A5091222608- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Diabetes Treatment and Management
- Diet, Metabolism, and Disease
- Virus-based gene therapy research
- Pancreatic and Hepatic Oncology Research
- Organ Transplantation Techniques and Outcomes
- Transplantation: Methods and Outcomes
- Xenotransplantation and immune response
- CRISPR and Genetic Engineering
- Renal Transplantation Outcomes and Treatments
- Peroxisome Proliferator-Activated Receptors
- Epigenetics and DNA Methylation
- Cannabis and Cannabinoid Research
- Immunodeficiency and Autoimmune Disorders
- Ion Transport and Channel Regulation
- Endoplasmic Reticulum Stress and Disease
- Digestive system and related health
- Advanced Glycation End Products research
Medizinische Hochschule Hannover
2016-2025
RELX Group (United States)
2018
Saarland University
2016
Institute of Clinical Research
2012
Pancreatic islet beta cell failure causes type 2 diabetes in humans. To identify transcriptomic changes diabetic islets, the Innovative Medicines Initiative for Diabetes: Improving beta-cell function and identification of diagnostic biomarkers treatment monitoring Diabetes (IMIDIA) consortium ( www.imidia.org ) established a comprehensive, unique multicentre biobank human islets pancreas tissues from organ donors metabolically phenotyped pancreatectomised patients (PPP).Affymetrix...
BackgroundInfection may trigger clinically overt mucosal inflammation in patients with predisposition for inflammatory bowel disease. However, the impact of particular enteropathogenic microorganisms is ill-defined. In this study, influence murine norovirus (MNV) infection on clinical, histopathological, and immunological features IL10-deficient (Il10−/−) mouse model disease was examined.
Insulin-producing cells are known for their extremely low antioxidant equipment with hydrogen peroxide (H2O2)-inactivating enzymes. Therefore, catalase was stably overexpressed in mitochondria and comparison the cytoplasmic compartment of insulin-producing RINm5F analyzed its protective effect against toxicity reactive oxygen species (ROS) proinflammatory cytokines. Only mitochondrial overexpression provided protection menadione toxicity, a chemical agent that preferentially generates...
Background/Aims: Elevated levels of non-esterified fatty acids (NEFAs) are under suspicion to mediate β-cell dysfunction and loss in type 2 diabetes, a phenomenon known as lipotoxicity. Whereas saturated show strong cytotoxic effect upon insulin-producing cells, unsaturated not toxic can even prevent toxicity. Experimental evidence suggests that oxidative stress mediates lipotoxicity there is the subcellular site ROS formation peroxisome. However, interaction between NEFAs this process...
The IDDM (LEW.1AR1/Ztm-iddm) rat is a type 1 diabetic animal model characterized by rapid apoptotic pancreatic β-cell destruction. Here we have analyzed the time course of islet infiltration, changes in cytokine expression pattern, and apoptosis transition from pre-diabetic to state. Transition normoglycemia hyperglycemia occurred when loss exceeded 60–70%. At early stages macrophages were predominant immune cell peripherally infiltrated islets. Progression was closely linked severe...
The in vitro toxicity of the diabetogenic agent alloxan as documented by induction beta cell necrosis was studied isolated ob/ob mouse pancreatic islets. effect has been compared with that a number N-alkyl derivatives and structurally related compound, ninhydrin. Alloxan its were selectively toxic to cells, other endocrine cells exocrine parenchymal being well preserved, even at high concentration. In contrast, ninhydrin only comparatively low concentration, destroying all islet types...
Type 1 diabetes (T1D) is an autoimmune disease where pancreatic β-cells are destroyed by islet-infiltrating T cells. Although a role for β-cell defects has been suspected, abnormalities difficult to demonstrate. We show DNA damage response (DDR), presented activation of the 53BP1 protein and accumulation p53, in biopsy autopsy material from patients with recently diagnosed T1D as well rat model human T1D. The DDR more frequent islets infiltrated CD45+ immune cells, suggesting link islet...
Due to shortage of donor tissue a cure for type 1 diabetes by pancreas organ or islet transplantation is an option only very few patients. Gene therapy alternative approach the disease. Insulin generation in non-endocrine cells through genetic engineering promising therapeutic concept achieve insulin independence patients with diabetes. In present study furin-cleavable human was expressed liver autoimmune-diabetic IDDM rats (LEW.1AR1/Ztm-iddm) and streptozotocin-diabetic after portal vein...
Free fatty acids (FFAs) can cause glucose intolerance and diabetes. Lipotoxicity to the pancreatic beta cells is considered be a major underlying for this phenomenon. The aim of study was analyse toxicity profile FFAs in human EndoC-βH1 beta-cell line compare results with isolated rat islets special reference physiologically most prevalent palmitic acid (PA) oleic (OA). Toxicity after 2-day incubation different analysed by caspase-3 assay confirmed propidium iodide annexin V staining tests....
Approximately 10% of patients with type 2 diabetes suffer from latent autoimmune in adults (LADA). This study provides a systematic assessment the pathology endocrine pancreas LADA and for comparison first rat model mimicking characteristics LADA. Islets human pancreases were analyzed by immunohistochemistry immune cell infiltrate composition, situ RT-PCR quantitative real-time PCR laser microdissected islets gene expression proinflammatory cytokines, proliferation marker proliferating...
Scope Lipotoxicity is a significant element in the development of type 2 diabetes mellitus (T2DM). Since pro‐diabetic nutritional patterns are associated with hyperglycemia as well hyperlipidemia, study analyzes effects combining these lipid and carbohydrate components special focus on structural fatty acid properties such increasing chain length (C16–C20) degree saturation regard to role glucolipotoxicity human EndoC‐βH1 β‐cells. Methods results β‐cell death induced by saturated FFAs...
The Cohen diabetes-sensitive rat develops postprandial hyperglycemia when fed a high-sucrose, copper-poor diet, whereas the diabetes-resistant maintains normoglycemia. pathophysiological basis of diabetes was studied in diabetic centering on interplay between exocrine and endocrine compartments pancreas.Studies used male rats 1-month diet. Serum insulin glucose levels were measured during tolerance tests. pancreas evaluated for weight, content, macrophage, fat infiltration....
The prevention of diabetes by the immunomodulatory agent FTY720 (fingolimod) was studied in LEW.1AR1-iddm (IDDM) rat, an animal model human type 1 diabetes. Immune cell subtypes and cytokine profiles pancreatic islets, secondary lymphoid tissue, serum were analyzed for signs immune activation. Animals treated with (1 mg/kg body weight) 40 d starting on 50 life. Changes gene protein expression cytokines, CD8 markers, monocyte chemoattractant protein-1, inducible NO synthase, caspase 3...