A5090297779- MicroRNA in disease regulation
- IL-33, ST2, and ILC Pathways
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Eosinophilic Esophagitis
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Psoriasis: Treatment and Pathogenesis
- Immunodeficiency and Autoimmune Disorders
- RNA Interference and Gene Delivery
- Pediatric health and respiratory diseases
- Inflammatory Bowel Disease
- Respiratory and Cough-Related Research
- Advanced Chemical Sensor Technologies
- MXene and MAX Phase Materials
- Bone and Joint Diseases
- Bone Metabolism and Diseases
- Iron Metabolism and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Liver Diseases and Immunity
Michigan Medicine
2024
Hunter Medical Research Institute
2013-2022
University of Newcastle Australia
2013-2021
Abstract Th22 cells are a major source of IL-22 and have been found at sites infection in range inflammatory diseases. However, their molecular characteristics functional roles remain largely unknown because our inability to generate isolate pure populations. We developed novel differentiation assay generated dual IL-22/IL-17A reporter mice compare populations cultured Th17 cells. Il17a fate-mapping transcriptional profiling provide evidence that these never expressed IL-17A, suggesting they...
Asthma is a chronic inflammatory disease of the airways. It characterized by allergic airway inflammation, remodelling, and hyperresponsiveness (AHR). patients, in particular those with or severe asthma, have remodelling that associated accumulation extracellular matrix (ECM) proteins, such as collagens. Fibulin-1 (Fbln1) an important ECM protein stabilizes collagen other proteins. The level Fbln1c, one four Fbln1 variants, which predominates both humans mice, increased serum airways fluids...
Pathogenic bacterial infections of the lung are life threatening and underpin chronic diseases. Current treatments often ineffective potentially due to increasing antibiotic resistance impairment innate immunity by disease processes steroid therapy. Manipulation miRNA directly regulating anti-microbial machinery immune system may boost host defence responses. Here we demonstrate that miR-328 is a key element response pulmonary infection with non-typeable haemophilus influenzae...
Accumulating evidence highlights links between iron regulation and respiratory disease. Here, we assessed the relationship levels regulatory responses in clinical experimental asthma. We show that cell-free are reduced bronchoalveolar lavage (BAL) supernatant of severe or mild–moderate asthma patients correlate with lower forced expiratory volume 1 s (FEV ). Conversely, iron-loaded cell numbers were increased BAL these FEV /forced vital capacity (FVC) ratio. The airway tissue expression...
MicroRNAs are post-transcriptional regulators of gene expression that differentially regulated during development and in inflammatory diseases. A role for miRNAs allergic asthma is emerging further investigation required to determine whether they may serve as potential therapeutic targets. We profiled miRNA murine lungs from an ovalbumin-induced airways disease model, compared animals receiving dexamethasone treatment non-allergic controls. Our analysis identified 29 were significantly...
Background Emerging evidence suggests that non-olfactory tissues and cells can express olfactory receptors (ORs), however, the exact function of ectopic OR expression remains unknown. We have previously shown in mouse models a unique cooperation between interferon-γ (IFN-γ) lipopolysaccharide (LPS) drives activation pulmonary macrophages leads to induction pathogenic responses respiratory tract. Further, through gene array studies, we by these molecules results selective number ORs. In this...
MicroRNAs (miRNAs) are short noncoding RNAs that regulate a broad spectrum of biological processes, including immune responses. Although the contributions miRNAs to function cells beginning emerge, their specific roles remain largely unknown. IL-33 plays an important role in macrophage activation for innate host defense and proinflammatory In this study, we report miR-487b can suppress levels mRNA protein during differentiation bone marrow-derived macrophages (BMDMs). This results inhibition...
Acute exacerbations of asthma represent a major burden disease and are often caused by respiratory infections. Viral infections recognized as significant triggers exacerbations; however, less is understood about the how microbial bioproducts such endotoxin (lipopolysaccharide (LPS)) trigger episodes. Indeed, increased levels LPS have been linked to onset, severity steroid resistance.The goal this study was identify mechanisms underlying bacterial-induced employing surrogate for infection.We...
Respiratory virus infections are often pathogenic, driving severe inflammatory responses. Most research has focused on localized effects of infection and inflammation. However, can induce broad-reaching, systemic changes that only beginning to be characterized. In this study, we assessed the impact acute pneumovirus in C57BL/6 mice bone marrow hematopoiesis. We hypothesized cytokine production lung upregulates myeloid cell response infection. demonstrate a dramatic increase percentages...
Abstract Respiratory syncytial virus (RSV) infection induces asthma exacerbations, which leads to worsening of clinical symptoms and may result in a sustained decline lung function. Exacerbations are the main cause morbidity mortality associated with asthma, significantly contribute asthma-associated healthcare costs. Although glucocorticoids used manage some patients respond them poorly. The underlying mechanisms steroid-resistant exacerbations remain largely unknown. We have previously...
Background The anti-inflammatory effect of an α7nAChR agonist, PNU-282987, has previously been explored in the context inflammatory disease. However, effects PNU-282987 on type 2 innate lymphoid cells (ILC2s)-mediated allergic airway inflammation not yet established. Aims To determine function ILC2s IL-33– or Alternaria Alternata (AA)– induced inflammation. Methods was administered to mice that received recombinant IL-33 AA intranasal challenges. Lung histological analysis and flow cytometry...
Liver inflammation is a common extraintestinal manifestation in inflammatory bowel disease (IBD), yet, the mechanisms driving gut-liver axis remain poorly understood. IBD leads to breakdown integrity of intestinal barrier causing an increase portal and systemic gut-derived antigens, which challenge liver. Here, we examined role platelet activating factor receptor (PAFR) colitis-associated liver damage using dextran sulfate sodium (DSS) anti-CD40-induced colitis models. Both DSS anti-CD40...
Influenza virus (FLU), rhinovirus (RV) and respiratory syncytial (RSV) are the most common acute infections worldwide. Infection can cause severe health outcomes, while therapeutic options limited, primarily relieving symptoms without attenuating development of lesions or impaired lung function. We therefore examined inflammatory response to these with intent identify components that critical drivers immunopathogenesis thus represent potential targets.BALB/c mice were infected FLU, RV RSV,...