A5084577935- Cancer Research and Treatments
- Bone and Dental Protein Studies
- Myasthenia Gravis and Thymoma
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Bacterial Identification and Susceptibility Testing
- Digestive system and related health
- Prion Diseases and Protein Misfolding
- Pneumonia and Respiratory Infections
- Lung Cancer Treatments and Mutations
- Antibiotic Resistance in Bacteria
- Virus-based gene therapy research
- Mycobacterium research and diagnosis
- Renal and related cancers
- Lung Cancer Research Studies
- Nuclear Structure and Function
- Cancer therapeutics and mechanisms
- Tuberculosis Research and Epidemiology
- Pituitary Gland Disorders and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Antibiotic Use and Resistance
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
The People's Hospital of Guangxi Zhuang Autonomous Region
2024
Guangxi Zhuang Autonomous Region Health and Family Planning
2024
Novogene Bioinformatics Institute
2017-2020
Georgetown University
2015-2020
Georgetown University Medical Center
2015-2020
The First Affiliated Hospital, Sun Yat-sen University
2017
Sun Yat-sen University
2017
Nankai University
2012-2016
State Key Laboratory of Medicinal Chemical Biology
2016
Huazhong University of Science and Technology
2013
Abstract Purpose: CD44 is of functional importance for tumor initiation and progression in colorectal cancer, but how this molecule benefits cancer cells from the microenvironment, especially tumor-associated macrophages (TAM), remains poorly defined. Experimental Design: In vivo tumorigenic assays were conducted to assess role murine TAMs tumorigenesis human cells. Both vitro osteopontin (OPN) expression levels examined by immunohistochemistry, quantitative PCR, Western blotting. Soft agar...
Mutations or inactivation of parkin, an E3 ubiquitin ligase, are associated with familial form sporadic Parkinson's disease (PD), respectively, which manifested the selective vulnerability neuronal cells in substantia nigra (SN) and striatum (STR) regions. However, underlying molecular mechanism linking parkin etiology PD remains elusive. Here we report that p62, a critical regulator for protein quality control, inclusion body formation, autophagy diverse signaling pathways, is new substrate...
Multidrug-resistant (MDR) bacteria are important public health problems. Antibiotic susceptibility testing (AST) currently uses time-consuming culture-based procedures, which cause treatment delays and increased mortality. We developed a machine learning model using Acinetobacter baumannii as an example to explore fast AST approach metagenomic next-generation sequencing (mNGS) data. The key genetic characteristics associated with antimicrobial resistance (AMR) were selected through least...
Abstract Cancer stem cells are implicated in tumor progression, metastasis, and recurrence, although the exact mechanisms remain poorly understood. Here, we show that expression of cellular prion protein (PrPc, PRNP) is positively correlated with an increased risk metastasis colorectal cancer. PrPc defines a subpopulation CD44-positive cancer contributes to metastatic capacity. PrPc+CD44+ displayed high liver capability, unlike PrPc−CD44+ cells, was inhibited by RNAi-mediated attenuation...
Abstract Accumulating evidence supports a role of the PI3K-AKT pathway in regulation cell motility, invasion and metastasis. AKT activation is known to promote metastasis, however under certain circumstances, it also shows an inhibitory activity on metastatic processes, cause such conflicting results largely unclear. Here we found that AKT1 important regulator metastasis down-regulation its associated with increased potential A549 cells. Inhibition enhanced migration KRAS- or EGFR-mutant...
Chronic exposure to morphine can induce drug addiction and neural injury, but the exact mechanism is not fully understood. Here we show that induces autophagy in Neuroblastoma SH-SY5Y cells rat hippocampus. Pharmacological approach shows this effect appears be mediated by PTX-sensitive G protein-coupled receptors signaling cascade. Morphine increases Beclin 1 expression reduces interaction between Bcl-2, thus releasing for its pro-autophagic activity. Bcl-2 overexpression inhibits...
Overcoming tumor heterogeneity is a major challenge for personalized treatment of gastric cancer, especially human epidermal growth factor receptor‐2 targeted therapy. Analysis circulating DNA allows more comprehensive analysis than traditional biopsies in lung cancer and breast but little known cancer. We assessed mutation profiles ct primary tumors from 30 patients with advanced then performed mutations by multiple five patients, finally analyzed the concordance HER 2 amplification paired...
Amplicon-based next generation sequencing (NGS) approaches have been preferentially adopted by the clinical laboratories on basis of a short turnaround time (TAT) and small DNA input needs. However, little work has done to assess amplicon-based NGS methods for copy number variation (CNV) detection in comparison with current standard like immunohistochemistry (IHC) fluorescence situ hybridization (FISH). The correlation between based CNV later remained unexplored. We developed an panel detect...
Exportin 1 (XPO1) mediates nuclear export of many cellular factors known to play critical roles in malignant processes, and selinexor (KPT-330) is the first XPO1-selective inhibitor compound advanced clinical development phase for cancer treatment. We demonstrated here that inhibition XPO1 drives accumulation important cargo tumor suppressor proteins, including transcription factor FOXO3a p53 thymic epithelial (TET) cells, induces p53-dependent -independent antitumor activity vitro Selinexor...
Carbapenem resistance is a major concern in the management of antibiotic-resistant
The study aimed to develop a genotypic antimicrobial resistance testing method for Klebsiella pneumoniae using metagenomic sequencing data.
Metagenomic next-generation sequencing (mNGS) has greatly improved our understanding of pathogens in infectious diseases such as pulmonary tuberculosis (PTB). However, high human DNA background (> 95%) impedes the detection sensitivity mNGS identifying intracellular Mycobacterium (MTB), posing a pressing challenge for MTB diagnosis. Therefore, there is an urgent need to improve diagnosis performance PTB patients. In this study, we optimized method PTB. This led development host depletion...
ABSTRACT Antimicrobial resistance is an escalating global health crisis, underscoring the urgent need for timely and targeted therapies to ensure effective clinical treatment. We developed a machine learning model based on metagenomic next-generation sequencing (mNGS) rapid antimicrobial susceptibility prediction (mNGS-based AST), which was tailored five ESKAPEE bacteria: Acinetobacter baumannii , Klebsiella pneumoniae Escherichia coli Pseudomonas aeruginosa, Staphylococcus aureus . However,...
Since circulating tumor DNA (ctDNA) offers clear advantages as a minimally invasive method for monitoring compared with tissue, we aimed to evaluate genotyping ctDNA using next-generation sequencing- (NGS-) based panel identify the prognostic value of mutation status in metastatic colorectal cancer (mCRC) patients primary resected and subsequent lines treatment this study.76 mCRC treated Beijing Chao-Yang Hospital from 2011 2017 were enrolled. Genotyping RAS/BRAF tissue was determined by...