A5009546120- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Ferroptosis and cancer prognosis
- Phagocytosis and Immune Regulation
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Gastric Cancer Management and Outcomes
- Helicobacter pylori-related gastroenterology studies
- Colorectal Cancer Treatments and Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Epigenetics and DNA Methylation
- Effects of Radiation Exposure
- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Biochemical Analysis and Sensing Techniques
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Renal cell carcinoma treatment
- MicroRNA in disease regulation
- Renal and related cancers
- Lung Cancer Treatments and Mutations
Fujian University of Traditional Chinese Medicine
2019-2024
Fuzhou University
2021
University of Oklahoma Health Sciences Center
2011-2020
Coare Biotechnology (United States)
2018
University of Oklahoma
2013-2016
Oklahoma City University
2015-2016
Stem cell pluripotency, angiogenesis and epithelial-mesenchymal transition (EMT) have been shown to be significantly upregulated in pancreatic ductal adenocarcinoma (PDAC) many other aggressive cancers. The dysregulation of these processes is believed play key roles tumor initiation, progression, metastasis, contributory PDAC being the fourth leading cause cancer-related deaths US. suppressor miRNA miR-145 downregulates critical pluripotency factors oncogenes results repressed metastatic...
Doublecortin-like kinase 1 (DCLK1) is emerging as a tumor specific stem cell marker in colorectal and pancreatic cancer. Previous vitro vivo studies have demonstrated the therapeutic effects of inhibiting DCLK1 with small interfering RNA (siRNA) well genetically targeting DCLK1+ for deletion. However, activity not been studied directly. Therefore, we assessed using novel molecule inhibitor, LRRK2-IN-1, which demonstrates significant affinity DCLK1. Here report that LRRK2-IN-1 potent...
More than 80% of intestinal neoplasia is associated with the adenomatous polyposis coli (APC) mutation. Doublecortin-like kinase 1 (Dclk1), a protein, overexpressed in colorectal cancer and specifically marks tumor stem cells (TSCs) that self-renew increased progeny Apc Min/+ mice. However, role Dclk1 expression its contribution to regulating pro-survival signaling for progression mutant poorly understood.We analyzed DCLK1 gene datasets 329 specimens from TCGA Colon Adenocarcinoma Cancer...
Renal clear cell carcinoma (RCC) is the most common type of kidney cancer and 8th overall in US. RCC survival rates drop precipitously with regional distant spread recent studies have demonstrated that presents an epithelial-mesenchymal transition (EMT) phenotype linked to increased recurrence decreased survival. EMT a key characteristic tumor stem cells (TSCs) along chemo-resistance radio-resistance, which are also phenotypic RCC. Targeting these factors increasing patients....
Abstract Objectives Circadian rhythm controls complicated physiological activities in organisms. clock genes have been related to tumour progression, but its role glioma is unknown. Therefore, we explored the relationship between dysregulated circadian and progression. Materials Methods Samples were divided into different groups based on gene expression training dataset (n = 672) verified results other four validating datasets 1570). The GO GSEA enrichment analysis conducted explore...
The role of Dclk1(+) tuft cells in the replacement intestinal epithelia and reestablishing epithelial barrier after severe genotoxic insult is completely unknown. Successful restoration requires precise coordination between within each crypt subunit. While mechanisms that control this response remain largely uncertain, radiation model remains an exceptional surrogate for stem cell-associated loss. Following creation Dclk1-intestinal-epithelial-deficient Villin-Cre;Dclk1(flox/flox) mice,...
// Parthasarathy Chandrakesan 1 , 2 Nathaniel Weygant Randal May 4 Dongfeng Qu Harisha R. Chinthalapally Sripathi M. Sureban Naushad Ali Stan A. Lightfoot 3 Shahid Umar 5 Courtney W. Houchen Department of Medicine, University Oklahoma Health Sciences center, City, OK 73104, USA OU Cancer Institute, Pathology, Center, Veterans Affairs Medical Molecular and Integrative Physiology, Kansas KS, Correspondence to Houchen, M.D. e-mail: Courtney-houchen@ouhsc.edu Key words: Dclk1; self-renewal;...
Immunotherapy that has proven efficacy in several solid cancers plays a partial role improving clinical outcomes of advanced gastrointestinal (GI) cancers. There is an unmet need to find new immune-related therapeutic targets. Doublecortin-like kinase 1 (DCLK1) marks tuft cells which are recognized as cancer-initiating and regulators the type II immune response, been studied for its many including colon gastric cancers, but tumor immunity remains unexplored. In current study, we analyzed...
Renal cell carcinoma (RCC) is a common and devastating disease characterized by hypoxic microenvironment, epithelial‐mesenchymal transition potent resistance to therapy evidencing the presence of cancer stem cells (CSCs). Various CSC markers have been studied in RCC, but overall there limited data on their role most relatively nonspecific. Doublecortin‐like kinase 1 (DCLK1) validated marker gastrointestinal tract evidence for an equivalent other cancers accumulating. We used bioinformatics,...
In Barrett's esophagus (BE), the normal esophageal squamous epithelium is replaced with a specialized metaplastic columnar epithelium. BE premalignant lesion that can progress to adenocarcinoma (EAC). Currently, there are no early molecular indicators would predict progression from EAC. As only permanent residents of epithelium, stem cells have been implicated in this progression. The aim present study was determine expression doublecortin and CaM kinase-like-1 (DCAMKL-1) other putative...
