A5083623904- Renal Transplantation Outcomes and Treatments
- Aquaculture disease management and microbiota
- Physiological and biochemical adaptations
- Fish Ecology and Management Studies
- Organ Transplantation Techniques and Outcomes
- Renal and Vascular Pathologies
- Aquaculture Nutrition and Growth
- Organ Donation and Transplantation
- Transplantation: Methods and Outcomes
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Complement system in diseases
- Renal Diseases and Glomerulopathies
- Air Quality and Health Impacts
- Asthma and respiratory diseases
- Advanced Proteomics Techniques and Applications
- Ocean Acidification Effects and Responses
- Energy and Environment Impacts
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- RFID technology advancements
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Biomedical and Chemical Research
University of British Columbia
2008-2020
EP Analytics (United States)
2018
Prevention of Organ Failure
2009-2014
University of Potsdam
2014
Pacific salmon (Oncorhynchus spp.) serve as good biological indicators of the breadth climate warming effects on fish because their anadromous life cycle exposes them to environmental challenges in both marine and freshwater environments. Our study sought mine extensive functional genomic studies fishes identify robust thermally-responsive biomarkers that could monitor molecular physiological signatures chronic thermal stress using non-lethal sampling gill tissue.Candidate for tissue were...
Background Allergic inflammation is commonly observed in a number of conditions that are associated with atopy including asthma, eczema and rhinitis. However, the genetic, environmental or epigenetic factors involved these likely to be different. Epigenetic modifications, such as DNA methylation, can influenced by environment result changes gene expression. Objectives To characterize methylation pattern airway epithelial cells (AECs) compared peripheral blood mononuclear (PBMCs) discern...
Acute graft rejection is an important clinical problem in renal transplantation and adverse predictor for long term survival. Plasma biomarkers may offer option post-transplant monitoring permit timely effective therapeutic intervention to minimize damage. This case-control discovery study (n = 32) used isobaric tagging relative absolute protein quantification (iTRAQ) technology quantitate plasma concentrations precise cohorts of patients with without biopsy-confirmed acute (BCAR). samples...
Piscine orthoreovirus Strain PRV-1 is the causative agent of heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar). Given its high prevalence net pen salmon, debate has arisen on whether PRV poses a risk to migratory especially British Columbia (BC) where commercially important wild Pacific are decline. Various strains have been associated with diseases including erythrocytic inclusion body syndrome (EIBS), HSMI-like disease, jaundice/anemia Japan, Norway, Chile...
Infectious diseases can impact the physiological performance of individuals, including their mobility, visual acuity, behavior and tolerance ability to effectively respond additional stressors. These effects influence competitiveness, social hierarchy, habitat usage, migratory risk predation, in some circumstances, viability populations. While there are multiple means detecting infectious agents (microscopy, culture, molecular assays), detection wild populations circumstances where mortality...
An organism's ability to respond effectively environmental change is critical its survival. Yet, life stage and overall condition can dictate tolerance thresholds heightened stressors, such that stress may not be equally felt across individuals at all times. Also, the transcriptional responses induced by changes reflect both generalized as well others are highly specific type of being experienced. Thus, if biomarkers a stressor, even under multi-stressor conditions, identified, could then...
Multi-omics research is a key ingredient of data-intensive life sciences research, permitting measurement biological molecules at different functional levels in the same individual. For complete picture systems level, appropriate statistical techniques must however be developed to integrate 'omics' data sets (e.g., genomics and proteomics). We report here multivariate projection-based analyses approaches proteomics sets, using case study applications observations kidney transplant patients...
Early marine survival of juvenile salmon is intimately associated with their physiological condition during smoltification and ocean entry. Smoltification (parr-smolt transformation) a developmental process that allows to acquire seawater tolerance in preparation for living. Traditionally, this has been monitored using gill Na+/K+-ATPase (NKA) activity or plasma hormones, but gene expression offers the possibility another method. Here, we describe discovery candidate genes from tissue...
Abstract Background End-stage renal failure is associated with profound changes in physiology and health, but the molecular causation of these pleomorphic effects termed “uremia” poorly understood. The genomic uremia were explored a whole genome microarray case-control comparison 95 subjects end-stage (n = 75) or healthy controls 20). Methods RNA was separated from blood drawn PAXgene tubes gene expression analyzed using Affymetrix Human Genome U133 Plus 2.0 arrays. Quality control...
Biomarker panels derived separately from genomic and proteomic data with a variety of computational methods have demonstrated promising classification performance in various diseases. An open question is how to create effective proteo-genomic panels. The framework ensemble classifiers has been applied successfully analytical domains combine so that the exceeds individual classifiers. Using blood-based diagnosis acute renal allograft rejection as case study, we address following this paper:...
Aquatic species are increasingly confronted with environmental stressors because of climate change. Although molecular technologies have advanced our understanding how organisms respond to in laboratory settings, the ability detect physiological responses specific under complex field conditions remains underdeveloped. This research applied multi-stressor challenge trials on coho salmon, employing "Salmon Fit-Chips" genomic tool and a random forest-based classification model develop...
