A5063028209- Optical Network Technologies
- Advanced Fluorescence Microscopy Techniques
- Advanced Optical Network Technologies
- Advanced Photonic Communication Systems
- Photoreceptor and optogenetics research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Microencapsulation and Drying Processes
- RNA Interference and Gene Delivery
- Hydrogels: synthesis, properties, applications
- Luminescence and Fluorescent Materials
- IoT and Edge/Fog Computing
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Crystallography and molecular interactions
- Advanced NMR Techniques and Applications
- Proteins in Food Systems
- Protein purification and stability
- Conducting polymers and applications
- Photochromic and Fluorescence Chemistry
- Surface Modification and Superhydrophobicity
- Textile materials and evaluations
- High Entropy Alloys Studies
- Indoor and Outdoor Localization Technologies
- Advanced Biosensing Techniques and Applications
Merck & Co., Inc., Rahway, NJ, USA (United States)
2021-2025
Nanjing Tech University
2025
Peking University
2020-2024
Center for Life Sciences
2020-2024
Southwest University of Science and Technology
2024
University of Utah
2024
East China Normal University
2018-2022
Beijing University of Posts and Telecommunications
2010-2020
Anhui Normal University
2020
Nanjing University of Information Science and Technology
2020
Direct delivery of proteins into cells avoids many drawbacks gene delivery, and thus has emerging applications in biotherapy. However, it remains a challenging task owing to limited charges relatively large size proteins. Here, we report an efficient protein system via the co-assembly fluoroamphiphiles nanoparticles. Fluorous substituents on amphiphiles play essential roles formation uniform nanoparticles, avoiding denaturation, endocytosis, maintaining low cytotoxicity. Structure-activity...
Cyclooctatetraene-conjugated cyanine dyes represent an effective strategy to improve biocompatibility under light in live-cell fluorescence imaging and analysis of mitochondria.
The exploration of magnetic resonance imaging (MRI) agents possessing excellent performances and high biosafety is great importance for both fundamental science research biomedical applications. In this study, we present that monodisperse Fe2O3 supraparticles (SPs) can act as T1-weighted MRI agents, which not only possess a distinct off–on switch in the tumor microenvironment but also are readily excreted from living bodies due to its quasi-amorphous structure hierarchical topology design....
Maleimide-cysteine chemistry has been a routine practice for the site-specific labeling of fluorophores to proteins since 1950s. This approach, however, cannot bring out best photon budget fluorophores. Here, we systematically measured Cyanine3/5 dye conjugates via maleimide-thiol and amide linkages by counting total emitted photons at single-molecule level. While brightness signal-to-noise ratios do not change significantly, dyes with thioether exhibit more severe photobleaching than...
As is well known, catalytic performance closely related to the interfacial properties of catalysts. This article introduces metastable In δ + (2 < <3) form an –O V interface, thereby improving performance.
Lipid nanoparticles (LNPs) have emerged as the premier drug delivery system for oligonucleotide vaccines and therapeutics in recent years. Despite their prosperous advancement research clinical applications, there is a significant lack of mechanistic understanding assembly lipid particles at molecular level. In our study, we utilized combination solution solid-state NMR, together with dynamics simulations, to elucidate local structures interactions chemical components across multiple...
Hybrid voltage indicators (HVIs) are chemogenetic sensors that combines the superior photophysical properties of organic dyes and genetic targetability protein to report transient membrane changes. They exhibit boosted sensitivity in excitable cells such as neurons cardiomyocytes. However, signals recorded during long-term imaging severely diminished or distorted due phototoxicity photobleaching issues. To capture stable electrophysiological activities over a long time, we employ cyanine...
In this Letter, a novel five-dimensional (5D) data-iteration-based encryption model is proposed at physical layer for multi-wavelength optical frequency division multiplexing passive network (OFDM-PON) by using hyperchaotic system. The scheme can generate five chaotic sequences time. sensitivity of 10-18 be achieved, along with key space 1095. addition, we use channel to transmit the information, and unit freely choose wavelength. probability shaping technology has greatly improved bit error...
