A5101773783- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Telomeres, Telomerase, and Senescence
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- Parkinson's Disease Mechanisms and Treatments
- Asthma and respiratory diseases
- Polymer Surface Interaction Studies
- Antimicrobial agents and applications
- Cancer-related molecular mechanisms research
- Endoplasmic Reticulum Stress and Disease
- Inhalation and Respiratory Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Ubiquitin and proteasome pathways
- Pain Mechanisms and Treatments
- Gout, Hyperuricemia, Uric Acid
- Veterinary Equine Medical Research
- Heme Oxygenase-1 and Carbon Monoxide
- Muscle activation and electromyography studies
ShanghaiTech University
2025
Jinan University
2023-2024
Zhengzhou University
2024
Carnegie Mellon University
2021-2024
The University of Texas MD Anderson Cancer Center
2022-2024
Henan Provincial People's Hospital
2024
Zhengzhou People's Hospital
2024
First Affiliated Hospital of Jinan University
2024
Columbia University Irving Medical Center
2021-2023
Jilin University
2023
Abstract CRISPR/Cas13 systems are increasingly used for programmable targeting of RNAs. While Cas13 nucleases capable degrading both target RNAs and bystander in vitro bacteria, initial studies fail to detect collateral degradation non-target eukaryotic cells. Here we show that RfxCas13d, also known as CasRx, a widely system, can cause transcriptome destruction when abundant reporter RNA endogenous RNAs, resulting proliferation defect these results call caution using RfxCas13d targeted...
Mutations or inactivation of parkin, an E3 ubiquitin ligase, are associated with familial form sporadic Parkinson's disease (PD), respectively, which manifested the selective vulnerability neuronal cells in substantia nigra (SN) and striatum (STR) regions. However, underlying molecular mechanism linking parkin etiology PD remains elusive. Here we report that p62, a critical regulator for protein quality control, inclusion body formation, autophagy diverse signaling pathways, is new substrate...
Currently, undeveloped diagnosis and delayed treatment of bacteria-infected sites in vivo not only expand the risk tissue infection but are also a major clinical cause multiple drug-resistant bacterial infections. Herein, an efficient nanoplatform for near-infrared (NIR)-light-controlled release bacteria-targeted delivery nitric oxide (NO) combined with photothermal therapy (PTT) is presented. Using maltotriose-decorated mesoporous polydopamine (MPDA-Mal) BNN6, smart antibacterial...
Abstract Bacterial infections, especially those from drug‐resistant strains, pose a significant threat to healing diabetic skin injuries, with current treatments being intricated and often unsatisfactory. Inspired by octopuses, biomimetic material using α‐cyclodextrin (α‐CD) polyethylene glycol (PEG) assembled graphene oxide end‐capped polyrotaxanes (GO‐PR) is developed, where α‐CD mimics the flexible tentacles of an octopus. Further, cationically modified polyethyleneimine (PEI) resemble...
Mitophagy is responsible for removal of damaged mitochondria and therefore a fundamental process in mitochondrial quality control. Both ubiquitin-dependent receptor-dependent pathways are considered to mediate mitophagy. These distinct mechanisms may be activated response stresses. An intriguing question whether how crosstalk occurs between the coordinate We have uncovered striking piece evidence demonstrate that mitophagy receptor FUNDC1 substrate MARCH5, mitochondrially localized E3...
Abstract Chromosomal rearrangements, such as translocations, deletions, and inversions, underlie numerous genetic diseases cancers, yet precise engineering of these rearrangements remains challenging. Here, we present a CRISPR-based homologous recombination-mediated rearrangement (HRMR) strategy that leverages donor templates to align repair broken chromosome ends. HRMR improves efficiency by approximately 80-fold compared non-homologous end joining, achieving over 95% recombination....
Introduction: Transcriptional regulation is an important process wherein non-protein coding enhancer sequences play a key role in determining cell type identity and phenotypic diversity. In neural tissue, these gene regulatory processes are crucial for coordinating plethora of interconnected regionally specialized types, ensuring their synchronized activity generating behavior. Recognizing the intricate interplay brain imperative, as mounting evidence links neurodevelopment neurological...