Doublecortin-like kinase 1 (Dclk1), a microtubule-associated kinase, marks the fifth lineage of intestinal epithelial cells called tuft that function as tumor stem in Apc mutant models colon cancer. In order to determine role Dclk1 dextran sulfate sodium (DSS) induced colonic inflammation both specific deficient (VillinCre;Dclk1f/f) and control (Dclk1f/f) mice were fed 3% DSS drinking water for 9 days, allowed recover 2 killed. The clinical histological features DSS-induced colitis scored...
Doublecortin-like kinase 1 (DCLK1) is a putative pancreatic stem cell marker and upregulated in cancer, colorectal many other solid tumors. It marks tumor cells mouse models of intestinal neoplasia. Here we sought to determine whether DCLK1 protein can be detected the bloodstream if its levels archived serum samples could quantitatively assessed cancer patients. specific ELISA, western blotting, immunohistochemical analyses were used expression staining intensity tissues ductal...
Tumor stem cell marker Doublecortin-like kinase1 (DCLK1) is upregulated in several solid tumors. The role of DCLK1 hepatocellular carcinoma (HCC) unclear. We immunostained tissues from human livers with HCC, cirrhosis controls (CC), and non-cirrhosis (NCC) for DCLK1. Western blot ELISA analyses were performed stored plasma samples. observed increased immunoreactive epithelia stroma HCC CCs compared NCCs, a marked increase patients CC NCC. Analysis the Cancer Genome Atlas' dataset revealed...
Oncogenic KRAS mutation plays a key role in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis with nearly 95% of PDAC harboring mutation-activated KRAS, which has been considered an undruggable target. Doublecortin-like kinase 1 (DCLK1) is often overexpressed cancer, and recent studies indicate that DCLK1+ cells can initiate tumorigenesis. In this study, we investigate whether overexpressing DCLK1 activates RAS promotes tumorigenesis, metastasis, drug resistance. Human cancer (AsPC-1...
// Parthasarathy Chandrakesan 1, 2 , Randal May 3 Dongfeng Qu Nathaniel Weygant 1 Vivian E. Taylor James D. Li Naushad Ali Sripathi M. Sureban Michael Qante 4 Timothy C. Wang 5 S. Bronze Courtney W. Houchen 2, 3, 6 Department of Medicine, University Oklahoma Health Sciences Center, City, OK, USA Stephenson Cancer Veterans Affairs Medical Klinikum rechts der Isar, II. Medizinische Klinik, Technische Universität München, Munich, Germany Digestive and Liver Diseases, Columbia New York, NY,...
Abstract Crypt epithelial survival and regeneration after injury require highly coordinated complex interplay between resident stem cells diverse cell types. The function of Dclk1 expressing tuft regulating intestinal DNA damage response for survival/self-renewal radiation-induced is unclear. Intestinal (IECs) were isolated purified utilized experimental analysis. We found that small crypts Villin Cre ;Dclk1 f/f mice hypoplastic more apoptotic 24 h post-total body irradiation, a time when...
Chemoresistance cells have features similar to cancer stem cells. Elimination of these is an effective therapeutic strategy clinically combat chemoresistance non-small cell lung (NSCLC). Here, we demonstrate that Doublecortin-like kinase1 (DCLK1) the key developing and associated stemness in NSCLC. DCLK1 highly expressed human adenocarcinoma strongly correlated with stemness. Silencing inhibits NSCLC primary secondary spheroid formation, which prerequisite feature tumor inhibition reduced...
Doublecortin-like kinase 1 (DCLK1) is a gastrointestinal (GI) tuft cell that has been investigated as biomarker of cancer stem-like cells in colon and pancreatic cancers. However, its utility may be limited principle by signal instability dilution heterogeneous tumors, where the proliferation diverse tumor lineages obscures direct measurement DCLK1 activity. To address this issue, we explored definition miRNA signature surrogate for cells. Utilizing RNA/miRNA-sequencing datasets from Cancer...
Since circulating tumor DNA (ctDNA) offers clear advantages as a minimally invasive method for monitoring compared with tissue, we aimed to evaluate genotyping ctDNA using next-generation sequencing- (NGS-) based panel identify the prognostic value of mutation status in metastatic colorectal cancer (mCRC) patients primary resected and subsequent lines treatment this study.76 mCRC treated Beijing Chao-Yang Hospital from 2011 2017 were enrolled. Genotyping RAS/BRAF tissue was determined by...
Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related deaths worldwide. Chronic hepatitis C virus (HCV) infection causes induction several tumors/cancer stem cell (CSC) markers and known to be a major risk factor for development HCC. Therefore, drugs that simultaneously target viral replication CSC properties are needed risk-free treatment advanced stage liver diseases, including Here, we demonstrated (Z)-3,5,4'-trimethoxystilbene (Z-TMS) exhibits potent antitumor...
The approval of immune checkpoint inhibitors has expanded treatment options for renal cell carcinoma (RCC), but new therapies that target RCC stemness and promote anti-tumor immunity are needed. Previous findings demonstrate doublecortin-like kinase 1 (DCLK1) regulates is associated with disease progression. Herein, we small-molecule inhibitor DCLK1-IN-1 strongly inhibits DCLK1 phosphorylation downregulates pluripotency factors cancer stem (CSC) or epithelial-mesenchymal transition...