The spontaneous self-assembly of multicellular ensembles into living materials with synergistic structure and function remains a considerable challenge in biotechnology synthetic biology. Here, we exploit the aqueous two-phase separation dextran-in-PEG emulsion micro-droplets for capture, spatial organization immobilization algal cells or algal/bacterial cell communities to produce discrete spheroids capable both aerobic (oxygen producing) hypoxic (hydrogen photosynthesis daylight under air....

Coacervate-based membraneless organelles with diverse functionality as well the capability of mimicking intracellular physiological environments are attracting researchers' great interest. However, further studies focusing on functionalized membranization coacervate a step toward an advanced membrane-bound protocell still rare. In this study, we develop way to compartmentalize based reconstitution natural cellular wall, which could then serve promising chassis for development protocells...

Abstract The engineering and modulation of living micro-organisms is a key challenge in green bio-manufacturing for the development sustainable carbon-neutral energy technologies. Here, we develop cellular bionic approach which algal cells are interfaced with an ultra-thin shell conductive polymer along calcium carbonate exoskeleton to produce discrete micro-niche capable sustained photosynthetic photosynthetic-independent hydrogen production. surface-augmented induce oxygen depletion,...

Abstract Towards intracellular engineering of living organisms, the development new biocompatible polymerization system applicable for an intrinsically non-natural macromolecules synthesis modulating organism function/behavior is a key step. Herein, we find that tyrosine residues in cofactor-free proteins can be employed to mediate controlled radical under 405 nm light. A proton-coupled electron transfer (PCET) mechanism between excited-state TyrOH* residue and monomer or chain agent...

In this study, the aluminium alloy shell forming process is firstly studied and analysed. After that, three-dimensional modelling software SOLIDWORKS used to analyse model workpieces convex concave dies of each hot extrusion process. Finally, finite element numerical simulation DEFORM-3D carry out analysis on precision shell. Equivalent force strain distributions, along with stroke-load curves, were analyzed under varying conditions by changing friction factor temperature. The results show...

The ultra-wideband (UWB) base station (BS) deployment pattern seriously affects mobile tag positioning accuracy, but the traditional classical methods, such as rectangular and diamond deployment, cannot take into account influence of non-line-of-sight (NLOS) occlusion, which leads to a blind area in positioning. In this paper, we propose new UWB BS optimization method that takes NLOS determines range occlusion by indoor map information, uses locatable points coverage rate whole fitness...

Ordered polycyclization catalyzed by oxidosqualene synthases (OSCs) morph a common linear precursor into structurally complex and diverse triterpene scaffolds with varied bioactivities. We identified three OSCs from Iris tectorum. ItOSC2 is rare multifunctional α-amyrin synthase. Sequence comparisons, site-directed mutagenesis, multiscale simulations revealed that spatially clustered residues, Y531/L256/L258, form an unusual Y-LL triad at the active site, replacing highly conserved W-xY...

Satellite clock offset is an important factor affecting the accuracy of real-time precise point positioning (RT-PPP). Due to missing service (RTS) products provided by International GNSS Service (IGS) or network faults, users may not obtain effective corrections, resulting in unavailability RT-PPP. Considering this issue, improved back propagation (BP) neural optimized heterogeneous comprehensive learning and dynamic multi-swarm particle swarm optimizer (HPSO-BP) proposed for prediction. The...

Inspired by the compartmentalized structure of cells, self-regulating responsive hollow microcapsules are highly desirable for modulation enzymatic reactions. Here, we report a strategy to fabricate gold nanorod embedded proteinosomes covalently grafting nanorods onto surface proteinosomes. The excellent photothermal conversion efficiency and thermal phase transition grafted PNIPAAm allow constructed hybrid show reversible contraction behaviors triggered near-infrared light with molecular...

