A5003160859- Eicosanoids and Hypertension Pharmacology
- Acute Kidney Injury Research
- Peroxisome Proliferator-Activated Receptors
- Renal and related cancers
- Cancer, Hypoxia, and Metabolism
- Metabolism and Genetic Disorders
- Advanced Glycation End Products research
- Chronic Kidney Disease and Diabetes
- Dialysis and Renal Disease Management
- Pluripotent Stem Cells Research
- Diet and metabolism studies
- Biochemical Acid Research Studies
- Renin-Angiotensin System Studies
- Estrogen and related hormone effects
- Hormonal Regulation and Hypertension
- Renal cell carcinoma treatment
- Pesticide Exposure and Toxicity
- Glutathione Transferases and Polymorphisms
- Ion Transport and Channel Regulation
- Organ Transplantation Techniques and Outcomes
- Pharmacogenetics and Drug Metabolism
- Carcinogens and Genotoxicity Assessment
- 3D Printing in Biomedical Research
- Neonatal Respiratory Health Research
- Congenital heart defects research
University of Pittsburgh
2018-2024
Children's Hospital of Pittsburgh
2022-2023
University of Pittsburgh Medical Center
2023
Beijing Tian Tan Hospital
2018
Capital Medical University
2018
Shantou University
2018
Shantou University Medical College
2018
Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, mimicry, ultimately transplantation hinge on achieving robust vascularization of vitro engineered organs. Here, focusing human kidney organoids, we overcame this hurdle by combining induced pluripotent stem (iPSC) line containing an inducible ETS translocation variant 2 (ETV2) (a transcription factor playing endothelial development) that directs differentiation vitro, with non-transgenic iPSC...
Dicarboxylic fatty acids are generated in the liver and kidney a minor pathway called acid ω-oxidation. The effects of consuming dicarboxylic as an alternative source dietary fat have not been explored. Here, we fed dodecanedioic acid, 12-carbon (DC12), to mice at 20% daily caloric intake for nine weeks. DC12 increased metabolic rate, reduced body fat, improved glucose tolerance. We observed DC12-specific breakdown products liver, kidney, muscle, heart, brain, indicating that oral escaped...
Significance Statement In this study, we demonstrate that a common, low-cost compound known as octanedioic acid (DC 8 ) can protect mice from kidney damage typically caused by ischemia-reperfusion injury or the chemotherapy drug cisplatin. This seems to enhance peroxisomal activity, which is responsible for breaking down fats, without adversely affecting mitochondrial function. DC not only affordable and easy administer but also effective. These encouraging findings suggest could potentially...
Abstract Acute kidney injury (AKI) manifests as a major health concern, particularly for the elderly. Understanding AKI‐related proteome changes is critical prevention and development of novel therapeutics to recover function mitigate susceptibility recurrent AKI or chronic disease. In this study, mouse kidneys were subjected ischemia‐reperfusion injury, contralateral remained uninjured enable comparison assess injury‐induced in proteome. A ZenoTOF 7600 mass spectrometer was optimized...
Succinylation is an understudied posttranslational modification that has been shown to increase peroxisomal activity. Furthermore, increased activity reduce oxidative stress and protect proximal tubules after acute kidney injury. Analysis of mass spectrometry succinylomic proteomic data reveals a novel role for Parkinson’s related Park7 in mediating Nrf2 antioxidant response This protection pathway provides new insights injury prevention development therapeutics.
Phosphine has been claimed to cause chromosomal damage at exposures close the current time weighted average exposure standard of 0.3 ppm (0.4 mg/m3). The study involved 31 phosphine fumigators and 21 controls during high fumigation season. All were volunteers evaluated for genotoxicity variables, including micronuclei in peripheral blood lymphocytes urine mutagenicity. In parallel, all 17 full haematology, multiple biochemical analysis, whole organochlorines, serum cholinesterase activity....
Acute kidney injury (AKI) is associate with high mortality. Despite evidence of AKI-induced distant organ injury, a relationship between AKI and liver has not been clearly established. The goal this study to investigate whether renal ischemia-reperfusion (IR) can affect pathophysiology. We showed that IR in mice induced fatty compromised function through the downregulation constitutive androstane receptor (CAR; −90.4%) inhibition hepatic very-low-density lipoprotein triglyceride (VLDL-TG)...
Significance Statement Studies have suggested that estrogens may protect mice from AKI. Estrogen sulfotransferase ( SULT1E1 , or EST) plays an important role in estrogen homeostasis by sulfonating and deactivating estrogens, but studies of ’s AKI are lacking. Using the ischemia-reperfusion model AKI, authors demonstrated genetic ablation pharmacologic inhibition Sult1e1 can mitigate both male female a sex hormone-independent manner. A gene profiling analysis indicated renoprotective effect...
Abstract Hemorrhagic shock (HS) is a potential life-threatening condition that may lead to injury multiple organs, including the lung. The estrogen sulfotransferase (EST, or SULT1E1) conjugating enzyme sulfonates and deactivates estrogens. In this report, we showed expression of Est was markedly induced in liver but not lung female mice subject HS resuscitation. Genetic ablation pharmacological inhibition effectively protected from HS-induced acute (ALI), interstitial edema, neutrophil...
Acute kidney injury (AKI) manifests as a major health concern, particularly for the elderly. Understanding AKI-related proteome changes is critical prevention and development of novel therapeutics to recover function mitigate susceptibility recurrent AKI or chronic disease. In this study, mouse kidneys were subjected ischemia-reperfusion injury, contralateral remained uninjured enable comparison assess injury-induced in proteome. A fast-acquisition rate ZenoTOF 7600 mass spectrometer was...
Abstract Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, mimicry, ultimately transplantation clinical setting thereby hinges on achieving robust vascularization of vitro engineered organs. Here, focusing human kidney organoids, we overcome this hurdle by combining an inducible ETS translocation variant 2 ( ETV2 ) induced pluripotent stem (iPSC) line, which directs endothelial fate, with non-transgenic iPSC line suspension organoid culture....
Introduction Lysine succinylation is a post-translational modification associated with the control of several diseases, including acute kidney injury (AKI). It suggested that hypersuccinylation favors peroxisomal fatty acid oxidation (FAO) instead mitochondrial. In addition, medium-chain acids (MCFAs) dodecanedioic (DC 12 ) and octanedioic 8 ), upon FAO, generate succinyl-CoA, resulting in hypersuccinylation. DC convenient, inexpensive, easily administered, efficient. We believe this study...
Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, mimicry, transplantation hinge on achieving robust vascularization of vitro engineered organs. Here, focusing human kidney organoids, we overcome this hurdle by combining an inducible ETS translocation variant 2 (ETV2) induced pluripotent stem (iPSC) line, which directs endothelial fate, with non-transgenic iPSC line suspension culture. The resulting organoids show extensive cells, more...
Acute kidney injury (AKI) is characterized by a severe and sudden impairment in renal filtration function. A common feature AKI organ-crosstalk, which perturbs the homeostasis of distant organs. Previous studies have suggested that diseases may impair expression activity drug metabolizing enzymes, but mechanism has not been fully elucidated. To understand effect on liver, we established mouse model bilateral ischemia-reperfusion (IRI). Our results showed hepatic estrogen sulfotransferase...