A5085315184- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Adenosine and Purinergic Signaling
- Neuroblastoma Research and Treatments
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Bacterial biofilms and quorum sensing
- Water Treatment and Disinfection
- Lipid Membrane Structure and Behavior
- Prenatal Substance Exposure Effects
- Pharmacological Receptor Mechanisms and Effects
- Cannabis and Cannabinoid Research
- Diabetes Treatment and Management
- Marine Toxins and Detection Methods
- Vibrio bacteria research studies
- Cell Adhesion Molecules Research
- Reproductive System and Pregnancy
- Cancer, Hypoxia, and Metabolism
- Ion Transport and Channel Regulation
- Computational Drug Discovery Methods
- Microbial Community Ecology and Physiology
- Neurotransmitter Receptor Influence on Behavior
- Biosensors and Analytical Detection
East China Normal University
2019-2025
ShanghaiTech University
2018-2020
Hebei Medical University
2017
Second Hospital of Hebei Medical University
2017
Stony Brook University
2013-2015
Cell signaling plays an important role in the survival of bacterial colonies. They use small molecules to coordinate gene expression a cell density dependent manner. This process, known as quorum sensing, helps bacteria regulate diverse functions such bioluminescence, biofilm formation and virulence. In Vibrio harveyi, bioluminescent marine bacterium, four parallel quorum-sensing systems have been identified light production. We previously reported that nitric oxide (NO), through H-NOX/HqsK...
The human adenosine A 2B receptor (A R) is a class G protein–coupled that involved in several major physiological and pathological processes throughout the body. R recognizes its ligands NECA with relatively low affinity, but detailed mechanism for ligand recognition signaling still elusive. Here, we present two structures determined by cryo–electron microscopy of bound to agonists BAY60-6583, each coupled an engineered s protein. reveal conserved orthosteric binding pockets subtle...
G protein-coupled receptor 4 (GPR4) belongs to the subfamily of proton-sensing GPCRs (psGPCRs), which detect pH changes in extracellular environment and regulate diverse physiological responses. GPR4 was found be overactivated acidic tumor microenvironment as well inflammation sites, with a triad residues within transmembrane domain identified crucial for proton sensing. However, 3D structure remains unknown, roles other conserved psGPCRs are not understood. Here we report cryo-electron...
G protein-coupled receptors play essential roles in cellular processes such as neuronal signaling, vision, olfaction, tasting, and metabolism. As GPCRs are the most important drug targets, understanding their interactions with ligands is of utmost importance for discovering related new medicines. In many GPCRs, an allosteric sodium ion next to highly conserved residue D2.50 has been proposed stabilize inactive receptor state by mediating between transmembrane helices. Here, we probed...
G protein-coupled receptors (GPCRs) constitute the largest human protein family with over 800 members, which are implicated in many important medical conditions. Serotonin belong to aminergic GPCR subfamily and play roles physiological psychological activities. Structural biology studies have revealed structures of GPCRs atomic details provide basis for identification investigation potential ligands, interact modulate receptors. Here, an integrative approach combining a focused...
The adenosine A2A receptor (A2AAR) is a prototypical member of the class A subfamily G-protein-coupled receptors (GPCRs) that widely distributed in various tissues and organs human body, participates many important signal-regulation processes. We have previously summarized common activation pathway GPCRs which series conserved residues/motifs undergo conformational change during extracellular agonist binding finally induce coupling intracellular G protein. Through this mechanism we...
Among the various families of G protein-couple receptors (GPCR), adhesion family GPCRs is specialized by its expansive extracellular region, which facilitates recruitment ligands. Previous hypothesis proposed that aGPCRs are activated mechanical force, wherein a Stachel peptide liberated from GPCR autoproteolysis-inducing (GAIN) domain and subsequently binds to transmembrane (7TM) upon activation. In this review, we summarize recent advancements in structural studies aGPCRs, unveiling...
We investigated the role of endocannabinoid system (ECS) in endometrium unexplained infertility (UI) patients, and effect anandamide (AEA) on leukemia inhibitory factor (LIF).Patients were divided into UI control groups. Endometrium samples collected at midluteal phase. Levels cannabinoid type 1 (CB1), fatty acid amide hydrolase (FAAH), LIF examined. productions measured after AEA, CB1 antagonist AM251, CB2 AM630 stimulation.Rates available embryo, successful implantation pregnancy,...
The class B family of G-protein-coupled receptors (GPCRs) has long been a paradigm for peptide hormone recognition and signal transduction. One GPCR, the glucagon-like peptide-1 receptor (GLP-1R), considered as an anti-diabetes drug target there are several peptidic drugs available treatment this overwhelming disease. previously determined structures inactive GLP-1R in complex with two negative allosteric modulators include ten thermal-stabilizing mutations that were selected from total 98...
ABSTRACT The CD93/IGFBP7 axis are key factors expressed in endothelial cells (EC) that mediate EC angiogenesis and migration. Upregulation of them contributes to tumor vascular abnormality blockade this interaction promotes a favorable microenvironment for therapeutic interventions. However, how these two proteins associated each other remains unclear. In study, we solved the human CD93–IGFBP7 complex structure elucidate between EGF 1 domain CD93 IB IGFBP7. Mutagenesis studies confirmed...
Abstract The adenosine subfamily G protein-coupled receptors A 2A R and 2B were identified as promising candidates for cancer immunotherapy within recent years. One of the R/A dual antagonist, AB928, has progressed to phase II clinic trial treatment rectal cancer. However, precise mechanism underlying its dual-antagonistic properties remains elusive. Herein, we report crystal structures in complex with AB928 a selective 2-118. reveal common binding mode on R, wherein ligands establish...
The CD93/IGFBP7 axis are key factors expressed in endothelial cells (EC) that mediate EC angiogenesis and migration. Upregulation of them contributes to tumor vascular abnormality blockade this interaction promotes a favorable microenvironment for therapeutic interventions. However, how these two proteins associated each other remains unclear. In study, we solved the human CD93–IGFBP7 complex structure elucidate between EGF1 domain CD93 IB IGFBP7. Mutagenesis studies confirmed binding...
Abstract Subtype selectivity and functional bias are vital in current drug discovery for G protein-coupled receptors (GPCRs) as selective biased ligands expected to yield leads with optimal on-target benefits minimal side-effects. However, structure-based design medicinal chemistry exploration remain challenging part because of highly conserved binding pockets within subfamilies. Herein, we present an affinity mass spectrometry approach screening herbal extracts identify active a GPCR, the...