A5075604584- Transplantation: Methods and Outcomes
- Respiratory viral infections research
- Organ Transplantation Techniques and Outcomes
- Mechanical Circulatory Support Devices
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Polyomavirus and related diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Tracheal and airway disorders
- Renal Transplantation Outcomes and Treatments
- Cellular transport and secretion
- Pulmonary Hypertension Research and Treatments
- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Medical Imaging and Pathology Studies
- Lipid Membrane Structure and Behavior
- Pneumonia and Respiratory Infections
- Congenital Diaphragmatic Hernia Studies
- Mast cells and histamine
- Antifungal resistance and susceptibility
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Viral Infections and Immunology Research
- Cardiac Structural Anomalies and Repair
- Cytomegalovirus and herpesvirus research
- Click Chemistry and Applications
Baylor College of Medicine
2015-2025
Texas Children's Hospital
2015-2024
Methodist Hospital
2021
Washington University in St. Louis
2021
University of Cincinnati
2020
The University of Texas MD Anderson Cancer Center
2005-2009
Abstract Idiopathic pulmonary fibrosis (IPF) is a lethal lung disease with progressive and death within 2–3 y of diagnosis. IPF incidence prevalence rates are increasing annually few effective treatments available. Inhibition IL-6 results in the attenuation mice. It unclear whether this due to blockade classical signaling, mediated by membrane-bound IL-6Rα, or trans soluble IL-6Rα (sIL-6Rα). Our study assessed role sIL-6Rα IPF. We demonstrated elevations patients mice during onset...
Biochemical and genetic data suggest that synaptotagmin-2 functions as a Ca 2+ sensor for fast neurotransmitter release in caudal brain regions, but animals and/or synapses lacking have not been examined. We now generated mice which the 5′ end of gene was replaced by lacZ. Using β-galactosidase marker, we show that, consistent with previous studies, is widely expressed spinal cord, brainstem, cerebellum, additionally present selected forebrain neurons, including most striatal neurons some...
Although it has been shown that mast cell-deficient mice have diminished innate immune responses against bacteria, the most important immunoprotective factors secreted from activated cells not identified. Mouse cell protease 6 is a tetramer-forming tryptase. This serine abundant in secretory granules and exocytosed upon bacterial challenge. Here we described generation of protease-6-null mouse. Our discovery lacking this neutral cannot efficiently clear Klebsiella pneumoniae their peritoneal...
The clinical course of COVID-19 in pediatric solid organ transplant recipients remains ambiguous. Though preliminary experiences with adult have been published, literature centered on the population is limited. We herein report a multi-center, multi-organ cohort analysis COVID-19-positive ≤ 18 years at time transplant. Data were collected via institutions' respective electronic medical record systems. Local review boards approved this cross-institutional study. Among 5 centers, 26 patients...
The lungs are a common site of serious infection in both healthy and immunocompromised subjects, the most likely route delivery bioterror agent. Since airway epithelium shows great structural plasticity response to inflammatory stimuli, we hypothesized it might also show functional plasticity.To test inducibility lung defenses against bacterial challenge.Mice were treated with an aerosolized lysate ultraviolet-killed nontypeable (unencapsulated) Haemophilus influenzae (NTHi), then challenged...
Development of pulmonary hypertension is a common and deadly complication interstitial lung disease. Little known regarding the cellular molecular mechanisms that lead to in patients with disease, effective treatment options are lacking. The purpose this study was examine adenosine 2B receptor (A2BR) as regulator vascular remodeling secondary fibrosis. To accomplish this, changes were monitored mice exposed bleomycin conjunction genetic removal A2BR or antagonist GS-6201. Results...
