A5071942655- Hypothalamic control of reproductive hormones
- Growth Hormone and Insulin-like Growth Factors
- Estrogen and related hormone effects
- Ovarian function and disorders
- Neuroendocrine regulation and behavior
- Reproductive Biology and Fertility
- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Reproductive System and Pregnancy
- Regulation of Appetite and Obesity
- Hormonal Regulation and Hypertension
- Hormonal and reproductive studies
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Birth, Development, and Health
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Plant Molecular Biology Research
- Reproductive Physiology in Livestock
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Sperm and Testicular Function
- Pituitary Gland Disorders and Treatments
- Genetics and Neurodevelopmental Disorders
- Effects and risks of endocrine disrupting chemicals
Hospital de Sant Pau
2024
Institut de Recerca Sant Pau
2024
Oregon Health & Science University
2012-2022
Oregon National Primate Research Center
2013-2022
University of Ulsan
2021
Austral University of Chile
1969-2016
ZRT Laboratory
2000-2011
Texas A&M University
1995-2007
Gobierno de Canarias
2006
Massachusetts General Hospital
1997-2005
To further study the role of GPR54 signaling in onset primate puberty, we used monkey to examine ability kisspeptin-10 elicit release gonadotropin-releasing hormone (GnRH) precociously, and describe expression KiSS-1 hypothalamus during peripubertal period. Agonadal juvenile male monkeys were implanted with a lateral cerebroventricular cannula jugular vein catheter. The responsiveness pituitary endogenous GnRH was heightened chronic pulsatile i.v. infusion synthetic before (112-121)...
Other labs have previously reported the ability of adeno-associated virus serotype 9 (AAV9) to cross blood-brain barrier (BBB). In this report, we carefully characterized variables that might affect AAV9's efficiency for central nervous system (CNS) transduction in adult mice, including dose, vehicle composition, mannitol coadministration, and use single-stranded versus self-complementary AAV. We report AAV9 is able transduce approximately twice as many neurons astrocytes across entire...
Serono Symposia International convened an expert panel to review the impact of environmental influences on regulation pubertal onset and progression while identifying critical data gaps future research priorities. An reviewed literature endocrine-disrupting chemicals, body size, puberty. The concluded that available experimental animal human support a possible role chemicals size in relation alterations boys girls. Critical prioritized for initiatives include (1) etiologic focus...
To date, a therapy for Huntington's disease (HD), genetic, neurodegenerative disorder, remains elusive. HD is characterized by cell loss in the basal ganglia, with particular damage to putamen, an area of brain responsible initiating and refining motor movements. Consequently, patients exhibit hyperkinetic movement disorder. RNA interference (RNAi) offers therapeutic potential this disorder reducing expression HTT, disease-causing gene. We have previously demonstrated that partial...
Gamma-aminobutyric acid (GABA), acting through GABA(A) receptors (GABA(A)R), is hypothesized to suppress reproduction by inhibiting GnRH secretion, but GABA actions directly on neurons are not well established. In green fluorescent protein-identified adult mouse in brain slices, gramicidin-perforated-patch-clamp experiments revealed the reversal potential (E(GABA)) for current GABA(A)Rs was depolarized relative resting potential. Furthermore, rapid application elicited action potentials...
Release of radioimmunoassayable LHRH from hypothalamic fragments adult male rats was measured using an in vitro incubation system. Each flask, containing three tissue 0.5 ml Krebs-Ringer bicarbonate glucose buffer, incubated for 15 min, followed by a 30-min during which either basal release or the effect catecholamines on output evaluated. In some cases, tissues employed consisted fragment medial hypothalamus included median eminence (ME), arcuate and ventromedial nuclei, surrounding...
In both rats and primates, including humans, serum levels of insulin-like growth factor I (IGF-1) increase during the onset puberty, suggesting a role for IGF-I in this process. We examined ability IGF-1 to affect release hypothalamic luteinizing hormone releasing (LHRH) prepubertal female rats. Our results indicate that acts on median eminence, which contains highest density type 1 IGF receptors brain, elicit dose-related LHRH release. regard, minimal effective dose 10 ng/ml (p < 0.05)...
