A5078341860- Environmental Toxicology and Ecotoxicology
- Reproductive biology and impacts on aquatic species
- Pharmaceutical and Antibiotic Environmental Impacts
- Fish Ecology and Management Studies
- Effects and risks of endocrine disrupting chemicals
- Zebrafish Biomedical Research Applications
- Toxic Organic Pollutants Impact
- Corporate Governance and Management
- Pesticide and Herbicide Environmental Studies
- Freshwater macroinvertebrate diversity and ecology
- Aquatic Invertebrate Ecology and Behavior
- Aquaculture disease management and microbiota
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Computational Drug Discovery Methods
- Cholinesterase and Neurodegenerative Diseases
- Environmental Chemistry and Analysis
- Pesticide Residue Analysis and Safety
- Heavy Metal Exposure and Toxicity
- Electrochemical Analysis and Applications
- Data Analysis with R
- Insect and Pesticide Research
- Environmental Science and Water Management
- Environmental Science and Technology
- Nanoparticles: synthesis and applications
- Environmental DNA in Biodiversity Studies
Max Planck Institute for Polymer Research
2024-2025
Johannes Gutenberg University Mainz
1993-2025
Fraunhofer Institute for Molecular Biology and Applied Ecology
2015-2024
University Medical Center Hamburg-Eppendorf
2024
Universität Hamburg
2024
Fraunhofer Society
2015
Charité - Universitätsmedizin Berlin
2007
Institute of Molecular Biology
2003
Klinikum Osnabrück
1979
Abstract The aim of the present study was to elucidate how full life‐cycle exposure estrogens impacts zebrafish development and reproduction, compared partial only, whether estrogen‐induced effects in are reversible or irreversible. Zebrafish were exposed a flow‐through system an environmentally relevant concentration (3 ng/L) synthetic estrogen 17α‐ethinylestradiol (EE 2 ) either from fertilization until all‐ovary stage gonad (i.e., 42 d postfertilization [DPF] our experiment) reproductive...
Partial or full life-cycle tests are needed to assess the potential of endocrine-disrupting compounds (EDCs) adversely affect development and reproduction fish. Small fish species such as zebrafish, Danio rerio, under consideration model organisms for appropriate test protocols. The present study examines how reproductive effects resulting from exposure zebrafish synthetic estrogen 17α-ethinylestradiol (EE2) vary with concentration (0.05 10 ng EE2 L−1, nominal), timing/duration (partial...
The present study investigated the biomagnification potential as well substance and tissue-specific distribution of perfluoroalkyl substances (PFASs) in market-size rainbow trout (Oncorhynchus mykiss). Rainbow with an average body weight 314 ± 21 g were exposed to perfluorobutane sulfonate (PFBS), perfluorohexane (PFHxS), perfluorooctane (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic (PFNA) diet for 28 d. accumulation phase was followed by a 28-d depuration phase, which test...
For specific primary modes of action (MoA) in environmental non-target organisms, EU legislation restricts the usage active substances pesticides or biocides. Corresponding regulatory hazard assessments are costly, time consuming and require large numbers non-human animal studies. Currently, predictive toxicology development compounds relies on their chemical structure provides little insights into toxicity mechanisms that precede adverse effects. Using zebrafish embryo model, we...
The potential of photodynamic therapy (PDT) in combination with chemotherapy to improve treatment outcomes for triple-negative breast cancer (TNBC), which no targeted is available, the subject considerable investigation. In PDT, photosensitizers (PSs) are frequently administered directly but do not selectively target cells. To address delivery a PS TNBC and enhance cellular uptake, Ru-NH2-modified avidin bioconjugate (RuAvi) via Tyr-specific modification using Mannich reaction prepared....
The bioaccumulation potential of chemical substances is commonly determined in flow-through fish tests according to the Organization for Economic Cooperation and Development (OECD) technical guideline (TG) 305, aiming at determination bioconcentration factors (BCF). Investigations on aquatic organisms have shown a significant positive correlation between accumulation lipid content organisms. Residue levels can thus differ individuals, species, size groups depending their content. BCF values...
