A5093632523- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Advanced Proteomics Techniques and Applications
- Polyamine Metabolism and Applications
- Analytical Chemistry and Chromatography
Universität Innsbruck
2024
Amino acids (AAs) and their metabolites are important building blocks, energy sources, signaling molecules associated with various pathological phenotypes. The quantification of AA tryptophan (TRP) in human serum plasma is therefore great diagnostic interest. Therefore, robust, reproducible sample extraction processing workflows as well rapid, sensitive absolute required to identify candidate biomarkers improve screening methods. We developed a validated semi-automated robotic liquid...
Abstract Free amino acids (AAs) and their metabolites are important building blocks, energy sources signaling molecules associated with various pathological phenotypes. The quantification of AA tryptophan (TRP) in human serum plasma is therefore great diagnostic interest. Robust reproducible sample extraction processing workflows as well rapid, sensitive absolute TRP required to identify candidate biomarkers improve current screening methods. We developed a validated semi-automated workflow...
Abstract Tumours face tryptophan (Trp) depletion, but the mechanisms sustaining protein biosynthesis under Trp stress remain unclear. We report that increases levels of translation repressor EIF4EBP1. Yet, at same time, EIF4EBP1 is selectively phosphorylated by metabolic master regulator MTORC1 kinase, preventing from inhibiting translation. activity unexpected because absence amino acids typically linked with inhibition. EIF4EBP1-sensitive in starved cells sustained EGFR and RAS signalling...