A5028330298- Renal Transplantation Outcomes and Treatments
- Blood groups and transfusion
- Transplantation: Methods and Outcomes
- Complement system in diseases
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- Renal Diseases and Glomerulopathies
- Immunodeficiency and Autoimmune Disorders
- Advanced biosensing and bioanalysis techniques
- Piperaceae Chemical and Biological Studies
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Organ Transplantation Techniques and Outcomes
- Blood donation and transfusion practices
- Urinary Tract Infections Management
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Protease and Inhibitor Mechanisms
- Erythrocyte Function and Pathophysiology
- Chronic Lymphocytic Leukemia Research
- Cell Adhesion Molecules Research
Dalhousie University
2016-2025
Nova Scotia Health Authority
2020-2024
Queen Elizabeth II Health Sciences Centre
2009-2023
Canadian Blood Services
2017
Emory University Hospital
2017
Izaak Walton Killam Health Centre
2014-2016
Health Sciences Centre
2013-2014
Arab American Pharmacist Association
2009
Bipar
1999-2001
Tumor cells use activated platelets to promote their proliferation and metastatic potential. Because platelet activation is largely mediated through ADP engagement of purinergic P2Y12 receptors on platelets, we investigated the potential reversible inhibitor ticagrelor, a clinical agent used in prevention cardiovascular cerebrovascular events, inhibit tumor adhesion metastasis. In B16-F10 melanoma intravenous intrasplenic metastasis models, mice treated with dose ticagrelor (10 mg/kg)...
A modified single antigen bead (SAB) assay measuring C1q binding to human leukocyte antibodies has recently been introduced. The aim of this study was investigate the determinants on SAB.Sera from 73 sensitized patients were analyzed by generic IgGpan as well IgG subclass specific SAB assays and correlated with standard an anti-human globulin (AHG) enhanced test.Among 2,665 SABs positive results (mean fluorescence intensity [MFI]>500), strong complement-binding IgG1 IgG3 subclasses accounted...
Technological advances in HLA laboratory testing undoubtedly improved the sensitivity and specificity of antibody assessment but not without introducing a set challenges regarding data interpretation. In particular, introduction solid-phase single-antigen bead (SAB) brought belief that mean fluorescence intensity (MFI) was quantifiable value. As such, MFI levels heavily influenced reporting, monitoring, clinical practice. However, given SAB neither intended for nor approved to be...
Sideroblastic anemias are acquired or inherited that result in a decreased ability to synthesize hemoglobin red blood cells and the presence of iron deposits mitochondria cell precursors. A common subtype congenital sideroblastic anemia is due autosomal recessive mutations SLC25A38 gene. The current treatment for chronic transfusion coupled with chelation. function not known. Here we report protein, its yeast homolog Hem25, mitochondrial glycine transporters required initiation heme...
Dendritic cells (DC) actively rearrange their actin cytoskeleton to participate in formation of the immunological synapse (IS). In this study, we evaluated requirements for DC participation IS. toward naive CD4(+) T only presence specific MHC-peptide complexes. contrast, polarized cytoskeletal proteins absence Ag. rearrangement occurred at same threshold peptide-MHC complexes as that required cell activation. Furthermore, activation was inhibited by blockade rearrangement. When TCR-MHC...
Chemotherapy has been a mainstay in cancer treatment for many years. Despite some success, the cure rate with chemotherapy remains unsatisfactory types of cancers, and severe side effects from these treatments are concern. Recently, understanding dynamic interplay between tumor immune system led to development novel immunotherapies, including vaccines. Cancer vaccines have advantageous features, but their use hampered by poor immunogenicity. Many developments increased potency pre-clinical...
Next-Generation Sequencing (NGS) has transformed clinical histocompatibility laboratories through its capacity to provide accurate, high-throughput, high-resolution typing of Human Leukocyte Antigen (HLA) genes, which is critical for transplant safety and success. As this technology becomes widely used genotyping, now have an increased capability identify novel HLA alleles that previously would not be detected using traditional genotyping methods. Standard guidelines the verification...
ZAP70 [zeta-chain (TCR)-associated protein kinase, 70-kDa], is required for T cell activation. deficiencies in humans and null mutations mice lead to severe combined immune deficiency. Here, we describe a zap70 loss-of-function mutation zebrafish (zap70y442) that was created using transcription activator-like effector nucleases (TALENs). In contrast what has been reported morphant zebrafish, zap70y442 homozygous mutant displayed normal development of blood lymphatic vasculature....
Abstract We have previously reported that oral administration of allogeneic rat spleen cells before kidney allotransplantation significantly prolongs graft survival. This prolongation was alloantigen specific and associated with a decrease in graft-infiltrating (GIC) an increase transcription IL-4 mRNA the GIC. In this study increased splenic mixed lymphocyte responses from animals orally exposed to transplantation suggested allograft not due masking allorecognition, but immunomodulation...
In clinical trials, metronomic cyclophosphamide (CPA) is increasingly being combined with vaccines to reduce tumor-induced immune suppression. Previous strategies modulate the system during vaccination have involved continuous administration of low dose chemotherapy, studies that posed unique considerations for trial design. Here, we evaluated CPA in combination a peptide vaccine targeting HPV16E7 an HPV16-induced tumor model, focusing on cytotoxic T-cell response and timing (mCPA) treatment...
Advances in immunology support the understanding that precise structural epitopes on antibody-accessible region of HLA molecule determine antigenicity and challenge need for identity across full to minimize graft immunogenicity. Retrospective studies confirm quantitative measurement epitope-level mismatching between donor recipient is an informative marker rejection survival suggest prospective allocation organs based this principle may improve survival. Here we describe process rigorous...
Abstract Dendritic cells (DCs) are the most potent APCs for activating naive T cells, a process facilitated by ability of immature DCs to mature and home lymph nodes after encountering an inflammatory stimulus. Proteins involved in cytoskeletal rearrangement play important role regulating adherence motility DCs. Vav1, guanine nucleotide exchange factor Rho family GTPases, mediates hematopoietic following integrin ligation. We show that Vav1 is not required normal maturation vitro; however,...