A5047491704- Renal Transplantation Outcomes and Treatments
- Cytomegalovirus and herpesvirus research
- Organ Donation and Transplantation
- Organ Transplantation Techniques and Outcomes
- Renal Diseases and Glomerulopathies
- Complement system in diseases
- RNA Research and Splicing
- Mosquito-borne diseases and control
- Molecular Biology Techniques and Applications
- Intraperitoneal and Appendiceal Malignancies
- Appendicitis Diagnosis and Management
- Transplantation: Methods and Outcomes
- Cancer Genomics and Diagnostics
- SARS-CoV-2 and COVID-19 Research
- Hematopoietic Stem Cell Transplantation
- Blood groups and transfusion
- COVID-19 Clinical Research Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Renal and Vascular Pathologies
- Minimally Invasive Surgical Techniques
- Prenatal Screening and Diagnostics
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Metabolism and Genetic Disorders
- T-cell and B-cell Immunology
University of British Columbia
2014-2025
Vancouver Coastal Health
2023-2024
Vancouver General Hospital
2020
Roslin Institute
2011-2015
University of Edinburgh
2015
St. Paul's Hospital
2014
Royal Veterinary College
2014
University of Cape Town
2013
Leeds General Infirmary
2006
Brunel University of London
2006
Thirty-five children, three months to 14 years of age, with disfiguring port-wine stains were treated a flashlamp-pulsed tunable dye laser. All had complete clearing the after an average 6.5 laser treatments each lesional area; skin over bony prominences required approximately half as many sessions on cheek. Children less than seven old fewer (mean ±SD, 5.8±1.1) older children (7.1±1.1; P<0.05). Treated was identical in texture and color adjacent normal 33 (94.3 percent) whereas 2 (5.7...
Background Despite advances in clinical management, cytomegalovirus (CMV) infection remains a serious complication and an important cause of morbidity mortality following kidney transplantation. Here, we explore the importance viral load kinetics as predictors risk potential guides to therapy reduce transplant failure large longitudinal Genome Canada Transplant Consortium (GCTC) cohort. Methods We examined relationship between CMV rates characteristics, kinetics, graft patient outcomes 2510...
How often does out-of-pocket payment (OPP) for assisted reproduction techniques (ART) with conventional ovarian stimulation result in catastrophic expenditure households? Catastrophic cost was a frequent event affecting 51% of the poorest study participants and one five couples total. There is increasing concern about spending on health by households low resource settings, but to date no has evaluated OPP ART. We conducted prospective observational comprising 135 undergoing ART between March...
Background The use of cardiac biomarkers to assist in the diagnosis occult and symptomatic hypertrophic cardiomyopathy ( HCM ) cats has been established. There is limited data describing their prognostic utility with . Hypothesis Circulating concentrations N‐terminal B‐type natriuretic peptide (NTproBNP) troponin I cTnI predict death Animals Forty‐one diagnosed at a veterinary teaching hospital, between February 2010 May 2011. Methods Prospective investigational study. Plasma samples were...
Semliki Forest virus (SFV) provides a well-characterized model system to study the pathogenesis of encephalitis. Several studies have used derived from molecular clone SFV4. SFV4 does not same phenotype as closely related L10 or prototype which its was derived. In mice, generates high-titer plasma viremia, is efficiently neuroinvasive, and produces fatal panencephalitis, whereas low-dose low-titer rarely enters brain, generally avirulent. To determine genetic differences responsible,...
Next-Generation Sequencing (NGS) has transformed clinical histocompatibility laboratories through its capacity to provide accurate, high-throughput, high-resolution typing of Human Leukocyte Antigen (HLA) genes, which is critical for transplant safety and success. As this technology becomes widely used genotyping, now have an increased capability identify novel HLA alleles that previously would not be detected using traditional genotyping methods. Standard guidelines the verification...
A bstract : Recent evidence suggests that cell‐free plasma DNA has potential use as a prognostic marker in many clinical settings. The aim of the present study was to evaluate role prediction outcome intensive treatment unit (ITU) patients. Cell‐free measured by real‐time polymerase chain reaction assay for β‐globin gene and SOFA score, APACHE II CRP concentrations, (duration stay, ventilation time, mortality) were noted 94 patients on admission ITU. median concentration ITU 5493 GE/mL this...
