A5013455534- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Circadian rhythm and melatonin
- Biochemical Analysis and Sensing Techniques
- Photochromic and Fluorescence Chemistry
- Cardiac electrophysiology and arrhythmias
- Heart Rate Variability and Autonomic Control
- Neurobiology and Insect Physiology Research
Scripps Research Institute
2012-2018
University of Cincinnati Medical Center
2010-2012
Sabin Vaccine Institute
2010-2012
The time course of signaling via heterotrimeric G proteins is controlled through their activation by G-protein coupled receptors and deactivation the action GTPase accelerating (GAPs). Here we identify RGS7 RGS11 as key GAPs in mGluR6 pathway retinal rod ON bipolar cells that set sensitivity light-evoked responses. We showed using electroretinography single cell recordings elimination did not influence dark-adapted responses, but concurrent severely reduced magnitude dramatically slowed...
The cold-sensing channel transient receptor potential melastatin 8 (TRPM8) features Ca(2+)-dependent downregulation, a cellular process underlying somatosensory accommodation in cold environments. functional downregulation of TRPM8 is manifested with two distinctive phases, acute desensitization and tachyphylaxis. Here we show rat dorsal root ganglion neurons that critically depends on phosphatidylinositol 4,5-bisphosphate (PIP(2)) availability rather than PIP(2) hydrolysis triggered by...
Cooling temperatures may modify action potential firing properties to alter sensory modalities. Herein, we investigated how cooling in two groups of rat dorsal root ganglion (DRG) neurons, tetrodotoxin-sensitive (TTXs) Na(+) channel-expressing neurons and tetrodotoxin-resistant (TTXr) neurons. We found that multiple response membrane depolarization was suppressed TTXs but maintained or facilitated TTXr at temperatures. showed strongly inhibited A-type K(+) currents (IA) channels had fewer...
Cone photoreceptors scale dynamically the sensitivity of responses to maintain responsiveness across wide range changes in luminance. Synaptic contribute this adaptation, but how process is coordinated at molecular level poorly understood. Here, we report that a cell adhesion-like molecule, LRIT1, enriched selectively cone photoreceptor synapses where it engages trans-synaptic interaction with mGluR6, principal receptor postsynaptic ON-bipolar cells. The levels LRIT1 are regulated by...
Menthol-sensitive/capsaicin-insensitive neurons (MS/CI) and menthol-sensitive/capsaicin-sensitive (MS/CS) are thought to represent two functionally distinct populations of cold-sensing that use TRPM8 receptors convey innocuous noxious cold information respectively. However, TRPM8-mediated responses have not been well characterized in these neuron populations. Using rat dorsal root ganglion neurons, here we show MS/CI had larger menthol with greater adaptation. In contrast, MS/CS smaller less...
In vertebrate retina, light responses generated by the rod photoreceptors are transmitted to second-order neurons, ON-bipolar cells (ON-BC), and this communication is indispensible for vision in dim light. ON-BCs, synaptic transmission initiated metabotropic glutamate receptor, mGluR6, that signals via G-protein Go control opening of effector ion channel, TRPM1. A key role process belongs GTPase Activating Protein (GAP) complex catalyzes inactivation upon light-induced suppression release...
In the retina, synaptic transmission between photoreceptors and downstream ON-bipolar neurons (ON-BCs) is mediated by a GPCR pathway, which plays an essential role in vision. However, mechanisms that control signal at this synapse its relevance to behavior remain poorly understood. study we used genetic system titrate rate of signaling ON-BC dendrites varying concentration key RGS proteins measuring impact on using electroretinography single cell recordings. We found sensitivity, onset...
The TRPM8 channel is a principal cold transducer that expressed on some primary afferents of the somatic and cranial sensory systems. However, it uncertain whether TRPM8-expressing afferent neurons have ability to convey innocuous noxious stimuli with discrimination between two sub-modalities. Using rat dorsal root ganglion (DRG) patch-clamp recording technique, we characterized membrane action potential properties DRG at 24°C 10°C. could be classified into TTX-sensitive (TTXs/TRPM8)...