A5000312967- Inflammasome and immune disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Gout, Hyperuricemia, Uric Acid
- Kawasaki Disease and Coronary Complications
- Hemoglobin structure and function
- Vibrio bacteria research studies
- Advanced Electron Microscopy Techniques and Applications
- Pediatric health and respiratory diseases
- IL-33, ST2, and ILC Pathways
- RNA and protein synthesis mechanisms
- Electron and X-Ray Spectroscopy Techniques
- Rare-earth and actinide compounds
- Monoclonal and Polyclonal Antibodies Research
- Immune responses and vaccinations
- Toxoplasma gondii Research Studies
- Nuclear Physics and Applications
- Viral gastroenteritis research and epidemiology
- Hemoglobinopathies and Related Disorders
- Pneumonia and Respiratory Infections
- Neonatal Health and Biochemistry
- Streptococcal Infections and Treatments
- Superconducting Materials and Applications
- CRISPR and Genetic Engineering
- Iron Metabolism and Disorders
- Erythrocyte Function and Pathophysiology
Australian Regenerative Medicine Institute
2024-2025
Monash University
2024-2025
Australian Research Council
2025
The University of Melbourne
2025
Australian National University
2018-2023
Abstract Moraxella catarrhalis is an important human respiratory pathogen and a major causative agent of otitis media chronic obstructive pulmonary disease. Toll‐like receptors contribute to, but cannot fully account for, the complexity immune response seen in M. infection. Using primary mouse bone marrow‐derived macrophages to examine host infection, our global transcriptomic targeted cytokine analyses revealed activation signalling pathways by both membrane‐bound cytosolic...
Inflammasomes are important for host defence against pathogens and homeostasis with commensal microbes. Here, we show non-haemolytic enterotoxin (NHE) from the neglected human foodborne pathogen Bacillus cereus is an activator of NLRP3 inflammasome pyroptosis. NHE a non-redundant toxin to haemolysin BL (HBL) despite having similar mechanism action. Via putative transmembrane region, subunit C initiates binding plasma membrane, leading recruitment B A, thus forming tripartite lytic pore that...
Inflammasomes are cytosolic signaling complexes capable of sensing microbial ligands to trigger inflammation and cell death responses. Here, we show that guanylate-binding proteins (GBPs) mediate pathogen-selective inflammasome activation. We mouse GBP1 GBP3 specifically required for activation during infection with the bacterium Francisella novicida. selectivity derives from a region within N-terminal domain containing charged hydrophobic amino acids, which binds facilitates direct killing...
High-resolution cryo–electron microscopy (cryo-EM) requires costly 200- to 300-keV cryo–transmission electron microscopes (cryo-TEMs) with field emission gun (FEG) sources, stable columns, constant-powered lenses, autoloader, and direct detectors (DED). Recent advances in 100-keV imaging the emergence of sub–200-keV optimized DED technology promises development more affordable cryo-TEMs. So far, has required FEG sources. We here explored whether a standard 120-keV TEMs thermionic lanthanum...
Haemoglobin (Hb) is a vital oxygen carrier in vertebrates. Low blood Hb levels may indicate anaemia or genetic disorders, while its presence the lower digestive system suggests colon cancer. Detecting and quantifying human essential for medical diagnostics. A nanobody‐based sandwich‐ELISA test was recently developed utilising llama‐derived nanobodies NbE11 NbB9. These specifically bind to without cross‐reacting with from other Here, we determine crystal structure of complex Hb. binds high...
Iron is an essential nutrient for most bacteria and often growth-limiting during infection, due to the host sequestering free iron as part of innate immune response. To obtain required growth, many bacterial pathogens encode transporters capable extracting iron-containing cofactor heme directly from proteins. Pathogenic E. coli Shigella spp. produce outer membrane transporter ChuA, which binds hemoglobin extracts its cofactor, before importing into cell. Heme extraction by ChuA a dynamic...
Abstract Inflammasomes are central to innate immunity mediating host defence against pathogens and maintaining homeostasis with commensal microbes. The sensing of pathogens, danger, or homeostasis-altering signals by inflammasome sensor proteins is the molecular basis driving assembly complex. Here, we identify non-haemolytic enterotoxin (NHE) as an activator NLRP3 inflammasome. We show that NHE, found in neglected human foodborne pathogen Bacillus cereus, a non-redundant toxin haemolysin BL...
Abstract Innate immune recognition of microbial components serves as a cornerstone in mediating an effective response. The inflammasome is intracellular signaling complex comprising sensor, adaptor protein ASC and the cysteine protease caspase-1. Inflammasomes regulate secretion pro-inflammatory cytokines IL-1β IL-18 induction cell death pathway known pyroptosis. Certain bacteria require cytosolic access to activate inflammasome, however, how extracellular are sensed by cytoplasmic remains...