had ASA because angina pectoris. The metabolite 12-HHT is formed the same amount as proaggregatory vasoactive thromboxane A2. Initially daily dose was 75 mg (n = 15), 160 12) or 250-300 5). In all patients, median level increased 40 to 240g/750 x 10(6) (P < 0.001) week...
The abnormal aggregation of α-synuclein (α-Syn) is closely associated with Parkinson's disease. Different post-translational modifications α-Syn have been identified and contribute distinctly in cytotoxicity. Recently, was reported to be N-terminally acetylated cells, yet the functional implication this modification, especially oligomerization, remains unclear. By using a solid-state nanopore system, we found that N-terminal acetylation can significantly decrease oligomerization....
Prior studies have suggested a potential link between nasal microbes and granulomatosis with polyangiitis (GPA; Wegener's), but these relied on culture-dependent methods. This study comprehensively examined the entire community of microbiota (bacteria fungi) in participants GPA compared healthy controls using deep sequencing methods.16S rRNA internal transcribed spacer gene were performed microbial DNA isolated from swabs 60 41 controls. Alpha beta diversity assessed as well relative...
A bio-inspired proteinosome-based material showed programmed mutual interaction with <italic>E. coli</italic> by controlling the temperature and ionic strength.
Abstract Acute flaccid myelitis (AFM) is a polio-like disease that results in paralysis previously healthy persons. Although the definitive cause of AFM remains unconfirmed, enterovirus D68 (EV-D68) suspected based on 2014 data demonstrating an increase cases concomitant with EV-D68 outbreak. We examined prevalence children and molecular evolution for 2009–2018 Philadelphia, Pennsylvania, USA. detected widespread circulation 2009, rare detections 2010 2011, then biennial circulation, only...
Alterations in brain cholesterol homeostasis have been broadly implicated neurological disorders. Notwithstanding the complexity by which biology is governed mammalian brain, excess neuronal primarily eliminated metabolic clearance via cytochrome P450 46A1 (CYP46A1). No methods are currently available for visualizing metabolism living human brain; therefore, a noninvasive technology that quantitatively measures extent of CYP46A1 could affect disease diagnosis and treatment options using...