A5083460926- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Renal and related cancers
- Pluripotent Stem Cells Research
- Testicular diseases and treatments
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Hormonal and reproductive studies
- Wound Healing and Treatments
- Nanoparticle-Based Drug Delivery
- Animal Genetics and Reproduction
- Photoreceptor and optogenetics research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Single-cell and spatial transcriptomics
- Proteoglycans and glycosaminoglycans research
- RNA Interference and Gene Delivery
- Heart Failure Treatment and Management
- Graphene and Nanomaterials Applications
- Nuclear Receptors and Signaling
- Hedgehog Signaling Pathway Studies
- Calpain Protease Function and Regulation
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Glycosylation and Glycoproteins Research
- Neuroinflammation and Neurodegeneration Mechanisms
National Research Council Canada
2024-2025
German Center for Neurodegenerative Diseases
2020-2023
University of Calgary
2016-2022
Abstract Three-dimensional (3D) organoids can serve as an in vitro platform to study cell–cell interactions, tissue development, and toxicology. Development of with architecture similar testis vivo has remained a challenge. Here, we present microwell aggregation approach establish multicellular 3D testicular from pig, mouse, macaque, human. The consist germ cells, Sertoli Leydig peritubular myoid cells forming distinct seminiferous epithelium interstitial compartment separated by basement...
Abstract Background Human pluripotent stem cell‐derived muscle models show great potential for translational research. Here, we describe developmentally inspired methods the derivation of skeletal cells and their utility in tissue engineering with aim to model regeneration dystrophy vitro. Methods Key steps include directed differentiation human embryonic progenitors followed by primary secondary foetal myogenesis into three‐dimensional muscle. To simulate Duchenne muscular (DMD), a...
Chronic infections involving bacterial biofilms pose significant treatment challenges due to the resilience of against existing antimicrobials. Here, we introduce a nanomaterial-based platform for treating Staphylococcus epidermidis biofilms, both in isolation and within biofilm-infected burn skin model. Our approach leverages biocompatible photothermal polydopamine nanoparticles (PDNP), functionalized with branched polyethyleneimine (PEI) loaded antibiotic rifampicin, target bacteria...
An in vitro system to study testicular maturation rats, an important model organism for reproductive toxicity, could serve as a platform high-throughput drug and toxicity screening tissue specific context. In of somatic cells spermatogonia organ culture systems has been reported. However, this challenge organoids derived from dissociated cells. Here, we report generation maintenance rat microwell 28 days. We find that can be maintained only at lower than ambient O 2 tension 15% cultured 34°C...
Inflammatory bowel disease (IBD) is a chronic inflammatory of the gastrointestinal tract with complex and multifactorial etiology, making it challenging to treat. While recent advances in immunomodulatory biologics, such as antitumor necrosis factor-α (TNF-α) antibodies, have shown moderate success, systemic administration antibody therapeutics may lead several adverse effects, including risk autoimmune disorders due cytokine depletion. Transient RNA interference using exogenous short...
Organoids are three dimensional structures composed of multiple cell types that capable recapitulating tissue architecture and functions organs in vivo. Formation organoids has opened up different avenues basic translational research. In recent years, testicular have garnered interest the field male reproductive biology. Testicular allow for study cell-cell interactions, development, germ niche microenvironment facilitate high throughput drug toxicity screening. A method is needed to...
Understanding changes in the expression of genes involved regulating various components neural extracellular matrix (ECM) during aging can provide an insight into aging-associated decline synaptic and cognitive functions. Hence, this study, we compared levels ECM-related hippocampus young, aged very mice. ECM gene was downregulated, despite accumulation proteoglycans aging. The most robustly downregulated carbohydrate sulfotransferase 3 (Chst3), enzyme responsible for chondroitin 6-sulfation...
In mammalian testis, contractile peritubular myoid cells (PMCs) regulate the transport of sperm and luminal fluid, while secreting growth factors extracellular matrix proteins to support spermatogonial stem cell niche. However, little is known about role testicular smooth muscle during postnatal development. Here we report age-dependent expression hypermethylated in cancer 1 (Hic1; also as ZBTB29) cells, including PMCs vascular mouse. Postnatal deletion Hic1 led their increased proliferation...
Abstract Cardiac autonomic neurons control cardiac contractility. Dysregulation of the nervous system can lead to sympathetic overdrive resulting in heart failure and an increased incidence fatal arrhythmias. Here, we introduce innervated engineered human myocardium (iEHM), a novel model neuro-cardiac junctions, constructed by fusion bioengineered neural organoid (BENO) patterned (EHM). Projections into formed presynaptic terminals close proximity cardiomyocytes extensive vascular network...
Organoids are three dimensional structures composed of multiple cell types that capable recapitulating tissue architecture and functions organs in vivo. Formation organoids has opened up different avenues basic translational research. In recent years, testicular have garnered interest the field male reproductive biology. Testicular allow for study cell-cell interactions, development, germ niche microenvironment facilitate high throughput drug toxicity screening. A method is needed to...
Male survivors of childhood cancer are at risk suffering from infertility in adulthood because gonadotoxic chemotherapies. For adult men, sperm collection and preservation routine procedures prior to treatment; however, this is not an option for pre-pubertal children. From young boys, a small biopsy may be taken before chemotherapy, spermatogonia propagated vitro future transplantation restore fertility. A robust system that allows scalable expansion within controlled environment therefore...
Abstract In current clinical practice care of diseased patients is often restricted to separated disciplines. However, such an organ-centered approach not always suitable. For example, cognitive dysfunction a severe burden in heart failure patients. Moreover, these have increased risk for age-associated dementias. The underlying molecular mechanisms are presently unknown and thus corresponding therapeutic strategies improve cognition missing. Using mice as model organisms we show that leads...
ABSTRACT Histone-3-lysine-4-methylation (H3K4me) is mediated by six different lysine methyltransferases (KMTs). Amongst these enzymes SET domain containing 1b (SETD1B) has been linked to intellectual disability but its role in the adult brain not studied yet. Here we show that mice lacking Setd1b from excitatory neurons of forebrain exhibit severe memory impairment. By combining neuron-specific ChIP-seq, RNA-seq and single cell approaches controls expression neuronal-identity genes with a...