A5059484212- Veterinary Oncology Research
- T-cell and B-cell Immunology
- Infectious Diseases and Mycology
- Cell death mechanisms and regulation
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
- Ion Channels and Receptors
- Antimicrobial Peptides and Activities
- Blood donation and transfusion practices
- Adenosine and Purinergic Signaling
- NF-κB Signaling Pathways
- Iron Metabolism and Disorders
- Calcium signaling and nucleotide metabolism
- Cytokine Signaling Pathways and Interactions
- Cutaneous Melanoma Detection and Management
- Retinoids in leukemia and cellular processes
- Cardiac, Anesthesia and Surgical Outcomes
- Melanoma and MAPK Pathways
- Immunotherapy and Immune Responses
- Chronic Myeloid Leukemia Treatments
- Blood transfusion and management
- Hematopoietic Stem Cell Transplantation
- Cardiac Arrest and Resuscitation
- Beetle Biology and Toxicology Studies
- Nitric Oxide and Endothelin Effects
Dalhousie University
2009-2024
Saarland University
2016-2024
Health Sciences Centre
2013-2024
Universitätsklinikum des Saarlandes
2024
Laboklin (Germany)
2021-2023
Queen Elizabeth II Health Sciences Centre
2013-2021
University of Colorado Denver
2021
Allgemeiner Deutscher Automobil Club
2020
Nova Scotia Health Authority
2019
University Medical Center Freiburg
2018
Bovine lactoferricin (LfcinB) is a cationic, amphipathic peptide that cytotoxic for human and rodent cancer cells. However, the mechanism by which LfcinB causes death of cells not well understood. Here, we show in vitro treatment with rapidly induced apoptosis several different leukemia carcinoma cell lines as determined DNA fragmentation assays phosphatidylserine headgroup inversion detected Annexin V binding to surface Importantly, did adversely affect viability untransformed lymphocytes,...
The resistance of many human cancers to immune-based therapies, including adoptive immunotherapy and the administration therapeutic cancer vaccines, has been attributed tumor-associated immune suppression, due in part immunosuppressive molecules located within tumor microenvironment. Adenosine is a purine nucleoside found interstitial fluid solid tumors at concentrations that are able inhibit cell-mediated responses cells. It well established extracellular adenosine inhibits T lymphocyte...
Abstract Adenosine is a purine nucleoside with immunosuppressive activity that acts through cell surface receptors (A1, A2a, A2b, A3) on responsive cells such as T lymphocytes. IL-2 major growth and survival factor responsible for inducing Jak1, Jak3, STAT5 phosphorylation, well causing to translocate the nucleus bind regulatory elements in genome. In this study, we show adenosine suppressed IL-2-dependent proliferation of CTLL-2 by inhibiting STAT5a/b tyrosine phosphorylation associated...
Monocytes adjust deadly reactive oxygen species production by altering the subunit composition of ORAI calcium channel.
BACKGROUND The optimal method of providing transfusion medicine (TM) education has not been determined. Transfusion Camp was established in 2012 at the University Toronto as a centrally delivered TM program for postgraduate trainees. impact on knowledge, attitudes, and self‐reported behavior evaluated. METHODS Didactic lectures (delivered locally, by webinar, or recorded) locally facilitated team‐based learning seminars were over 5 days during academic year to 8 sites: 7 Canada 1 United...
A 48-year-old woman attended a physician because of solitary cutaneous nodule on the left lower leg. Microscopic examination excisional specimen revealed dermal tumor composed nests epithelioid cells exhibiting clear cytoplasm. They had centrally located vesicular nuclei with distinct nucleoli. rich network capillaries was present throughout. The showed an infiltrative border. There no epidermal involvement. Periodic acid-Shif (PAS) and PAS-Diastase stains demonstrated glycogen deposition...
Abstract Multiple myeloma is a common hematological malignancy that urgently requires new approaches to treatment, since the disease not curable using current chemotherapeutic regimens. The aim of this study was determine whether human and mouse multiple cells are killed by pleurocidin-like cationic antimicrobial peptides NRC-03 NRC-07, previously shown be active against breast cancer cells. We demonstrate here NRC-07 bound rapidly causing extensive membrane damage, as well DNA cleavage....
