A5072746141- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Solid State Laser Technologies
- Semiconductor Lasers and Optical Devices
- Heme Oxygenase-1 and Carbon Monoxide
- Neuroscience and Neural Engineering
- Receptor Mechanisms and Signaling
- Laser Material Processing Techniques
- Laser Design and Applications
- Photonic Crystal and Fiber Optics
- Advanced Fiber Laser Technologies
- Hemoglobin structure and function
- Redox biology and oxidative stress
- Sulfur Compounds in Biology
- Bone Tissue Engineering Materials
- Semiconductor Quantum Structures and Devices
- Advanced Fiber Optic Sensors
- Photoreceptor and optogenetics research
- Chemical Synthesis and Analysis
- Venomous Animal Envenomation and Studies
- Neonatal Health and Biochemistry
Friedrich Schiller University Jena
2015-2024
Charité - Universitätsmedizin Berlin
2024
Jena University Hospital
2015-2024
FOM University of Applied Sciences for Economics and Management
2015-2024
Universität Hamburg
2024
University Medical Center Hamburg-Eppendorf
2024
RELX Group (United States)
2022
Max Planck Institute for Molecular Biomedicine
2013-2016
EM Photonics (United States)
2015
Max Bergmann Zentrum für Biomaterialien
2006-2014
The SS2 and adjacent regions of the 4 internal repeats sodium channel II were subjected to single mutations involving, mainly, charged amino acid residues. These mutants, expressed in Xenopus oocytes by microinjection cDNA-derived mRNAs, tested for sensitivity tetrodotoxin saxitoxin single-channel conductance. results obtained show that involving 2 clusters predominantly negatively residues, located at equivalent positions segment repeats, strongly reduce toxin sensitivity, whereas residues...
Cumulative oxidative damages to cell constituents are considered contribute aging and age-related diseases. The enzyme peptide methionine sulfoxide reductase A (MSRA) catalyzes the repair of oxidized in proteins by reducing back methionine. However, whether MSRA plays a role process is poorly understood. Here we report that overexpression msrA gene predominantly nervous system markedly extends lifespan fruit fly Drosophila . transgenic animals more resistant paraquat-induced stress, onset...
1. The human eag‐related potassium channel, HERG, gives rise to inwardly rectifying K+ currents when expressed in Xenopus oocytes. 2. apparent inward rectification is caused by rapid inactivation. In extracellular Cs+ solutions, large outward can be recorded having an inactivation time constant at 0 mV of about 50 ms with e‐fold change every 37 mV. 3. HERG channel not amino‐terminal ball structure, as a deletion the cytoplasmic amino terminus (HERG delta 2‐373) did eliminate However,...
Extracellular potassium concentration is actively maintained within narrow limits in all higher organisms. Slight variations extracellular levels can induce major alterations of essential physiological functions excitable tissues. Here we describe that superfusion cultured rat hippocampal neurones with potassium-free medium leads to a decrease specific outward current, probably carried by RCK4-type channels (RCK4 are found brain). This confirmed heterologous expression these Xenopus oocytes....
The antipsychotic drug haloperidol can induce a marked QT prolongation and polymorphic ventricular arrhythmias. In this study, we expressed several cloned cardiac K + channels, including the human ether‐a‐go‐go related gene (HERG) in Xenopus oocytes tested them for their sensitivity. Haloperidol had only little effects on delayed rectifier channels Kv1.1, Kv1.2, Kv1.5 I sK , A‐type channel Kv1.4 inward Kir2.1 (inhibition <6% at 3 μ m haloperidol). contrast, blocked HERG potently with an...
Oxidation of amino acid residues in proteins can be caused by a variety oxidizing agents normally produced cells. The oxidation methionine to sulfoxide is implicated aging as well pathological conditions, and it reversible reaction mediated ubiquitous enzyme, peptide reductase. reversibility suggests that could act cellular regulatory mechanism although no such vivo activity has been demonstrated. We show here residue voltage-dependent potassium channel modulates its inactivation. When this...
1. The potassium channel beta‐subunit from rat brain, Kv beta 1.1, is known to induce inactivation of the delayed rectifier Kv1.1 and Kv1.4 delta 1‐110. 2. 1.1 was co‐expressed in Xenopus oocytes with various other alpha‐subunits. induced members Kv1 subfamily exception 1.6; no 2.1, 3.4 2‐28 Kv4.1 channels could be observed. 3. second member subfamily, 2, had a shorter N‐terminal end, accelerated A‐type 1.4, but did not when rectifiers family. 4. To test whether this subunit co‐assembles...
Ca2+ ions control the cGMP-gated channel of rod photoreceptor cells from external and internal face. We studied ion selectivity blockage by wild-type mutant channels in a heterologous expression system. External blocks inward current at micromolar concentrations highly voltage-dependent manner. The negative membrane voltages shows steep concentration dependence with Hill coefficient approximately 2. face requires 1000-fold higher concentrations. Neutralization glutamate residue (E363)...
Abstract In situ hybridization and Northern blots were used to study the ionotropic subtypes of glutamate receptor in rat hypothalamus. Widespread expression AMPA, kainate, NMDA RNA was found hypothalamus with transcripts same size number as other regions brain. Most glutamate‐receptor subunits studied expressed greater amounts hippocampus than hypothalamus; GluR5, on hand, showed a hippocampus. On basis blot analysis, all brain examined, including hypothalamus, cerebral cortex, cerebellum,...
C-type inactivation of Shaker potassium channels involves entry into a state (or states) in which the inactivated appear nonconducting physiological solutions. However, when channels, from fast N-type has been removed by NH2-terminal deletions, are expressed Xenopus oocytes and evaluated inside-out patches, complete removal K+ ions internal solution exposes conduction Na+ Li+ conformational states. The present paper uses this observation to investigate properties ion through channel states,...
Long-chain polyunsaturated omega-3 fatty acids such as docosahexaenoic acid (DHA), found abundantly in oily fish, may have diverse health-promoting effects, potentially protecting the immune, nervous, and cardiovascular systems. However, mechanisms underlying purported effects of DHA remain largely unclear, part because molecular signaling pathways effectors are only beginning to be revealed. In vascular smooth muscle cells, large-conductance Ca 2+ - voltage-activated K + (BK) channels...
Hypoxia/reoxygenation induces cellular injury by promoting oxidative stress. Reversible oxidation of methionine in proteins involving the enzyme peptide sulfoxide reductase type A (MSRA) is postulated to serve a general antioxidant role. Therefore, we examined whether overexpression MSRA protected cells from hypoxia/reoxygenation injury. Brief hypoxia increased intracellular reactive oxygen species (ROS) level PC12 and promoted apoptotic cell death. Adenovirus-mediated significantly...