A5000549821- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Mechanical Circulatory Support Devices
- Cardiac Structural Anomalies and Repair
- Polyomavirus and related diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tracheal and airway disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Organ and Tissue Transplantation Research
- Cardiac Ischemia and Reperfusion
- Heme Oxygenase-1 and Carbon Monoxide
- Cardiac Arrest and Resuscitation
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Cardiac Valve Diseases and Treatments
- Viral Infections and Immunology Research
- Antifungal resistance and susceptibility
- Xenotransplantation and immune response
- Pulmonary Hypertension Research and Treatments
- Virus-based gene therapy research
- Infective Endocarditis Diagnosis and Management
- Hydrogen's biological and therapeutic effects
- Cardiac and Coronary Surgery Techniques
- Immune Response and Inflammation
Cleveland Clinic
2016-2025
Cleveland Clinic Lerner College of Medicine
2014-2024
Creative Commons
2023
Cerner (United States)
2020-2021
San Diego Cardiac Center
2020
Cleveland Foundation
2017-2018
University of Southern California
2015
Case Western Reserve University
2014
University of Chicago
2014
Cardiovascular Institute of the South
2014
We characterize a line of transgenic pigs that express the human complement-regulatory proteins CD59 and decay-accelerating factor. These genes, under control heterologous promoters, are expressed in variety organs, including vasculature heart, kidney, liver. demonstrate moderate levels these gene products sufficient to protect peripheral blood cells from or baboon complement. Using pig heterotopic heart transplants, we show expression is block complement-mediated damage hallmark such...
Background. Alemtuzumab is being increasingly used for the prevention and/or treatment of acute allograft rejection in organ transplant recipients. We assessed risks infection in, to our knowledge, largest cohort and broadest range recipients yet reported have received alemtuzumab. Methods. All patients who alemtuzumab from September 2002 through March 2004, either as induction therapy at time transplantation or rejection, were evaluated development an opportunistic (OI) until death 12...
Many proteins are associated with the outer layer of cell membrane through a posttranslationally added glycosyl phosphatidylinositol (GPI) anchor. The functional significance this type protein linkage is unclear, although it results in increased lateral mobility, sorting to apical surface cell, reinsertion into membranes, and possibly signaling. Here evidence presented that GPI-linked can undergo intermembrane transfer vivo. expressed on transgenic mouse red blood cells were transferred form...
Conventional regimens of immunosuppressive drugs often do not prevent chronic rejection after lung transplantation. Topical delivery cyclosporine in addition to conventional systemic immunosuppression might help acute and events.We conducted a single-center, randomized, double-blind, placebo-controlled trial inhaled initiated within six weeks transplantation given immunosuppression. A total 58 patients were randomly assigned inhale either 300 mg aerosol (28 patients) or placebo (30 three...
<h2>Abstract</h2> <b>Background:</b> Solid organ transplant (SOT) recipients have been reported to be more susceptible influenza virus. However, little is known about the clinical epidemiology and implications of viral infection among SOT recipients. <b>Methods:</b> Cohort study in at University Pittsburgh Medical Center. <b>Results:</b> Between November 1990 April 2000, 30 cases were diagnosed our center, including A (n = 22) B 8). These included lung 19), liver 5) kidney 6) transplants....
Lung transplant recipients have one of the highest rates invasive aspergillosis (IA) in solid organ transplantation. We used a single center, nonrandomized, retrospective, sequential study design to evaluate fungal infection lung who were managed with either universal prophylaxis voriconazole (n = 65) or targeted 30) itraconazole +/- inhaled amphotericin patients at high risk (pre- posttransplant Aspergillus colonization [except niger]). The rate IA 1 year was better receiving as compared...
The clinical utility of Platelia trade mark Aspergillus galactomannan antigen for the early diagnosis invasive aspergillosis was prospectively assessed in 70 consecutive lung transplant recipients. Sera were collected twice weekly and tested galactomannan. Invasive documented 17.1% (12/70) patients. Using generalized estimating equation model, at cutoff value >or= 0.5, sensitivity test 30%, specificity 93% with positive negative likelihood ratios 4.2 0.75, respectively. Increasing to 0.66...