Endocrine disruption (ED) can trigger far-reaching effects on environmental populations, justifying a refusal of market approval for chemicals with ED properties. For the hazard assessment thyroid system, regulatory decisions mostly rely amphibian studies. Here, we used transcriptomics and proteomics identifying molecular signatures interference hormone signaling preceding physiological in zebrafish embryos. this, analyzed 3,3′,5-triiodothyronine (T3) peroxidase inhibitor...
Endocrine disruptors (EDs), capable of modulating the sex hormone system an organism, can exert long-lasting negative effects on reproduction in both humans and environment. For these reasons, properties EDs prevent a substance from being approved for marketing. However, regulatory testing to evaluate endocrine disruption is time-consuming, costly, animal-intensive. Here, we combined sublethal zebrafish embryo assays with transcriptomics proteomics well-characterized disrupting reference...
The Fish Sexual Development Test (FSDT) is a non-reproductive test to assess adverse effects of endocrine disrupting chemicals. With the present study it was intended evaluate whether gene expression endpoints would serve as predictive markers disruption in FSDT. For proof-of-concept, FSDT according OECD TG 234 conducted with non-steroidal aromatase inhibitor fadrozole (test concentrations: 10μg/L, 32μg/L, 100μg/L) using zebrafish (Danio rerio). Gene analyses quantitative RT-PCR were...
Bioconcentration factors (BCF) for regulatory purposes are usually determined by fish flow-through tests according to technical guidance document OECD 305. Fish bioconcentration studies time consuming, expensive, and use many laboratory animals. The aim of this study was investigate whether the freshwater amphipod Hyalella azteca can be used as an alternative test organism studies. Fourteen substances different hydrophobicity (log Kow 2.4–7.6) were tested under conditions determine steady...
As regulatory programs evaluate substances for their endocrine-disrupting properties, careful study design and data interpretation are needed to distinguish between responses that truly endocrine specific those not. This is particularly important in environments where criteria under development identify properties enable hazard-based regulation. Irrespective of these processes, most jurisdictions use the World Health Organization/International Programme on Chemical Safety definition an...
Measurement of specific biomarkers identified by proteomics provides a potential alternative method for risk assessment, which is required to discriminate between hepatotoxicity and endocrine disruption. In this study, adult zebrafish (Danio rerio) were exposed the hepatotoxic substance acetaminophen (APAP) 21 days, in fish short-term reproduction assay (FSTRA). The molecular changes induced APAP exposure studied liver gonads applying previously developed combined FSTRA approach. We observed...
Abstract The aquatic risk assessment for nickel (Ni) in the European Union is based on chronic species sensitivity distributions and use of bioavailability models. To test whether a bioavailability‐based safe threshold Ni (the hazardous concentration 5% [HC5]) protective communities, microcosms were exposed to 5 stable treatments (6–96 μg/L) control 4 mo assess bioaccumulation effects phytoplankton, periphyton, zooplankton, snails. Concentrations macrophytes, snails measured at end exposure...
In the environmental risk assessment of substances, toxicity to aquatic plants is evaluated using, among other methods, 7 dayLemna sp. growth inhibition test following OECD TG 221. So far, not applicable for short-term screening toxicity, nor does it allow evaluation toxic modes action (MoA). The latter also complicated by lack knowledge gene functions in species. Using ecotoxicogenomics, we developed a time-shortened 3 day assay inLemna minor which allows discrimination ecotoxic MoA. By...
In environmental risk assessment of substances, the 14-day growth inhibition test following OECD guideline 239 is employed to assess toxicity in macrophyte Myriophyllum spicatum. Currently, this evaluates physiological parameters and does not allow identification mode action (MoA) by which adverse effects are induced. However, for an improved ecotoxicity knowledge about their ecotoxic MoA non-target organisms required. It has previously been suggested that gene expression changes can...