The physiological role of N-methyl-D-aspartate (NMDA) receptors in controlling LH secretion and the initiation puberty was investigated using two specific antagonists, MK-801 DL-2-amino-5-phosphono valeric acid (AP-5). Single daily sc injections (0.1-0.2 mg/kg BW), a noncompetitive NMDA receptor antagonist, given to prepubertal rats significantly delayed but did not prevent timing puberty, as determined by age at vaginal opening first ovulation. Infusion (5 μg/h) via osmotic minipumps for 4...
When the ovaries of 23-day-old juvenile rats are transplanted to an ectopic site, they recover within 1 week ability control gonadotropin secretion via steroid negative feedback. Vascular corrosion casting followed by scanning electron microscopy revealed that ovary becomes profusely revascularized 48 h after transplantation. ingrowth was accompanied a 40- 60-fold increase in expression genes encoding two angiogenic factors, vascular endothelial growth factor (VEGF) and transforming...
Nerve growth factor (NGF) epitomizes a family of proteins known as the neurotrophins (NTs), which are required for survival and differentiation neurons within both central peripheral nervous system. Synthesis NGF in tissues innervated by system is consistent with its function target-derived trophic factor. However, presence low- high-affinity receptors gonads suggests another NTs reproductive endocrine We now report that primordial ovarian follicles, process to occur independently pituitary...
The conversion of androgens to estrogens by aromatase cytochrome P450 (P450AROM) is an important step in the mechanism androgen action brain. In adult rats, P450AROM activity (AA) regulated preoptic area and medial basal hypothalamus, but constitutive amygdala. This study was undertaken determine distribution messenger RNA (mRNA) AA rat brain examine effects steroid treatments on their concentrations various regions. determined a sensitive assay that measures production 3H2O during [1...
A study of the changes in plasma LH and FSH as measured by radioimmunoassay (RIA) has been done maturing male female rats. The concentration patterns a function age (5 to 45 days) were similar two sexes. On contrary, noticeably different. levels rats peaked at around IS days subsequently decreased exponentially. No comparably marked seen male. Hemigonadectomy 10 induces rapid response gonadotropin males. This is especially with for which increased 240% over control value 2 after surgery. In...
We assessed the activity of aromatase enzyme complex in slices brain from rats by measuring release 3H2O [lβ-3H]testosterone. In hypothalami 12-day-old rats, rate was linear with time and amount tissue. The reaction saturated at a substrate concentration 0.1 μM, apparent Km 27 nM. production inhibited 4-hydroxyandrostenedione, an K; 29 Aromatase first detected diencephalon 16-day-old fetuses reached maximum rates hypothalamic tissue between days 18 20 gestation. highest per mg protein (4.8...
Polycystic ovarian syndrome (PCOS) is one of the most common human pathologies affecting women reproductive age. Despite extensive investigation, etiology PCOS remains poorly understood. Experimentally, a PCO-like can be induced in rodents by single dose long-acting estrogen, estradiol valerate (EV). We have used this model to examine possibility that associated with derangement sympathetic control ovary. The release newly incorporated norepinephrine (NE) from nerve terminals response...
Little is known about the presence of trophic factors in hypothalamus and role they may play regulating functional development hypothalamic neurons. We have investigated ability epidermal growth factor (EGF) transforming alpha (TGF-alpha) to affect release luteinizing hormone-releasing hormone (LHRH), neuropeptide that controls reproductive development. also determined whether genes encoding EGF TGF-alpha are expressed prepubertal female hypothalamus. Northern blot analysis poly(A)+ RNA...
ABSTRACT. Medial basal hypothalamic (MBH) and median eminence (ME) fragments from male rats were incubated in Krebs-Ringer bicarbonate-glucose buffer, pH 7.4, at 37 C the prostaglandin Es (PGEs) released into medium measured by RIA. Basal release of PGE ME was 5-6 times greater than that MBH. However, if not separated MBH, MBHME unit similar to MBH alone. In vivo treatment with indomethacin (Id; [l-(p-chlorobenzoyl)-5-methoxy-2-methylindole 3-acetic acid]) inhibit PG synthesis (single sc...