Abstract Inspired by the unique characteristics of living cells, creation life‐inspired functional ensembles is a rapidly expanding research topic, enabling transformative applications in various disciplines. Herein, we report facile method for fabrication phospholipid and block copolymer hybrid bi‐microcompartments via spontaneous asymmetric assembly at water/tributyrin interface, whereby temperature‐mediated dewetting inner microcompartments allowed exocytosis to occur constructed system....

As a significant space–time infrastructure, the Global Navigation Satellite System (GNSS) provides high-precision positioning, navigation, and timing (PNT) information to users all over world. However, GNSS real-time kinematic (RTK) mobile receiver signal attenuation is obvious in complex environments such as under trees, urban canyons, indoors, among others, it incapable of meeting demand multi-level mass for indoor outdoor seamless positioning applications. The goal this study was address...

A spatially segregative coacervate-in-proteinosome hybrid microcompartment is constructed by co-encapsulation of either positively or negatively charged polyelectrolytes within proteinosomes with enhanced cascade enzymatic reactions, providing a step towards the development artificial eukaryotic cell like microcompartments.

Gold nanoparticle-based microcapsules based on the interfacial assembly significantly enhanced biphasic catalytic reaction rate upon near-infrared light irradiation.

Cell surface engineering is an effective strategy to improve the tolerance of living cells against harsh environments as well endow with various properties and functionalities, which they do not possess inherently. However, research emphasis on single-cell emergence collective functionality still rare. In this study, we encapsulate a single cell TiO2 nanoparticles (Degussa P25, composed anatase rutile crystallites) conductive polymer polypyrrole (PPy). The driving force generated from PPy...

In recent years, low-cost single-frequency GNSS receivers have been widely used in many fields such as mass navigation and deformation monitoring; however, due to the poor signal quality of patch antennae, it is difficult for carrier phase real-time kinematic (RTK) technology fix integer ambiguity. Differential (DGNSS) positioning with pseudorange can effectively meet high robustness reliability requirements submeter meter level accuracy UVA/vehicle/aerospace users. To improve DGNSS complex...

The restart of the receiver will lead to change in non-overlapping frequency inter-system biases (ISB), which make it difficult apply tightly combined RTK method pre-calibrating ISB actual scene. Particle swarm optimization (PSO) algorithm can be used estimate fractional part phase bias (F-ISPB) real time, is not affected by restart. However, standard PSO easily fall into local optimum and cannot accurately value F-ISPB. In this contribution, based on characteristics F-ISPB, we propose an...

GNSS-only attitude determination is difficult to perform well in poor-satellite-tracking environments such as urban areas with high and dense buildings or trees. In addition, it harder resolve integer ambiguity the case of single-frequency single-epoch process mode. this contribution, a low-cost MEMS gyroscope integrated multi-antenna GNSS improve performance determination. A new tightly coupled (TC) model proposed, which uses single filter achieve optimal estimation drift, gyro biases...

Digital light processing (DLP) based stereolithography opens new possibilities for fabricating ceramic accessories with complex structures. The cure depth is a critical criterion analyzing the production efficiency of accessories. As predicted using absorption model, absorbance from photoinitiator and dye has direct effect on depth. In this study, blue-colored powder composed 3 mol% yttria-stabilized tetragonal zirconia polycrystals (3Y-TZP) CoAl2O4 was prepared through ball-milling. single...

Steel truss girder suspension bridges are gradually applied to long-span railway bridges. The fatigue damage correction factor is an important parameter for calculation, however the research on coefficient limited small-span This paper analyzes force characteristics of main members, calculates and studies influences train loading length, annual traffic level coefficient. conclusions as follows: (1) influence line stress members were studied; range under tension or mainly analyzed, ranges...

With the gradual development and modernization of satellite navigation systems, using observation information from multi-GNSS has become one hot-spot issues in recent years. Multi-system loose combinations form double-difference equations within their respective positioning effect is improved. However, interchangeability compatible interoperability between global systems (GNSS) cannot be truly realized. At same time, when number visible satellites decreases abruptly, performance deteriorates...