Abstract Rhodamines have been continuously optimized in brightness, biocompatibility, and colors to fulfill the demands of modern bioimaging. However, problem phototoxicity caused by excited fluorophore under long-term illumination has largely neglected, hampering their use time-lapse imaging. Here we introduce cyclooctatetraene (COT) conjugated rhodamines that span visible spectrum exhibit significantly reduced phototoxicity. We identified a general strategy for generation Gentle...
Macrocyclic peptides show promise in targeting high-value therapeutically relevant binding sites due to their high affinity and specificity. However, clinical application is often hindered by low membrane permeability, which limits effectiveness against intracellular targets. Previous studies focused on peptide conformations various solvents, leaving a gap understanding interactions with translocation through lipid bilayers. Addressing this, our study explores the of stapled peptides,...
Rhodamines have been continuously optimized in brightness, biocompatibility, and color to fulfill the demands of modern bioimaging. However, problem phototoxicity caused by excited fluorophore under long-term illumination has largely neglected, hampering their use time-lapse imaging. Here we introduce cyclooctatetraene (COT) conjugated rhodamines that span visible spectrum exhibit significantly reduced phototoxicity. We identified a general strategy for generation Gentle Rhodamines, which...
ABSTRACT Laponite or graphene oxide (GO) is usually used as a multifunctional crosslinker nanofiller to improve the nanocomposite gel strength. To explore strengthening mechanism of GO/Laponite‐based dual hydrogels, we synthesized hydrogel through in situ polymerization acrylamide (AM) dispersion GO and Laponite. The interactions between were confirmed by rheological test. nanosheets exfoliated well dispersed uniformly hydrogels at low concentration GO. Crosslinking network thermal behaviors...
The tough polyacrylamide/Laponite/titania (TiO 2 ) dual nanocomposite (NC) hydrogel was fabricated by in situ radical polymerization of acrylamide (AAm) the dispersion Laponite and TiO . nanoparticles were prepared sol‐gel method used as both inorganic filler multi‐functional crosslinking agent. morphology tuned functions sodium hydroxide (NaOH) concentration ratio Ti (mmol) to clay (g) (mmol Ti/g clay). SEM HR‐TEM morphologies revealed that tended form a uniform regular microstructure like...
Abstract Plasma membrane stains are one of the most important organelle markers for unambiguous assignments individual cells and monitoring morphology dynamics. The state-of-the-art PM bright, specific, fluorogenic, compatible with super-resolution imaging. However, when recording dynamics, particularly under light-intensive microscopes, is prone to photodynamic damages due its phospholipid bilayer nature. Here we developed PK Mem dyes tailored time-lapse fluorescence By integrating...
Abstract Lactate is the highest turnover circulating metabolite in mammals. While traditionally viewed as a waste product, lactate an important energy source for many organs, but first must be oxidized to pyruvate entry into tricarboxylic acid cycle (TCA cycle). This reaction thought occur cytosol, with subsequently transported mitochondria via mitochondrial carrier (MPC). Using 13 C stable isotope tracing, we demonstrated that myocardial tissue of mice even when MPC genetically deleted....
The success of modern biopharmaceutical products depends on enhancing the stability protein therapeutics. Freezing and thawing, which are common thermal stresses encountered throughout lifecycle drug substances, spanning production, formulation design, manufacturing, storage, shipping, can impact this stability. Understanding physicochemical molecular behaviors components in biological at temperatures relevant to manufacturing shipping is essential for assessing risks determining appropriate...
The introduction of solubilizing additives has historically been an attractive approach to address the ever-growing proportion poorly water-soluble drug (PWSD) compounds within modern discovery pipeline. Lipid-formulations, and more specifically micelle formulations, have garnered particular interest because their simplicity, size, scalability, avoidance solid-state limitations. Although formulations widely utilized, molecular mechanism solubilization in surfactant micelles is still...