<h3>Background</h3> COPD is a complex chronic disease with poorly understood pathogenesis. Integrative genomic approaches have the potential to elucidate biological networks underlying and lung function. We recently combined genome-wide genotyping gene expression in 1111 human specimens map quantitative trait loci (eQTL). <h3>Objective</h3> To determine causal associations between function-associated single nucleotide polymorphisms (SNPs) tissue changes our eQTL dataset. <h3>Methods</h3>...
The A7(74) strain of Semliki Forest virus (SFV; genus Alphavirus) is avirulent in adult mice, while the L10 virulent mice all ages. It has been previously demonstrated that this phenotypic difference associated with nonstructural protein 3 (nsP3). Consensus clones (designated SFV6) and A774wt) were used to construct a panel recombinant viruses. insertion nsP3 from A774wt into SFV6 backbone had minor effect on virulence resulting virus. Conversely, or replacement two copies resulted...
Abstract Background There is a need to improve personalized immunosuppression in organ transplantation reduce premature graft loss. More efficient biomarkers are needed better detect rejection, asymptomatic injury, and under-immunosuppression. Assessment of minimal necessary exposure guide tapering prevent immune activation also important. Donor-derived cell-free DNA (dd-cfDNA) has become available for comprehensive monitoring allograft integrity. A value proposition concept was applied...
Advances in immunology support the understanding that precise structural epitopes on antibody-accessible region of HLA molecule determine antigenicity and challenge need for identity across full to minimize graft immunogenicity. Retrospective studies confirm quantitative measurement epitope-level mismatching between donor recipient is an informative marker rejection survival suggest prospective allocation organs based this principle may improve survival. Here we describe process rigorous...
The optimal immunosuppression management in patients with a failed kidney transplant remains uncertain. This study analyzed the association of class II HLA eplet mismatches and maintenance allosensitization after graft failure well characterized cohort 21 who first transplant. A clinically meaningful increase cPRA this was defined as that resulted 50% reduction compatible donor pool measured from time until repeat transplantation, death, or end study. median at 12.13% (interquartile ranges =...
K. R. Sherwood, M. W. Head, Walker, C. Smith, J. Ironside and Fazakerley (2011) Neuropathology Applied Neurobiology37, 633–642 RNA integrity in post mortem human variant Creutzfeldt–Jakob disease (vCJD) control brain tissue Aims: To determine premortem factors affecting quality yield of isolated from the unique archived material UK National Disease Surveillance Unit Brain Tissue Bank to compare this with no neurological disease. Methods: In parallel replicate, was prepared frontal...
Once touted as the future of hip arthroplasty, metal-on-metal (MoM) bearing surfaces have fallen sharply from favor with emergence a strong body evidence demonstrating unacceptably high premature implant failure rates. The previously unpredictable development adverse local tissue reactions (ALTRs) has been substantive contributor to this. Although underlying pathophysiology these so-called "pseudotumors" is now well understood, fundamental predisposing patient risk factors remained...
Abstract Compatibility for human leukocyte antigen (HLA) genes between transplant donors and recipients improves graft survival but prospective matching is rarely performed due to the vast heterogeneity of this gene complex. To reduce complexity, we have combined next-generation sequencing in silico mapping determine population frequencies probabilities 150 antibody-binding eplets across all 11 classical HLA 2000 ethnically heterogeneous renal patients donors. We show that are more common...
Allogenic hematopoietic stem cell transplantation (allo-HSCT) is a life-saving treatment for various hematological disorders. The success of allo-HSCT depends on the engraftment donor cells and elimination recipient monitored through chimerism testing. We aimed to validate next-generation sequencing (NGS)-based assay monitoring emphasize importance including most prevalent subsets in proficiency testing (PT) programs. evaluated analytical performance NGS-based (AlloSeq-HCT CareDx) with panel...
Background Recent advances in hardware and software enabled the use of artificial intelligence (AI) algorithms for analysis complex data a wide range daily-life cases. We aim to explore benefits applying AI specific case transplant nephrology: risk prediction severe posttransplant events. For first time, we combine multinational real-world data, which require legal technical protection measures. Objective The German-Canadian NephroCAGE consortium aims develop evaluate processes, tools,...
To date there is limited data on the immune profile and outcomes of solid organ transplant recipients who encounter COVID-19 infection early post-transplant. Here we present a unique case where kidney recipient’s surgery coincided with positive SARS-CoV-2 test patient subsequently developed symptomatic perioperatively. We performed comprehensive immunological monitoring cellular, proteomic, serological changes during first 4 critical months post-infection. showed that continuation...