There is an unmet need for preclinical models to understand the pathogenesis of human respiratory viruses and predict responsiveness immunotherapies. Airway organoids can serve as
Idiopathic pulmonary fibrosis (IPF) is a deadly lung disease with few therapeutic options. Apoptosis of alveolar epithelial cells, followed by abnormal tissue repair characterized hyperplastic cell formation, pathogenic process that contributes to the progression fibrosis. However, signaling pathways responsible for increased proliferation cells remain poorly understood.To investigate role deoxycytidine kinase (DCK), an important enzyme salvage deoxynucleotides, in fibrosis.DCK expression...
Abstract Anelloviruses are DNA viruses ubiquitously present in human blood. Due to their elevated levels immunosuppressed patients, anellovirus have been proposed as a marker of immune status. We hypothesized that low levels, reflecting relative immunocompetence, would be associated with adverse outcomes pediatric lung transplantation. assayed blood samples from 57 patients multicenter study for alpha‐ and betatorquevirus, two genera. The primary short‐term outcome interest was acute...
Group III pulmonary hypertension (PH) is a highly prevalent and deadly lung disorder with limited treatment options other than transplantation. PH affects patients ongoing chronic injury, such as idiopathic fibrosis (IPF). Between 30 40% of IPF are diagnosed PH. The diagnosis has devastating consequences to these patients, leading increased morbidity mortality, yet the molecular mechanisms involved in development disease remain elusive. Our hypothesis was that hypoxic-adenosinergic system...
Mast cell degranulation is a highly regulated, calcium-dependent process, which important for the acute release of inflammatory mediators during course many pathological conditions. We previously found that Synaptotagmin-2, calcium sensor in neuronal exocytosis, was expressed mast line. postulated this protein may be involved control cell-regulated and we generated Synaptotagmin-2 knock-out mice to test our hypothesis. cells from mutant animal conferred an abnormally decreased passive...
Infants and young children awaiting lung transplantation present challenges that often preclude successful extracorporeal membrane oxygenation support as a bridge to transplantation. Instability of neck cannulas results in the need for intubation, mechanical ventilation, muscle relaxation creating worse transplant candidate. With use Berlin Heart EXCOR (Berlin Heart, Inc) both venoarterial venovenous central cannulation configurations, 5 pediatric patients were successfully bridged...
Abstract Prediction of PTLD after pediatric lung transplant remains difficult. Use EBV VL in WB has been poorly predictive, while measurement BAL fluid suggested to have enhanced utility. The NIH ‐sponsored Clinical Trials Organ Transplantation Children ( CTOTC ‐03) prospectively obtained serial quantitative measurements PCR both and transplantation. Descriptive statistics, contingency analyses, Kaplan‐Meier analyses evaluated possible association between . Of 61 patients, 34 (56%) had an +...
Based on reports in adult lung transplant recipients, we hypothesized that community-acquired respiratory viral infections (CARVs) would be a risk factor for poor outcome after pediatric transplant. We followed 61 recipients 2+ years or until they met composite primary endpoint including bronchiolitis obliterans syndrome/obliterative bronchiolitis, retransplant, death. Blood, bronchoalveolar lavage, and nasopharyngeal specimens were obtained with standard of care visits. Nasopharyngeal from...
We conducted a randomized, placebo-controlled, double-blind study of pediatric lung transplant recipients, hypothesizing that rituximab plus rabbit anti-thymocyte globulin induction would reduce de novo donor-specific human leukocyte antigen antibodies (DSA) development and improve outcomes. serially obtained clinical data, blood, respiratory samples for at least one year posttransplant. analyzed peripheral blood lymphocytes by flow cytometry, serum antibody development, viral infections...
Prospective studies to determine associated risk factors and related outcomes for pulmonary fungal infection (PFI) after pediatric lung transplant (PLT) are lacking.NIH-sponsored Clinical Trials in Organ Transplantation Children enrolled PLT candidates, collecting data prospectively 2 years post-transplant. Demographics, signs/symptoms, radiology, pathology microbiology were collected. Analyses evaluated PFI-related risks outcomes.In 59 PLT, pre-transplant colonization occurred 6 donors 15...