A5100410959- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
- Neurogenesis and neuroplasticity mechanisms
- Cardiac Ischemia and Reperfusion
- Nerve injury and regeneration
- Cardiac Structural Anomalies and Repair
- Inflammasome and immune disorders
- NF-κB Signaling Pathways
- Heme Oxygenase-1 and Carbon Monoxide
- Viral Infections and Immunology Research
- Angiogenesis and VEGF in Cancer
- Spinal Cord Injury Research
- Immune cells in cancer
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Aquaculture disease management and microbiota
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Vibrio bacteria research studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Autophagy in Disease and Therapy
Guiyang Medical University
2021-2025
Affiliated Hospital of Guizhou Medical University
2021-2025
The University of Texas Southwestern Medical Center
2014-2024
Mississippi State University
2009-2024
Beijing University of Technology
2023-2024
Hunan University of Science and Technology
2024
The University of Western Australia
2023
The University of Texas MD Anderson Cancer Center
2023
Pellissippi State Community College
2023
Georgetown University Medical Center
2022
The adult mammalian heart has limited potential for regeneration. Thus, after injury, cardiomyocytes are permanently lost, and contractility is diminished. In contrast, the neonatal can regenerate owing to sustained cardiomyocyte proliferation. Identification of critical regulators proliferation quiescence represents an important step toward regenerative therapies. Yes-associated protein (Yap), a transcriptional cofactor in Hippo signaling pathway, promotes embryonic by activating...
Myocardial infarction (MI) leads to cardiomyocyte death, which triggers an immune response that clears debris and restores tissue integrity. In the adult heart, system facilitates scar formation, repairs damaged myocardium but compromises cardiac function. neonatal mice, heart can regenerate fully without scarring following MI; however, this regenerative capacity is lost by P7. The signals govern regeneration are unknown. By comparing MI in mice at P1 P14, we identified differences magnitude...
Background— The clinical use of doxorubicin is limited by cardiotoxicity. Histopathological changes include interstitial myocardial fibrosis and the appearance vacuolated cardiomyocytes. Whereas dysregulation autophagy in myocardium has been implicated a variety cardiovascular diseases, role cardiomyopathy remains poorly defined. Methods Results— Most models cardiotoxicity involve intraperitoneal injection high-dose drug, which elicits lethargy, anorexia, weight loss, peritoneal fibrosis,...
Abstract Following injury, cells in regenerative tissues have the ability to regrow. The mechanisms whereby regenerating adapt injury-induced stress conditions and activate program remain be defined. Here, using mammalian neonatal heart regeneration model, we show that Nrf1, a stress-responsive transcription factor encoded by Nuclear Factor Erythroid 2 Like 1 (Nfe2l1 ) gene, is activated cardiomyocytes. Genetic deletion of Nrf1 prevented cardiomyocytes from activating transcriptional...
Mutations in RNA binding motif protein 20 ( RBM20 ) are a common cause of familial dilated cardiomyopathy (DCM). Many mutations cluster within an arginine/serine-rich (RS-rich) domain, which mediates nuclear localization. These induce mis-localization to form aberrant ribonucleoprotein (RNP) granules the cytoplasm cardiomyocytes and abnormal alternative splicing cardiac genes, contributing DCM. We used adenine base editing (ABE) prime (PE) correct pathogenic p.R634Q p.R636S RS-rich domain...
CRISPR-Cas9 gene editing is emerging as a prospective therapy for genomic mutations. However, current approaches are directed primarily toward relatively small cohorts of patients with specific Here, we describe cardioprotective strategy potentially applicable to broad range heart disease. We used base ablate the oxidative activation sites CaMKIIδ, primary driver cardiac show in cardiomyocytes derived from human induced pluripotent stem cells that CaMKIIδ eliminate oxidation-sensitive...
Although carbon monoxide (CO) has traditionally been viewed as a toxic gas, increasing evidence suggests that it plays an important homeostatic and cytoprotective role. Its therapeutic use, however, is limited by the side effects associated with CO inhalation. Recently, transition metal carbonyls have shown to be safe effective means of transporting releasing groups in vivo. The goal present study was test whether water-soluble CO-releasing molecule, tricarbonylchloro(glycinato) ruthenium...
Enhancing Heart Function The epicardium, a protective layer of tissue surrounding the mammalian heart, plays critical role during embryogenesis because it supplies growth factors and multipotent progenitor cells essential for heart development. In adults, epicardium is dormant but becomes reactivated when injured, response that leads to re-expression developmental genes. Studying mouse models, Huang et al. (p. 1599 , published online 15 November; see Perspective by Rosenzweig ) found C/EBP...
The adult mammalian heart has limited capacity for regeneration following injury, whereas the neonatal can readily regenerate within a short period after birth. Neonatal is orchestrated by multiple cell types intrinsic to heart, as well immune cells that infiltrate injury. To elucidate transcriptional responses of different cellular components mouse we perform single-cell RNA sequencing on hearts at various time points myocardial infarction and couple results with bulk tissue RNA-sequencing...
The adult mammalian heart has limited capacity for regeneration following injury, whereas the neonatal can readily regenerate within a short period after birth. To uncover molecular mechanisms underlying regeneration, we compared transcriptomes and epigenomes of regenerative nonregenerative mouse hearts over 7-d time myocardial infarction injury. By integrating gene expression profiles with histone marks associated active or repressed chromatin, identified transcriptional programs blockade...
Abstract Left ventricular hypertrophy (LVH) is a major risk factor for cardiovascular morbidity and mortality. Pathological LVH engages transcriptional programs including reactivation of canonical fetal genes those inducing fibrosis. Histone lysine demethylases (KDMs) are emerging regulators reprogramming in cancer, though their potential role abnormal heart growth fibrosis remains little understood. Here, we investigate gain loss function an H3K9me2 specific demethylase, Kdm3a , show it...
Inflammasomes are important for host defence against pathogens and homeostasis with commensal microbes. Here, we show non-haemolytic enterotoxin (NHE) from the neglected human foodborne pathogen Bacillus cereus is an activator of NLRP3 inflammasome pyroptosis. NHE a non-redundant toxin to haemolysin BL (HBL) despite having similar mechanism action. Via putative transmembrane region, subunit C initiates binding plasma membrane, leading recruitment B A, thus forming tripartite lytic pore that...
Abstract O -linked β- N -acetyl glucosamine ( -GlcNAc) is at the crossroads of cellular metabolism, including glucose and glutamine; its dysregulation leads to molecular pathological alterations that cause diseases. Here we report -GlcNAc directly regulates de novo nucleotide synthesis nicotinamide adenine dinucleotide (NAD) production upon abnormal metabolic states. Phosphoribosyl pyrophosphate synthetase 1 (PRPS1), key enzyme pathway, -GlcNAcylated by transferase (OGT), which triggers...
The secreted Wnt signaling molecules are essential to the coordination of cell-fate decision making in multicellular organisms. In adult animals, proteins critical for tissue regeneration and frequently contribute cancer. Small that disable acyltransferase Porcupine (Porcn) candidate anticancer agents clinical testing. Here we have systematically assessed effects Porcn inhibitor (WNT-974) on several types identify potentially unwanted chemical could limit therapeutic utility such agents. An...