A5111074018- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Neonatal Respiratory Health Research
- Pediatric health and respiratory diseases
- Air Quality and Health Impacts
- IL-33, ST2, and ILC Pathways
- Influenza Virus Research Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Iron Metabolism and Disorders
- Respiratory viral infections research
- Mast cells and histamine
- Smoking Behavior and Cessation
- Adipose Tissue and Metabolism
- Occupational Health and Performance
- Gestational Diabetes Research and Management
- Climate Change and Health Impacts
- Probiotics and Fermented Foods
- Occupational and environmental lung diseases
- Eicosanoids and Hypertension Pharmacology
- Neuroscience of respiration and sleep
- Inhalation and Respiratory Drug Delivery
- Reproductive tract infections research
- Immune cells in cancer
- Inflammasome and immune disorders
- Air Quality Monitoring and Forecasting
Hunter Medical Research Institute
2016-2025
University of Newcastle Australia
2020-2024
Rationale and Objectives: Iron availability metabolism are important in the pathogenesis of bacterial infections. More recently, links have been reported between iron severity viral In this study, we characterize a crucial relationship IAV infection disease. Methods: Iron-related gene expression was assessed human airway epithelial cells (AEC) infected with IAV. AECs were cultured ferric iron, iron-loaded transferrin, or chelator, deferoxamine (DFO), prior to Mice placed on high diet for 8...
Abstract Toll-like receptor 7 (TLR7) is known for eliciting immunity against single-stranded RNA viruses, and increased in both human cigarette smoke (CS)-induced, experimental chronic obstructive pulmonary disease (COPD). Here we show that the severity of CS-induced emphysema COPD reduced TLR7-deficient mice, while inhalation imiquimod, a TLR7-agonist, induces without CS exposure. This imiquimod-induced mice deficient mast cell protease-6, or when wild-type are treated with stabilizer,...
Graphical abstractAbstractBackgroundMillions of people are exposed to landscape fire smoke (LFS) globally, and inhalation LFS particulate matter (PM) is associated with poor respiratory cardiovascular outcomes. However, how affects function less well understood.ObjectiveWe aimed characterize the pathophysiologic effects representative airway exposure on cardiac asthma outcomes.MethodsLFS was generated using a customized combustion chamber. In 8-week-old female BALB/c mice, low (25 μg/m3,...
Abstract Increased inflammasome responses are strongly implicated in inflammatory diseases; however, their specific roles incompletely understood. Therefore, we sought to examine the of nucleotide‐binding oligomerization domain–like receptor (NLR) family, pyrin domain–containing 3 (NLRP3) and absent melanoma‐2 (AIM2) inflammasomes cigarette smoke–induced inflammation a model experimental chronic obstructive pulmonary disease (COPD). We targeted NLRP3 with inhibitor MCC950 given...
Lung transcriptomics studies in asthma have provided valuable information the whole lung context, however, deciphering individual contributions of airway and parenchyma disease pathogenesis may expedite development novel targeted treatment strategies. In this study, we performed on using a house dust mite (HDM)-induced model experimental that replicates key features human disease. HDM exposure increased expression 3,255 genes, which 212 were uniquely airways, 856 parenchyma, 2187 commonly...
Maternal iron deficiency occurs in 40–50% of all pregnancies and is associated with an increased risk respiratory disease asthma children. We used murine models to examine the effects lower status during pregnancy on lung function, inflammation structure, as well its contribution severity offspring. A low diet impairs increases airway inflammation, alters structure absence presence experimental asthma. further these major features offspring Importantly, a neutrophilic which indicative more...
Abstract Respiratory infections are one of the most common causes illness and morbidity in neonates worldwide. In acute phase known to cause wide-spread peripheral inflammation. However, inflammatory consequences critical neural control centres for respiration have not been explored. Utilising a well characterised model neonatal respiratory infection, we investigated responses within medulla oblongata which contains key regions. Neonatal mice were intranasally inoculated 24 h birth, with...
Influenza A virus (IAV) infection is a major health risk during pregnancy. Whilst vaccination and antiviral agents are widely employed reduce IAV-induced symptoms, they not sufficient to control IAV infections in pregnancy, especially pandemics. Respiratory viruses like IAV, exploit immune alterations that occur including the upregulation of checkpoint proteins (ICPs) PDL1, PD1 CTLA4. We hypothesize blocking expression PDL1 on innate cells will improve maternal immunity following infection....
There is increasing evidence that third hand exposure to e-cigarette vapor (e-vapor) can have detrimental effects on the lungs. However, whether maternal during pregnancy results in harmful changes offspring unknown. Using two different settings (low versus high power), BALB/c mice were subjected e-vapor (e-vapor deposited onto towels, towels changed daily) absence or presence of nicotine, before, during, and after pregnancy. Male adult then infected with mouse-adapted influenza A virus...
Influenza A virus (IAV) infections are increased during pregnancy especially with asthma as a comorbidity, leading to exacerbations, secondary bacterial infections, intensive care unit admissions, and mortality. We aimed define the processes involved in susceptibility severity of IAV pregnancy, asthma. sensitized mice house dust mite (HDM), induced challenged HDM induce allergic airway disease (AAD). At midpregnancy, we infection. assessed viral titers, inflammation, lung antiviral...
<b>Background:</b> Landscape fires affect millions of individuals globally and are becoming increasingly prevalent intense. Increased respiratory cardiac-associated hospital admissions occur during landscape fire events. A greater understanding how smoke (LFS) affects cardiorespiratory disease is required to identify prevention treatment strategies. We developed a unique system assess different exposure levels LFS from specific geographical locations on health. <b>Methods:</b> particulates...
<b>Introduction:</b> The interplay between iron availability, immune responses and microbes is important in the pathogenesis of bacterial infections. However, relationship respiratory viral infections metabolism largely unknown. Here we investigated levels, influenza A virus (IAV) <b>Methods:</b> Human bronchial epithelial cells (BECs) were cultured with ferric ammonium citrate (FAC) prior to infection IAV (H1N1) assess effects increased on human airway cells. BALB/c mice intranasally...
Abstract Toll-like receptor (TLR)7 is known for eliciting immunity against single-stranded RNA viruses. TLR7 was increased in both human and cigarette smoke (CS)-induced experimental chronic obstructive pulmonary disease (COPD). Severity of CS-induced emphysema COPD reduced TLR7-deficient mice whilst inhalation imiquimod (TLR7-agonist) induced naïve mice. Imiquimod-induced treated with mast cell stabilizer cromolyn or deficient protease-6. Therapeutic treatment anti-TLR7 monoclonal antibody...
Abstract The authors have withdrawn this preprint due to erroneous posting.
<b>Introduction:</b> Women are more affected by asthma than men, highlighting a role for female sex hormones in the pathogenesis of asthma. Asthmatic women on oral contraceptive pill (OCP) have improved symptoms and reduced risk. An understanding how OCP influence outcomes may highlight new treatments <b>Methods:</b> The effects fluctuating hormone levels metabolic responses were assessed peripheral blood mononuclear cells (PBMCs). 17β-estradiol, depot-medroxyprogesterone (DMPA) as well...