A5080447389- Pediatric health and respiratory diseases
- Identification and Quantification in Food
- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Meat and Animal Product Quality
- Mast cells and histamine
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Mercury impact and mitigation studies
- Respiratory and Cough-Related Research
- Probiotics and Fermented Foods
- Advanced Chemical Sensor Technologies
- Biochemical and Structural Characterization
- Inflammasome and immune disorders
- Inflammation biomarkers and pathways
- Biosensors and Analytical Detection
- Neonatal Respiratory Health Research
- interferon and immune responses
- Smoking Behavior and Cessation
- Influenza Virus Research Studies
- Protein Hydrolysis and Bioactive Peptides
- Respiratory Support and Mechanisms
- Genomics and Phylogenetic Studies
- Immune Response and Inflammation
Hunter Medical Research Institute
2013-2023
University of Newcastle Australia
2013-2023
Universiti Putra Malaysia
2015-2021
Serdang Hospital
2019
Chronic obstructive pulmonary disease (COPD) and influenza virus infections are major global health issues. Patients with COPD more susceptible to infection, which exacerbates their condition increases morbidity mortality. The mechanisms of increased susceptibility remain poorly understood, current preventions treatments have substantial limitations.To characterize the infection in potential for therapeutic targeting.We used a combination primary bronchial epithelial cells (pBECs) from...
Chronic obstructive pulmonary disease (COPD) is the third leading cause of morbidity and death imposes major socioeconomic burdens globally. It a progressive disabling condition that severely impairs breathing lung function. There lack effective treatments for COPD, which direct consequence poor understanding underlying mechanisms involved in driving pathogenesis disease. Toll-like receptor (TLR)2 TLR4 are implicated chronic respiratory diseases, including asthma fibrosis. However, their...
Abstract Toll-like receptor 7 (TLR7) is known for eliciting immunity against single-stranded RNA viruses, and increased in both human cigarette smoke (CS)-induced, experimental chronic obstructive pulmonary disease (COPD). Here we show that the severity of CS-induced emphysema COPD reduced TLR7-deficient mice, while inhalation imiquimod, a TLR7-agonist, induces without CS exposure. This imiquimod-induced mice deficient mast cell protease-6, or when wild-type are treated with stabilizer,...
Food manufacturers across the world commonly add animal substances in their food products. Some products may contain porcine including pork, gelatine, blood and pepsin. These significantly affect texture, colour or taste of end Aside from enhancing sensory qualities, additional ingredients also contribute to preservation, bulk nutrition. However, inclusion might not be suitable among certain communities. One primary concern is fraud labelling which includes hiding addition food. Therefore,...
Klebsiella pneumoniae are opportunistic bacteria found in the gut. In recent years they have been associated with nosocomial infections. The increased incidence of multiple drug-resistant K . makes it necessary to find new alternatives treat disease. this study, phage UPM2146 was isolated from a polluted lake which can lyse its host ATCC BAA-2146. Observation TEM shows that belongs Caudoviriales (Order) based on morphological appearance. Whole genome analysis showed comprises 160,795 bp...
Inhibitory activity of antimicrobial protein against food-borne pathogen such as Listeria monocytogenes offers wide benefits for potential application in food and medical industries. However, various physicochemical properties complexity foods demands discovery new especially from newly isolated bacterial strain. We have previously obtained an unidentified pure culture originated milk curd with excellent antilisterial activity. Here, we aim to identify the bacterium exerting determine...
The usage of porcine pepsin or other derivatives in food products is a common practice European, American and certain Asian countries although it creates issues religious personnel health concerns. In this study, was detected using indirect ELISA that involved the anti-pep80510 polyclonal antibody raised against specific peptide pepsin, pep80510. sensitivity assay for standard 0.008 µg/g. immunoassay did not cross-react to animal rennet milk proteins except microbial coagulant from Mucor...
Detection of porcine plasma using indirect ELISA was developed mAb B4E1 for the prevention their usage in human food that creates religious and health conflicts. The immunoassay has a CV < 20% did not cross-react to other meat non-meat proteins. sensitivity assay is 0.25% (w/w) spiked raw cooked fish surimi. produce false positive result any commercial surimi tested were contain plasma. Determination 60-kDa antigenic protein blood Western blot confirmed its presence fraction blood. Further...
Abstract Cigarette smoke-induced chronic obstructive pulmonary disease (COPD) is a life-threatening inflammatory disorder of the lung. The development effective therapies for COPD has been hampered by lack an animal model that mimics human in short time-frame.We have created mouse cigarette develops hallmark features condition amount time. Tightly controlled amounts smoke were delivered to airways mice, and pathological assessed. roles macrophages mast cell (MC) tryptase pathogenesis...
Abstract Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide and causes significant healthcare economic burden. Cigarette smoking a major risk factor. There lack effective treatments for COPD due to poor understanding underlining mechanisms. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) implicated in respiratory diseases such as asthma fibrosis. However, role TRAIL pathogenesis unknown. In this study, mRNA expression and/or protein...
Abstract Toll-like receptor (TLR)7 is known for eliciting immunity against single-stranded RNA viruses. TLR7 was increased in both human and cigarette smoke (CS)-induced experimental chronic obstructive pulmonary disease (COPD). Severity of CS-induced emphysema COPD reduced TLR7-deficient mice whilst inhalation imiquimod (TLR7-agonist) induced naïve mice. Imiquimod-induced treated with mast cell stabilizer cromolyn or deficient protease-6. Therapeutic treatment anti-TLR7 monoclonal antibody...
Abstract The authors have withdrawn this preprint due to erroneous posting.
The rise in the number of drug-resistant bacteria that can resist almost all kinds antibiotics is due to overuse these (e.g., carbapenems). Thus, there a need find an alternative antibiotic treatment such as use phages. In this study, phage UPM1705 was isolated from polluted lake which lyse its host Klebsiella pneumoniae ATCC BAA-1705. Based on morphological appearance transmission electron microscopy, belongs Caudovirales (Myoviridae). reach titer 107 PFU/ml based double-layer method. It...