A5060978742- Environmental Monitoring and Data Management
- Alzheimer's disease research and treatments
- Protein Structure and Dynamics
- Scientific Computing and Data Management
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Prion Diseases and Protein Misfolding
- Force Microscopy Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Proteins in Food Systems
- Genetics, Bioinformatics, and Biomedical Research
- Protein purification and stability
- Protein Interaction Studies and Fluorescence Analysis
- Lipid Membrane Structure and Behavior
- Analytical Chemistry and Chromatography
- Cellular Mechanics and Interactions
- Marine and coastal plant biology
- Genomics and Phylogenetic Studies
- Antibiotics Pharmacokinetics and Efficacy
- thermodynamics and calorimetric analyses
- Microfluidic and Capillary Electrophoresis Applications
- Lichen and fungal ecology
- Advanced Biosensing Techniques and Applications
- Cancer Research and Treatments
Life Science Institute
2021-2024
Kanazawa University
2021-2024
Rutgers, The State University of New Jersey
2024
Aalto University
2021-2022
Nagoya Institute of Technology
2020-2021
Osaka University
2008-2020
Protein Research Foundation
2016-2019
Australian National University
2014-2018
University of Tsukuba
2008-2013
Tokyo Institute of Technology
2008
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules their complexes, reporting on size shape in free solution. The purpose this multi-laboratory study was establish the precision accuracy basic data dimensions AUC validate previously proposed calibration techniques. Three kits cell assemblies containing radial temperature tools bovine serum albumin (BSA) reference sample were...
• The role of soil structure on colonization efficiency the pathogen Rhizoctonia solani is analysed by linking experimental techniques that control and quantify with epidemiological concepts deal fungal dynamics colonization. are quantified for colony spreading through replicated samples a nonsterile sandy loam different physical properties. effect bulk-density aggregate-size air-filled pore volume assessed examination thin sections quantification pore-size distributions estimated from...
The nucleation‐growth model has been used extensively for characterizing in vitro amyloid fibril formation kinetics and simulating the relationship between disease. In majority of studies considered as dominant, or sole, aggregation end product, with presence other competing non‐amyloid processes, example amorphous aggregate formation, being largely ignored. Here, we examine possible regulatory effects that off‐pathway processes might exert on rate extent – particular their potential...
Compared to biochemical reactions taking place in relatively well-defined aqueous solutions vitro, the corresponding happening vivo occur extremely complex environments containing only 60–70% water by volume, with remainder consisting of an undefined array bio-molecules. In a biological setting, such and volume-occupied solution are termed ‘crowded’. Through range intermolecular forces pseudo-forces, this background environment may cause behave differently their vitro counterparts. review,...
Amyloid deposits, composed primarily of the 37-residue islet amyloid polypeptide (IAPP), are observed near pancreatic β-cells type II diabetics, with their presence strongly correlating a loss β-cell mass and decreased function. Although membranes have been implicated as likely target amyloidogenic IAPP toxicity, interactions between in fibrillar state not well characterized. In this study, turbidity measurements were conducted to provide detailed description binding reaction fibrils lipid...
Abstract A simple and precise fluorimetric method is described for the simultaneous assay in plasma of a mixture containing chlortetracycline, demethylchlortetracycline tetracycline. Assay within therapeutic ranges 0–5 mg litre−1 achieved by formation strongly fluorescent aluminium/tetracycline complexes, without prior extraction or separation individual antibiotics. This performed determinations at peak excitation fluorescence emission wavelengths each tetracycline chelate. The can be...
Intrinsically disordered domains have been reported to play important roles in signal transduction networks by introducing cooperativity into protein–protein interactions. Unlike intrinsically that become ordered upon binding, the EF-SAM domain stromal interaction molecule (STIM) 1 is distinct it monomeric state and partially unfolded its oligomeric state, with population of two states depending on local Ca2 + concentration. The oligomerization STIM1, which triggers extracellular influx,...
The holdase paradigm of chaperone action involves preferential binding by the to unfolded protein state, thereby preventing it from either, associating with other unstable proteins (to form large dysfunctional aggregates), or being degraded proteolytic machinery cell/organism. In this paper, we examine necessary physical constraints imposed upon response in a cell-like environment and use these limitations comment on likely nature optimal vivo.