Alcohol abuse has recently become a serious health concern worldwide, and the incidence of alcoholic liver disease (ALD) is rapidly increasing with high morbidity mortality. Ferroptosis newly recognized form regulated cell death caused by iron-dependent accumulation lipid peroxidation. Here we showed that circadian clock protein BMAL1 in hepatocytes both necessary sufficient to protect against ALD mitigating ferroptosis. Upon exposure alcohol (5 % Lieber-DeCarli liquid diet for 10 days...
ABSTRACT While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack tools that are able target specific cells based on these markers. Here we show targeting a single marker with CRISPR/RfxCas13d led collateral transcriptome destruction in human cells, which can be harnessed inhibit proliferation suppress state transition.
TERRA, TElomeric Repeat-containing RNA, is a long non-coding RNA transcribed from telomeres. Emerging evidence indicates that TERRA regulates telomere maintenance and chromosome end protection in normal cancerous cells. However, the mechanism of how contributes to functions still unclear, partially owing shortage approaches track manipulate endogenous molecules live Here, we developed method visualize cells via combination CRISPR Cas13 labeling SunTag technology. Single-particle tracking...
The cochlear implant (CI), an advanced electronic device replacing the entire function, is ultimate treatment for over 466 million people with disabling hearing loss. Infection after implantation a common and worrisome complication despite routine administration of antibiotic. bacterial biofilms formed on surface CI are main cause antibiotic failure. To solve this problem, we developed copper-containing zwitterionic coating consisting anti-adherent poly sulfobetaine methacrylate (PSB)...
Abstract Background Smoking induces and modifies the airway immune response, accelerating decline of asthmatics’ lung function severely affecting asthma symptoms’ control level. To assess prognosis asthmatics who smoke to provide reasonable recommendations for treatment, we constructed a nomogram prediction model. Methods General clinical data were collected from April September 2021 smoking aged ≥14 years attending People’s Hospital Zhengzhou University. Patients followed up regularly by...
ABSTRACT Translation is pervasive outside of canonical coding regions, occurring in lncRNAs, UTRs, and introns. While the resulting polypeptides are often non-functional, translation noncoding regions nonetheless necessary for birth new regions. The mechanisms underlying surveillance diverse how escaped evolve functions remain unclear. Intriguingly, sequence-derived functional peptides localize to membranes. Here, we show that intrinsic nucleotide bias genome genetic code frequently results...
Summary Key mechanisms underlying chronic pain occur within the neural circuitry of dorsal horn. Recent genome-wide association studies (GWAS) have identified genetic variants associated with predisposition to pain. However, most these lie in regulatory non-coding regions that so far not been linked spinal cord function. Here, we take a multi-species approach determine whether impact elements horn neurons. We first built more comprehensive single cell atlas; filling gaps by generating...
Abstract Genetic studies are rapidly identifying non-protein-coding human disease-associated loci. Understanding the regulatory mechanisms underlying these loci remains a challenge because causal variants and tissues in which they act often unclear. Massively parallel reporter assays (MPRAs) have potential to link differences genome sequence, including genetic variants, tissue-specific function. Although MPRA similar technologies been widely adopted cell culture, there several barriers...
Abstract Renal medullary carcinoma (RMC) is a rare but highly aggressive SMARCB1-deficient kidney cancer that mainly afflicts young individuals of African descent and has few treatment options. Despite the advancements in immune checkpoint therapy (ICT) for other cancers, its efficacy against RMC remains elusive. The majority patients treated with ICT have experienced aggressively progressive disease as best response. This hyperprogression was confirmed our prospective clinical trial...
Alzheimer’s disease (AD) involves aggregation of amyloid β and tau, neuron loss, cognitive decline, neuroinflammatory responses. Both resident microglia peripheral immune cells have been associated with the component AD. However, relative contribution cell types to AD predisposition has not thoroughly explored due their similarity in gene expression function. To study effects AD-associated variants on cis -regulatory elements, we train convolutional neural network (CNN) regression models...
This study aimed to identify environmental risk factors associated with asthmatic fixed airflow obstruction (FAO) and assess the relationship between small airway abnormalities defined by forced expiratory flow at 25-75% (FEF25-75%) FAO.