Identifying early gene expression responses to hypoxia (i.e., low dissolved oxygen) as a tool assess the degree of exposure this stressor is crucial for salmonids, because they are increasingly exposed hypoxic stress due anthropogenic habitat change, e.g., global warming, excessive nutrient loading, and persistent algal blooms. Our goal was discover validate gill biomarkers specific response in salmonids across multi-stressor conditions. Gill tissue collected from 24 freshwater juvenile...
Abstract The emergence of infectious agents poses a continual economic and environmental challenge to aquaculture production, yet the diversity, abundance, epidemiology aquatic viruses are poorly characterised. In this study, we applied salmon host transcriptional biomarkers identify select fish in viral disease state, but only those that were negative for known based on RT-PCR screening. These selected metatranscriptomic sequencing discover potential pathogens dead dying farmed Atlantic...
Advances in immunology support the understanding that precise structural epitopes on antibody-accessible region of HLA molecule determine antigenicity and challenge need for identity across full to minimize graft immunogenicity. Retrospective studies confirm quantitative measurement epitope-level mismatching between donor recipient is an informative marker rejection survival suggest prospective allocation organs based this principle may improve survival. Here we describe process rigorous...
Acute graft rejection is an important clinical problem in renal transplantation and adverse predictor for long-term survival. Peripheral blood biomarkers that provide evidence of early may offer option posttransplant monitoring, optimize the utility biopsy, permit timely effective therapeutic intervention to minimize damage.In this feasibility study (n=58), we have used gene expression profiling a case-control design compare whole samples between normal subjects (n=20) patients with (n=11)...
Background Recent advances in hardware and software enabled the use of artificial intelligence (AI) algorithms for analysis complex data a wide range daily-life cases. We aim to explore benefits applying AI specific case transplant nephrology: risk prediction severe posttransplant events. For first time, we combine multinational real-world data, which require legal technical protection measures. Objective The German-Canadian NephroCAGE consortium aims develop evaluate processes, tools,...
There is strong evidence that human papillomavirus (HPV) necessary for the development of cervical cancer. A prophylactic HPV vaccine with high reported efficacy was approved in North America 2006.A mathematical model transmission dynamics used to simulate different scenarios natural disease outcomes and intervention strategies. sensitivity analysis performed compensate uncertainties surrounding key epidemiological parameters.The expected impact vaccines have on cancer incidence prevalence...
Abstract Compatibility for human leukocyte antigen (HLA) genes between transplant donors and recipients improves graft survival but prospective matching is rarely performed due to the vast heterogeneity of this gene complex. To reduce complexity, we have combined next-generation sequencing in silico mapping determine population frequencies probabilities 150 antibody-binding eplets across all 11 classical HLA 2000 ethnically heterogeneous renal patients donors. We show that are more common...
A random-sequence peptide microarray can interrogate serum antibodies in a broad, unbiased fashion to generate disease-specific immunosignatures. This approach has been applied cancer detection, diagnosis of infections, and interrogation vaccine response. We hypothesized that there is an immunosignature specific ME/CFS this could aid the diagnosis. studied two subject groups meeting Canadian Consensus Definition ME/CFS. (n = 25) matched control sera were obtained from study. phase 1/2...
Abstract Early marine survival of juvenile salmon is intimately associated with their physiological condition during ocean entry and especially smoltification. Smoltification a developmental parr–smolt transformation allowing to acquire the trait seawater tolerance in preparation for living. Traditionally, this process has been monitored using gill Na + /K ATPase (NKA) activity or plasma hormones, but gene expression can be reliably used. Here, we describe discovery candidate genes from...
Abstract The emergence of infectious agents poses a continual economic and environmental challenge to aquaculture production, yet the diversity, abundance epidemiology aquatic viruses are poorly characterised. In this study, we applied salmon host transcriptional biomarkers identify select fish in viral disease state but only those that also showed be negative for established viruses. This was followed by metatranscriptomic sequencing determine viromes dead dying farmed Atlantic ( Salmo...
In this study, we explored a time course of peripheral whole blood transcriptomes from kidney transplantation patients who either experienced an acute rejection episode or did not in order to better delineate the immunological and biological processes measureable leukocytes that are associated with renal allograft rejection. Using microarrays, generated gene expression data 24 rejectors nonrejectors. We filtered obtain most unambiguous robustly expressing probe sets selected subset clearest...
We have shown that genomic biomarkers in peripheral blood provide evidence of early graft rejection and may offer an important option for posttransplant monitoring, we are working to improve this signature maximize assay performance.This clinical refinement study (n=79) used gene expression profiling a case-control design compare whole samples between normal subjects (n=20) patients with or without (n=39) biopsy-confirmed acute (BCAR).Gene from BCAR before treatment differed significantly...
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