A5022746981- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Marine Sponges and Natural Products
- Marine Invertebrate Physiology and Ecology
- RNA Interference and Gene Delivery
- Aquaculture disease management and microbiota
- Galectins and Cancer Biology
- Circular RNAs in diseases
Columbia University Irving Medical Center
2021-2023
Abstract CRISPR/Cas13 systems are increasingly used for programmable targeting of RNAs. While Cas13 nucleases capable degrading both target RNAs and bystander in vitro bacteria, initial studies fail to detect collateral degradation non-target eukaryotic cells. Here we show that RfxCas13d, also known as CasRx, a widely system, can cause transcriptome destruction when abundant reporter RNA endogenous RNAs, resulting proliferation defect these results call caution using RfxCas13d targeted...
ABSTRACT While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack tools that are able target specific cells based on these markers. Here we show targeting a single marker with CRISPR/RfxCas13d led collateral transcriptome destruction in human cells, which can be harnessed inhibit proliferation suppress state transition.
ABSTRACT Translation is pervasive outside of canonical coding regions, occurring in lncRNAs, UTRs, and introns. While the resulting polypeptides are often non-functional, translation noncoding regions nonetheless necessary for birth new regions. The mechanisms underlying surveillance diverse how escaped evolve functions remain unclear. Intriguingly, sequence-derived functional peptides localize to membranes. Here, we show that intrinsic nucleotide bias genome genetic code frequently results...
NF-κB is an evolutionarily conserved eukaryotic transcription factor that plays a role in many important developmental and immune-related processes by activating target gene expression. The goal of these experiments was to define the sequences required for sea anemone NF-κB's intrinsic transactivation activity using mutant proteins with serial deletions N- C-terminal sequences. Deletion mutants were constructed missing 15, 32 or 47 amino acids (aa) N-terminal 17, 27 aa 440 protein from...