A5033489328- Fuel Cells and Related Materials
- Pancreatic function and diabetes
- ZnO doping and properties
- Membrane-based Ion Separation Techniques
- Cellular transport and secretion
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Advanced battery technologies research
- Copper-based nanomaterials and applications
- Lipid Membrane Structure and Behavior
- Electrocatalysts for Energy Conversion
- Autophagy in Disease and Therapy
- Quantum Dots Synthesis And Properties
- Endoplasmic Reticulum Stress and Disease
- Diabetes and associated disorders
- Near-Field Optical Microscopy
- Ga2O3 and related materials
- Mosquito-borne diseases and control
- Membrane Separation Technologies
- Shape Memory Alloy Transformations
- Ion-surface interactions and analysis
- Advanced X-ray Imaging Techniques
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Virology and Viral Diseases
Institute of Biophysics
2015-2025
Chinese Academy of Sciences
2015-2025
University of Chinese Academy of Sciences
2024
China Three Gorges University
2009-2022
Civil Aviation University of China
2014-2019
Czech Academy of Sciences, Institute of Biophysics
2016
Nanyang Technological University
2013-2016
Academia Sinica
2014
Tianjin University
2010-2011
Zhejiang University
2009
Exosomes are small extracellular 40-100 nm diameter membrane vesicles of late endosomal origin that can mediate intercellular transfer RNAs and proteins to assist premetastatic niche formation. Using primary (SW480) metastatic (SW620) human isogenic colorectal cancer cell lines we compared exosome protein profiles yield valuable insights into factors signaling molecules fundamental tumor progression. purified using OptiPrep™ density gradient fractionation were in diameter, a buoyant ~1.09...
In the conserved autophagy pathway, autophagosomes (APs) engulf cellular components and deliver them to lysosome for degradation. Before fusing with lysosome, APs have close via an unknown mechanism. We previously shown that endocytic Rab5-GTPase regulates AP closure. Therefore, we asked whether ESCRT, which catalyzes scission of vesicles into late endosomes, mediates topologically similar process sealing. Here, show depletion representative subunits from all ESCRT complexes causes defects...
We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine spatial relationship between proteins their native cellular structures. Several fluorescent (FPs) were found be photoswitchable emitted far more photons under our imaging condition, resulting in higher localization precision which is comparable ambient imaging. Vitrified specimens prepared by high pressure freezing cryo-sectioning maintain a near-native state with...
The majority of diabetic patients develop neuropathy and there is an increasing prevalence neurodegeneration in the central nervous system (CNS). However, mechanism behind this poorly understood. Here we first observed that macroautophagy/autophagy was suppressed hippocampus GK rats with hyperglycemia, whereas it unchanged ob/ob mice without hyperglycemia. Autophagy could be directly inhibited by high glucose mouse primary hippocampal neurons. Moreover, autophagy protective...
Emerging microbe infections, such as Zika virus (ZIKV), pose an increasing threat to human health. Investigations on ZIKV replication have revealed the construction of complexes (RCs), but role cytoskeleton in this process is largely unknown. Here, we investigated function cytoskeletal intermediate filament protein vimentin life cycle infection. Using advanced imaging techniques, uncovered that filaments undergo drastic reorganization upon viral synthesis form a perinuclear cage-like...
Near-infrared (NIR) light penetrates tissue deeply, but its application to motor behavior stimulation has been limited by the lack of known genetic NIR light-responsive sensors. We designed and synthesized a Yb3+/Er3+/Ca2+-based lanthanide-doped upconversion nanoparticle (UCNP) that effectively converts 808 nm green emission. This UCNP is compatible with Chrimson, cation channel activated light; as such, it can be used in optogenetic manipulation behaviors Caenorhabditis elegans. show this...
Activation of NLRP3 inflammasome is precisely controlled to avoid excessive activation. Although multiple molecules regulating activation have been revealed, the checkpoints governing remain elusive. Here, we show that governed by GSTO1-promoted ASC deglutathionylation in macrophages. Glutathionylation inhibits oligomerization and thus represses macrophages, unless GSTO1 binds deglutathionylates at ER, under control mitochondrial ROS triacylglyceride synthesis. In macrophages expressing...
Visualization of specific genomic loci in live cells is a prerequisite for the investigation dynamic changes chromatin architecture during diverse biological processes, such as cellular aging. However, current precision imaging methods are hampered by lack fluorescent probes with high specificity and signal-to-noise contrast. We find that conventional transcription activator-like effectors (TALEs) tend to form protein aggregates, thereby compromising their performance applications. Through...
Silencing resistance genes of pathogenic bacteria by RNA interference (RNAi) is a potential strategy to fight antibiotic-resistant bacterial infections. Currently, RNAi cannot be achieved in due the lack RNA-induced silencing complex machinery and difficulty small interfering (siRNA) delivery. Here, we show that exosomal siRNAs can efficiently delivered into cells silence target primarily through translational repression without mRNA degradation. The Argonaute 2 (AGO2) protein forms with...
Secretory granules, also known as dense core vesicles, are generated at the trans-Golgi network and undergo several maturation steps, including homotypic fusion of immature secretory granules (ISGs) processing prehormones to yield active peptides. The molecular mechanisms governing granule largely unknown. Here, we investigate a highly conserved protein named HID-1 in mouse model. A conditional knockout pancreatic β cells leads glucose intolerance remarkable increase serum proinsulin/insulin...
Doxorubicin is a widely used chemotherapeutic agent for the treatment of variety solid tumors.However, resistance to this anticancer drug major obstacle effective tumors.As mitochondria play important roles in cell life and death, we anticipate that may be related resistance.Here, stable isotope labeling by amino acids culture (SILAC)-based quantitative proteomic strategy was applied compare mitochondrial protein expression doxorubicin sensitive OVCAR8 cells its doxorubicin-resistant variant...
Reactive oxygen species can induce cell apoptosis, and oxidative stress has been implicated in a variety of neurodegenerative disorders. Tomatine, which is naturally occurring steroidal glycoalkaloid isolated from Solanum cathayanum, shown potent anti-oxidant properties.In this study, we used the SH-SY5Y line as an vitro model investigated protective effect tomatine against hydrogen peroxide (H2 O2 )-induced neurotoxicity cells.Tomatine might inhibit release cellular lactate dehydrogenase,...
Insulin levels are essential for the maintenance of glucose homeostasis, and deviations lead to pathoglycemia or diabetes. However, metabolic mechanism controlling insulin quantity quality is poorly understood. In pancreatic β cells, homeostasis release tightly governed by secretory granule (ISG) trafficking, but required regulators mechanisms largely unknown. Here, we identified that VAMP4 controlled in response challenge. deficiency led increased blood hyperresponsiveness glucose. packaged...
The existence of clathrin‐independent recycling secretory vesicles has been controversial. By combining patch‐clamp capacitance recording, optical methods and specific molecular interventions, we dissect two types mechanistically different endocytosis in pancreatic β cells, both which require GTP dynamin. fast one is a novel but actin‐dependent that triggered by high cytoplasmic Ca 2+ concentration ([Ca ] i ). Large fluorescent dextran (10 nm diameter) was able to be internalized this...