Breed predispositions to canine digital neoplasms are well known. However, there is currently no statistical analysis identifying the least affected breeds. To this end, 2912 amputated digits submitted from 2014–2019 Laboklin GmbH & Co. KG for routine diagnostics were statistically analyzed. The study population consisted of 155 different breeds (most common: 634 Mongrels, 411 Schnauzers, 197 Labrador Retrievers, 93 Golden Retrievers). Non-neoplastic processes present in 1246 (43%),...
Canine digital melanoma, in contrast to canine oral is still largely unexplored at the molecular genetic level. The aim of this study was detect mutant genes melanoma. Paraffin-embedded samples from 86 melanomas were examined for BRAF V595E variant by droplet PCR (ddPCR), and exon 11 mutations c-kit. Furthermore, exons 2 3 KRAS NRAS analysed Sanger sequencing. Copy number variations (CNV) KITLG genomic DNA nine dogs. absent c-kit, a single nucleotide polymorphism found 16/70 tumours (23%)....
Abstract Ryanodine receptors (RyR) are involved in regulating intracellular Ca ++ mobilization T lymphocytes. However, the importance of RyR signaling during cell activation has not yet been determined. In this study, we have used RyR‐selective antagonists, ruthenium red and dantrolene, to determine effect blockade on receptor‐mediated events cytokine‐dependent proliferation. Both dantrolene inhibited DNA synthesis division, as well interleukin (IL)‐2 by lymphocytes responding mitogenic...
Cutaneous neurocristic hamartoma (NCH) is a rare, pigmented lesion consisting of cells that aberrantly develop from the neurocrest. In addition to dermal melanocytic component, NCH can also harbor neurosustentacular and neuromesenchymal components. has many features in common with other neoplasm, such as blue nevi. Recognition important not only because potential for misinterpretation melanoma, but melanoma arise within these lesions over an unpredictable time frame remain undetected deep...
Purpose Retinal vein occlusion (RVO) has been investigated in several laser-induced animal models using pigs, rabbits and rats. However, RVO rarely reported mice, despite the impressive number of available mutants, ease handling cost effectiveness. The aim this study was to further assess feasibility a mouse model for gene expression analysis its possible use investigate effects hypoxia. Methods C57Bl/6J mice were injected with eosin Y photo-sensitization. Subsequently, large retinal veins...
Thy‐1 (CD90) expressed by mouse T cells is known to have signal transducing properties, but the ability of enhance cytotoxic lymphocyte (CTL) development not well understood. Here we show that stimulation with monoclonal antibodies (mAb) CD3, CD28 and (clone G7), which were coimmobilized on polystyrene microbeads, resulted in a greater proliferative response than only anti‐CD3 anti‐CD28 mAb, indicating cross‐linking enhanced cell receptor/CD28‐driven activation. Consistent this finding,...
To understand inventory performance, transfusion services commonly use key performance indicators (KPIs) as summary descriptors of efficiency that are graphed, trended, and used to benchmark institutions.Here, we summarize current limitations in KPI-based evaluation blood bank propose process mining an ideal methodology for application management research improve flows performance.The transit a product from receipt final disposition is complex relates many internal external influences, KPIs...
Objective: Critical care nurses work in a challenging intensive (ICU) environment that results work-related psychological distress. Our objective was to pilot an in-person or virtual mindfulness-based cognitive therapy (MBCT) program enhanced resilience and similarly designed attention control group. Methods: We randomized ICU with symptoms of burnout syndrome decreased MBCT formatted book club control. primary outcome change as measured by the Connor-Davidson Resilience Scale (CD-RISC)....
Grading, immunohistochemistry and c-kit mutation status are criteria for assessing the prognosis therapeutic options of canine cutaneous mast cell tumours (MCTs). As a subset, digital MCTs have rarely been explored in this context. Therefore, retrospective study, 68 paraffin-embedded were analysed, histological grading was assessed according to Patnaik Kiupel. The immunohistochemical markers KIT Ki67 used, as well polymerase chain reaction (PCR) mutational screening exons 8, 9, 11 14....