A5079977674- Renal Transplantation Outcomes and Treatments
- T-cell and B-cell Immunology
- Transplantation: Methods and Outcomes
- Immune Cell Function and Interaction
- Organ Transplantation Techniques and Outcomes
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Complement system in diseases
- Cytomegalovirus and herpesvirus research
- Blood groups and transfusion
- Renal Diseases and Glomerulopathies
- Viral Infections and Immunology Research
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Hematopoietic Stem Cell Transplantation
- Polyomavirus and related diseases
- Liver Diseases and Immunity
- Liver Disease and Transplantation
- Platelet Disorders and Treatments
- Systemic Lupus Erythematosus Research
- Hepatitis B Virus Studies
- Blood transfusion and management
- Organ and Tissue Transplantation Research
- Immune Response and Inflammation
- Reproductive System and Pregnancy
University of Pittsburgh Medical Center
2014-2023
University of Pittsburgh
2008-2021
Canadian Blood Services
2013
Mount Sinai Hospital
2013
Bloodworks Northwest
2013
University of Washington
2013
St. Michael's Hospital
2013
University of Toronto
2013
Ospedale Maggiore
2013
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2013
Abstract A critical factor limiting the availability of histocompatible platelet transfusions for alloimmunized, thrombocytopenic patients is large pool HL‐typed donors needed to procure platelets perfectly matched HLA antigens. We have, therefore, investigated effectiveness obtained from having lesser degrees histocompatibility. In 421 administered 59 alloimmunized who were refractory “random donor” platelets, it was found that mismatched 1 or 2 “cross‐reactive” antigens in most instances...
Twenty-seven patients received pulmonary transplants during the period since we began routine use of cytomegalovirus-seronegative blood products for CMV-seronegative recipients. Preoperative serologic status recipient and occurrence cytomegalovirus infection in postoperative were correlated with development obliterative bronchiolitis (OB) as diagnosed by transbronchial biopsy (TBB). Patients included 20 heart-lung 7 double-lung OB occurred 18 27 patients. All 3 CMV seronegative recipients...
Abstract Objective. The inflammatory myopathies are characterized by distinctive autoantibodies that associated with certain clinical features and immunogenetic patterns. Anti–PM‐Scl is one such antibody found in pure myositis, myositis overlap, systemic sclerosis (SSc). Our purpose was to describe the associations of anti–PM‐Scl antibody. Methods. Serum samples from 617 patients various connective tissue diseases were screened for indirect immunofluorescence Ouchterlony double...
There are many highly sensitized patients on the kidney waiting lists of organ exchange organizations because it is difficult to find a crossmatch negative cadaver for these patients. Recently, several protocols have been developed remove donor-specific human leukocyte antigen (HLA) antibodies from serum before transplantation. These approaches, including use intravenous immunoglobulins, plasmapheresis and immunoglobulins (plasmapheresis-cytomegalovirus-immunoglobulin), immunoabsorption,...
In solid organ transplanted patients, annual influenza immunization is strongly recommended because of morbidity and mortality infections. 2009, the rapid spread a novel H1N1 A virus led to accelerated development pandemic vaccines. Switzerland, recipients received one dose seasonal two doses AS03-adjuvanted This situation provided unique opportunity analyze influence adjuvanted vaccines on production de novo anti-HLA antibodies. We prospectively followed independent cohorts including 92 59...
Bronchiolitis obliterans syndrome (BOS) represents a major limitation in lung transplantation. While acuterejection is widely considered the most important risk factor for BOS, impact of HLA-specific antibodies less understood. Of 51 recipients who were prospectively tested during 4.2±1.6-year period, 14 patients developed antibodies. A multi-factorial analysis was performed to correlate prevalence BOS with HLA antibodies, persistent-recurrent acute rejection (ACR-PR),...
Kidney biopsies are being used to evaluate marginal donors, but rigorous statistical validation of this practice with multivariate analysis has not been performed.To analyze histologic parameters in 78 donor for their ability predict graft dysfunction, we a proportional odds model that included both and recipient factors. Glomerulosclerosis was categorized into grades 0, 1, 2, 3, corresponding 1-10%, 11-20%, 21-30% global sclerosis, respectively. The degrees interstitial fibrosis, tubular...
Background. The correlation between antibody production against mismatched donor human leukocyte antigens (HLA) and the number of amino acid sequence mismatches was analyzed in patients who rejected a kidney transplant (n=146). Methods. A similar analysis performed for women paternal HLA their child (n=1,397). (triplet) differences were using HLAMatchmaker algorithm. Results. In both groups, positive found triplet percentage individuals producing antibodies (P <0.0001). If zero present, no...
HLAMatchmaker is a recently developed computer-based algorithm to determine donor-recipient HLA compatibility at the molecular level. Originally designed for highly alloimmunized patients, this based on concept that immunogenic epitopes are represented by amino acid triplets exposed parts of protein sequences HLA-A, -B, and -C chains accessible alloantibodies. Donor determined intralocus interlocus comparisons in polymorphic sequence positions. For most can identify certain mismatched...
Pneumonia due to Pneumocystis carinii (PCP) is regularly encountered in organ allograft recipients who are immunosuppressed prevent rejection. Recipients of lung/heart allografts may be particularly prone pulmonary infection systemic immunosuppression and the fact that defense mechanisms transplanted lung further impaired through tissue incompatibility effects surgery. In this study, we monitored 16 transplant for with using serial bronchoalveolar lavage (BAL) found prevalence 88%. Six...
Abstract A perfect or nearly human leukocyte antigen (HLA) match has been associated with better immediate and long-term survival of diseased donor kidney transplants. However, the effect HLA matching for hepatic allografts remains poorly defined. Using data from National Institutes Diabetes Digestive Kidney Diseases Liver Transplantation Database, we investigated association between mismatches allograft survival, disease recurrence, immunosuppression interactions. A, B, DR loci were used to...
The concept that HLA antibodies are specific for epitopes rather than antigens is important not only the determination of mismatch acceptability sensitized patients but also a better understanding antibody response to an mismatch. Numerous publications describe epitope-specific antibodies, there no standardized information about repertoire clinically relevant epitopes. Under auspices 16th IHIW, we have developed website-based registry antibody-verified Epitope notations based on molecular...
Defining HLA mismatch acceptability of organ transplant donors for sensitized recipients has traditionally been based on serologically defined antigens. Now, however, it is well accepted that antibodies specifically recognize a wide range epitopes present antigens and molecularly high resolution alleles corresponding to the same low antigen can possess different epitope repertoires. Hence, determination compatibility at allele level represents more accurate approach identify suitable...