The clinical utility of Platelia Aspergillus enzyme immunoassay (EIA) for galactomannan (GM) antigen detection in bronchoalveolar lavage (BAL) the diagnosis invasive aspergillosis (IA) lung transplant recipients is not known.BAL fluid samples from consecutive who underwent bronchoscopy were prospectively analyzed GM.A total 333 BAL 116 patients tested. Invasive was documented 5.2% (6/116) patients. Samples included 9 BALs two with proven IA, 19 four probable and 305 110 without IA. At index...
3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) are widely used antilipidemic agents that also immunomodulatory. We evaluated possible effects of these after lung transplantation by comparing outcomes 39 allograft recipients, who were prescribed statins for hyperlipidemia, with those 161 contemporaneous control recipients did not receive drugs. Acute rejection (>or= Grade II) was less frequently found in the statin group (15.1 versus 25.6% biopsies, p < 0.01). None 15...
Abstract Objective Lung transplantation is a viable, life‐saving intervention for several primary pulmonary disorders complicated by severe lung dysfunction. This study was undertaken to evaluate whether patients with systemic sclerosis (scleroderma), autoimmune rheumatic disorder, would receive similar benefit from this intervention. Methods Survival following examined at 2 university medical centers among 29 scleroderma as compared 70 idiopathic fibrosis (IPF) and 38 arterial hypertension...
Community-acquired respiratory viral infections (RVIs) are common in lung transplant patients and may be associated with acute rejection bronchiolitis obliterans syndrome (BOS). The use of sensitive molecular methods that can simultaneously detect a large panel viruses help better define their effects.Lung recipients undergoing serial surveillance diagnostic bronchoalveolar lavages (BALs) during period 3 years were enrolled. BAL samples underwent multiplex testing for 19 types/subtypes using...
Background. The role of the innate immune system in development thrombotic microangiopathy (TM) after α1,3-galactosyltransferase gene-knockout (GTKO) pig organ transplantation primates is uncertain. Methods. Twelve organs (nine hearts, three kidneys) from GTKO pigs were transplanted into baboons that received no immunosuppressive therapy, partial regimens, or a full regimen based on costimulation blockade. After graft failure, histologic and immunohistologic examinations carried out....
Prior transcriptional studies of atrial fibrillation (AF) have been limited to specific transcripts, animal models, chronic AF, right atria, or small samples. We sought characterize the left transcriptome in human AF distinguish changes related susceptibility and persistence.Left appendages from 239 patients stratified by coronary artery disease, valve history (no sinus rhythm at surgery, surgery) were selected for genome-wide mRNA microarray profiling. Transcripts examined differential...
Bronchiolitis obliterans syndrome (BOS) represents a major limitation in lung transplantation. While acuterejection is widely considered the most important risk factor for BOS, impact of HLA-specific antibodies less understood. Of 51 recipients who were prospectively tested during 4.2±1.6-year period, 14 patients developed antibodies. A multi-factorial analysis was performed to correlate prevalence BOS with HLA antibodies, persistent-recurrent acute rejection (ACR-PR),...
The critical shortage of human donor organs has generated interest in the potential for porcine to xenotransplantation. initial immunological barrier xenotransplantation is hyperacute rejection, which mediated by xenoreactive antibodies and complement, results rapid irreversible tissue destruction. While endogenous complement regulatory proteins (CRPs) protect cells from injury caused autologous they are relatively species specific most likely ineffectual this setting. This led hypothesis...
Abstract Transplants performed between phylogenetically disparate species are subject to hyperacute rejection initiated by binding of xenoreactive natural Abs endothelium in the donor organ. Binding these activates complement, leading tissue injury and destruction graft. Human recognize Gal alpha 1-3Gal beta 1-4GlcNAc (galactose 1-3galactose 1-4-N-acetylglucosame); however, relative importance this Ag graft has not been proved. The present study was conducted test potential alpha-galactosyl...