A5063514825- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Luminescence and Fluorescent Materials
- Photochromic and Fluorescence Chemistry
- Click Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Advanced Electron Microscopy Techniques and Applications
Peking University
2020-2024
Center for Life Sciences
2020-2024
Capturing mitochondria's intricate and dynamic structure poses a daunting challenge for optical nanoscopy. Different labeling strategies have been demonstrated live-cell stimulated emission depletion (STED) microscopy of mitochondria, but orthogonal are yet to be established, image acquisition has suffered either from photodamage the organelles or rapid photobleaching. Therefore, nanoscopy mitochondria largely restricted two-dimensional (2D) single-color recordings cancer cells. Here, by...
Cyclooctatetraene-conjugated cyanine dyes represent an effective strategy to improve biocompatibility under light in live-cell fluorescence imaging and analysis of mitochondria.
Rhodamines have been continuously optimized in brightness, biocompatibility, and color to fulfill the demands of modern bioimaging. However, problem phototoxicity caused by excited fluorophore under long-term illumination has largely neglected, hampering their use time-lapse imaging. Here we introduce cyclooctatetraene (COT) conjugated rhodamines that span visible spectrum exhibit significantly reduced phototoxicity. We identified a general strategy for generation Gentle Rhodamines, which...
Abstract Rhodamines have been continuously optimized in brightness, biocompatibility, and colors to fulfill the demands of modern bioimaging. However, problem phototoxicity caused by excited fluorophore under long-term illumination has largely neglected, hampering their use time-lapse imaging. Here we introduce cyclooctatetraene (COT) conjugated rhodamines that span visible spectrum exhibit significantly reduced phototoxicity. We identified a general strategy for generation Gentle...
Abstract Capturing mitochondria’s intricate and dynamic structure poses a daunting challenge for optical nanoscopy. Different labeling strategies have been demonstrated live-cell stimulated emission depletion (STED) microscopy of mitochondria, but orthogonal are yet to be established, image acquisition has suffered either from photodamage the organelles or rapid photobleaching. Therefore, nanoscopy mitochondria largely restricted 2D single-color recordings cancer cells. Here, by conjugation...