A5021217126- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- RNA Research and Splicing
- Alzheimer's disease research and treatments
- Crystallography and molecular interactions
- Synthesis and properties of polymers
- Parkinson's Disease Mechanisms and Treatments
- Particle Accelerators and Free-Electron Lasers
- Gyrotron and Vacuum Electronics Research
- Protein Structure and Dynamics
- Epoxy Resin Curing Processes
- Cellular Mechanics and Interactions
- Enzyme Structure and Function
- Particle accelerators and beam dynamics
- Receptor Mechanisms and Signaling
- Advanced Electron Microscopy Techniques and Applications
- Covalent Organic Framework Applications
- Radioactive element chemistry and processing
- Photosynthetic Processes and Mechanisms
- Venomous Animal Envenomation and Studies
- Plant Stress Responses and Tolerance
- RNA modifications and cancer
- Polymer Surface Interaction Studies
- Ion channel regulation and function
- Chemical Synthesis and Characterization
Shanghai Institute of Organic Chemistry
2018-2023
Chinese Academy of Sciences
2009-2023
Westlake University
2023
Changchun Institute of Applied Chemistry
2022-2023
University of Science and Technology of China
2022-2023
Institute of Natural Science
2023
ShanghaiTech University
2020-2022
Southwest University
2014-2022
Jinzhou Medical University
2022
University of Chinese Academy of Sciences
2009-2021
Abstract Subcellular membrane-less organelles consist of proteins with low complexity domains. Many them, such as hnRNPA1, can assemble into both a polydisperse liquid phase and an ordered solid amyloid fibril. The former mirrors biological granule assembly, while the latter is usually associated neurodegenerative disease. Here, we observe reversible formation hnRNPA1 that synchronizes liquid–liquid separation, regulates fluidity mobility liquid-like droplets, facilitates recruitment stress...
Amyloid aggregation of α-synuclein (α-syn) is closely associated with Parkinson's disease (PD) and other synucleinopathies. Several single amino-acid mutations (e.g. E46K) α-syn have been identified causative to the early onset familial PD. Here, we report cryo-EM structure an fibril formed by N-terminally acetylated E46K mutant (Ac-E46K). The represents a distinct fold α-syn, which demonstrates that mutation breaks electrostatic interactions in wild type (WT) thus triggers rearrangement...
Many human neurodegenerative diseases are associated with amyloid fibril formation. Inhibition of formation is importance for therapeutics the related diseases. However, development selective and potent inhibitors remains challenging. Here based on structures A fibrils their amyloid-forming segments, we designed a series peptide using RosettaDesign. We further utilized chemical scaffold to constrain peptides into β-strand conformation, which significantly improves potency against aggregation...
The protein tyrosine phosphatase SHP2 mediates multiple signal transductions in various cellular pathways, controlled by a variety of upstream inputs. dysregulation is causative different types cancers and developmental disorders, making it promising drug target. However, how modulated its regulators remains largely unknown. Here, we use single-molecule fluorescence resonance energy transfer molecular dynamics simulations to investigate this question. We identify partially open, semiactive...
Abstract Microenvironment is critical to the function of cells and organisms. One example provided by biomolecular condensates, whose microenvironment can be vastly different from surrounding cellular environments engage unique biological functions. How microenvironments condensates affect their structure remains unknown. Here, we show that arrangements partitioning biomolecules are dictated differences between micropolarity each subcompartment. Sufficient difference in results layered...
Microcrystal electron diffraction (MicroED) is becoming a powerful tool in determining the crystal structures of biological macromolecules and small organic compounds. However, wide applications this technique are still limited by special requirement for radiation-tolerated movie-mode camera lack automated data collection methods. Herein, we develop stage-camera synchronization scheme to minimize hardware requirements enable use conventional cryo-microscope with single-frame CCD camera,...
In vitro studies have indicated that the maturation-inducing hormone 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP, DHP), probably through nuclear progestin receptor (Pgr), might be involved in proliferation of spermatogonial cells and initiation meiosis several fish species. However, further vivo evidence is required to elucidate role DHP spermatogenesis during sexual differentiation teleosts. this study, we cloned pgr analyzed its expression Nile tilapia ( Oreochromis niloticus ) treated...
Abstract Signal transduction systems enable organisms to monitor their external environments and accordingly adjust the cellular processes. In mast cells, second messenger Ap 4 A binds histidine triad nucleotide-binding protein 1 (HINT1), disrupts its interaction with microphthalmia-associated transcription factor (MITF), eventually activates of genes downstream MITF in response immunostimulation. How HINT1 recognizes is regulated by remain unclear. Here, using eight crystal structures,...
G protein-coupled receptor 12 (GPR12) is an orphan that highly expressed in the thalamus of brain and plays a vital role driving thalamocortical functions short-term memory. GPR12 performs high constitutive activity couples with Gs, increasing intracellular cyclic adenosine monophosphate (cAMP) level when it expressed. However, exploitation for drug development limited since unclear how initiates self-activation signal transduction, whether can be modulated by endogenous or synthetic...
Abstract Polyimide (PI) films with extremely high heat‐resisting and dimensional stability are ideal substrate materials for flexible organic light‐emitting diodes. In this study, three diamines containing rigid bisbenzoxazole structures, 2,2′‐ p ‐phenylenebis(5‐aminobenzoxazole) (phDBOA), m ‐pyridylenebis(5‐aminobenzoxazole), ‐pyridylenebis(5‐aminobenzoxazole) ( ‐PDBOA ), were polymerized 3,3′,4,4′‐biphenyl tetracarboxylic dianhydride pyromellitic (PMDA) separately traditional two‐step...
Detergents are the most frequently applied reagents in membrane protein (MP) studies. The limited diversity of one-head-one-tailed traditional detergents, however, is far from sufficient for structurally distinct MPs. Expansion detergent repertoire has a continuous momentum. In line with speculation that pre-assembly exerts superiority, herein we report first time cross-conjugation two series monomeric detergents constructing two-dimensional library dimeric detergents. Optimum stood out...