A5017728565- Neonatal Respiratory Health Research
- Air Quality and Health Impacts
- Phagocytosis and Immune Regulation
- Lipid Membrane Structure and Behavior
- Trace Elements in Health
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell death mechanisms and regulation
- Heavy Metal Exposure and Toxicity
- Inhalation and Respiratory Drug Delivery
- Climate Change and Health Impacts
- Sphingolipid Metabolism and Signaling
- Coenzyme Q10 studies and effects
- Pulmonary Hypertension Research and Treatments
- Medical Imaging and Pathology Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Heme Oxygenase-1 and Carbon Monoxide
- Free Radicals and Antioxidants
- Noise Effects and Management
- Genomics, phytochemicals, and oxidative stress
- Synthesis and biological activity
- Congenital Diaphragmatic Hernia Studies
- Cancer, Hypoxia, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neuroscience and Neuropharmacology Research
- Growth Hormone and Insulin-like Growth Factors
University of Pittsburgh
2012-2025
University of Illinois Urbana-Champaign
2022
University of Inland Norway
2022
University of Occupational and Environmental Health Japan
2012-2014
University of Pennsylvania
1997
University of Vermont
1989-1991
Penn State Milton S. Hershey Medical Center
1987
Pennsylvania State University
1987
Cornell University
1982
New York State College of Veterinary Medicine
1982
Carbon nanotubes (CNT), with their applications in industry and medicine, may lead to new risks human health. CNT induce a robust pulmonary inflammation oxidative stress rodents. Realistic exposures occur conjunction other pathogenic impacts (microbial infections) trigger enhanced responses. We evaluated interactions between pharyngeal aspiration of single-walled (SWCNT) bacterial infection C57BL/6 mice Listeria monocytogenes (LM). Mice were given SWCNT (0, 10, 40 mug/mouse) 3 days later...
Particulate matter 2.5 μm or less in diameter (PM2.5) is associated with adverse outcomes for patients idiopathic pulmonary fibrosis, but its association other fibrotic interstitial lung diseases (fILDs) and the of PM2.5 composition remain unclear.
Oxidative stress is a potential component of the final common pathway leading to apoptosis following many diverse stimuli. Here, we document that oxidant paraquat caused in mouse 32D cells. We examined early paraquat-induced lipid peroxidation after metabolic incorporation oxidant-sensitive fluorescent fatty acid cis-parinaric (cis-PA) into phospholipids and high-performance liquid chromatography separation specific phospholipid classes. Paraquat induced cis-PA primarily phosphatidylserine...
Introduction Residential proximity to unconventional natural gas development (UNGD) has been shown be associated with asthma exacerbations, but there is limited evidence regarding whether exacerbations are a particular distance or phase of well activity.
Gaseous pollutants such as CO, NO2, O3, and SO2 are linked to adverse clinical outcomes in patients with fibrotic interstitial lung diseases (fILDs), particularly idiopathic pulmonary fibrosis. However, the effect of various exposure estimation methods on these findings remains unclear. This study aims evaluate three spatial approaches─nearest neighbor (NN), inverse distance weighting (IDW), Kriging─for estimating gaseous pollutant exposures assess how affect health outcome estimates fILD...
Loss of differentiated function by type II pneumocytes plated on plastic surfaces was demonstrated decreased lamellar body content, increased cellular protein, and rapid flattening, changes that were retarded modestly plating cells laminin-coated surfaces. Laminin also inhibited [3H]thymidine (THM) incorporation into DNA 40% compared with at 40 h, but did not alter an additional mitogenic effect rat serum over fetal calf serum. In contrast, the laminin-rich basement membrane-like gel formed...
Etoposide (VP-16) is extensively used to treat cancer, yet its efficacy calamitously associated with an increased risk of secondary acute myelogenous leukemia. The mechanisms for the extremely high susceptibility myeloid stem cells leukemogenic effects etoposide have not been elucidated. We propose a mechanism account etoposide-induced leukemia and nutritional strategies prevent this complication therapy. hypothesize that phenoxyl radicals (etoposide-O<sup>⋅</sup>) formed from by...
The formation of reactive oxygen species has been associated with apoptosis. To assess the role lipid peroxidation in apoptosis, we used 2,2'-azobis(2,4-dimethylisovaleronitrile) (AMVN) to generate peroxyl radicals within cellular membranes HL-60 cells. cis-Parinaric acid (cis-PnA) metabolically integrated into phospholipids cells was as a probe extent specific phospholipid classes. Within 2 h, AMVN (500 microM) randomly oxidized more than 85% cis-PnA contained all major classes...
The genetic basis for the underlying individual susceptibility to chlorine-induced acute lung injury is unknown. To uncover and pathophysiological processes that could provide additional homeostatic capacities during injury, 40 inbred murine strains were exposed chlorine, haplotype association mapping was performed. identified single-nucleotide polymorphism (SNP) associations evaluated through transcriptomic metabolomic profiling. Using ≥ 10% allelic frequency phenotype explained as...
Several aspects of tissue response to injury, including cell proliferation, migration, and deposition extracellular matrix, have been attributed platelet-derived growth factor (PDGF)-like cytokines. Because these responses play key roles in lung PDGF-B (c-sis) gene expression was measured by Northern blot analysis total RNA prepared after oxidant injury induced chronic exposure rats 85% oxygen for zero, 1, 3, 7 days. Constitutive but low levels mRNA (4.0 kb) were observed the lungs control...
Phosphatidylserine (PS) externalization, a functional end point of apoptosis that triggers phagocytic recognition dying cells, may be modulated by oxidative stress in biological membranes. We previously observed selective oxidation PS during apoptosis, but the intracellular location and molecular mechanisms responsible for remain to described. Peroxidation individual classes cellular phospholipids was monitored whole cells various subcellular fractions obtained from HL-60 undergoing response...
Binding of beta adrenergic receptors (BAR) in membranes freshly isolated type II pulmonary epithelial cells to the radioligand [125I]iodocyanopindolol was saturable, steresospecific and high affinity. Optimal conditions for assay BAR on pneumocyte are presented. Type primarily beta-2 subtype, as indicated by inhibition subtype-specific antagonists, ICI 118,551 betaxolol. Maximum binding Kd were measured (Kd, 4-20 pM; maximum binding, 30-50 fmol/mg protein) used identify factors which alter...
Hypoxia-inducible factor (HIF-1α) and cyclooxygenase-2 (COX-2) have been implicated in the regulation of inflammatory-like processes that lead to angiogenesis fibrotic disorders. Here we demonstrate human lung fibroblasts (HLFs) treated with mixed exposures chemical microbial stimuli, HIF-1α stabilization plays a pivotal role induction COX-2 mRNA protein, driving release vascular endothelial growth (VEGF) proangiogenic profibrotic chemokines. Upon costimulation Ni mycoplasma-derived...
Abstract Scope: This investigation sought to better understand the metabolic role of lung and generate insights into pathogenesis acrolein‐induced acute injury. A respiratory irritant, acrolein is generated by overheating cooking oils or domestic using biomass fuels, in environmental tobacco smoke, a health hazard restaurant workplace. Methods results: Using SM/J (sensitive) 129X1/SvJ (resistant) inbred mouse strains, metabolome was integrated with transcriptome profile before after...