A5078228514- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Phytase and its Applications
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Polysaccharides and Plant Cell Walls
- RNA modifications and cancer
- Genetic diversity and population structure
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Enzyme Catalysis and Immobilization
- Proteoglycans and glycosaminoglycans research
- Carbohydrate Chemistry and Synthesis
- Digestive system and related health
- Computational Drug Discovery Methods
- Infant Nutrition and Health
- Lepidoptera: Biology and Taxonomy
- Synthesis and biological activity
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
State Key Laboratory of Microbial Technology
2011-2025
Shandong University
2011-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Nanjing University
2025
Nanjing Drum Tower Hospital
2025
Nantong University
2023-2024
Qingdao Binhai University
2019-2024
Jining Normal University
2024
Weifang People's Hospital
2019-2023
Facing Our Risk of Cancer Empowered
2021
The diamondback moth Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) is one of the most destructive insect pests cruciferous plants worldwide. Biological, ecological and genetic studies have indicated that this migratory in many regions around world. Although outbreaks pest occur annually China cause heavy damage, little known concerning its migration. To better understand migration pattern, we investigated population structure demographic history by analyzing 27 geographical...
α-Glucanotransferases of the CAZy family GH70 convert starch-derived donors to industrially important α-glucans. Here, we describe characteristics a novel GtfB-type 4,6-α-glucanotransferase high enzyme activity (60.8 U mg
(+)-Nootkatone is a highly valued sesquiterpenoid compound, exhibiting typical grapefruit aroma and various desired biological activities for use as aromatics pharmaceuticals. The high commercial demand of (+)-nootkatone predominately met by chemical synthesis, which entails the environmentally harmful reagents. Efficient synthesis via biotechnological approaches thus urgently needed to satisfy its industrial demand. However, there are only limited number studies that report de novo from...
Abstract Background The sesquiterpene germacrene A is a direct precursor of ß-elemene that major component the Chinese medicinal herb Curcuma wenyujin with prominent antitumor activity. microbial platform for production was previously established in Saccharomyces cerevisiae using synthase (LTC2) Lactuca sativa . Results We evaluated performance LTC2 ( Ls GAS) as well nine other identified or putative synthases from different sources A. Av GAS, Anabaena variabilis , found to be most efficient...
The saprophytic filamentous fungus Trichoderma reesei represents one of the most prolific cellulase producers and also has potential to be developed into a tractable fungal host for biosynthesizing secondary metabolite products. To expedite genetic engineering fungi, efficient DNA assembly processes that can facilitate transfer large-sized hosts, including T. reesei, are still in demand. Here, we method simultaneous vivo targeted genome integration multiple fragments (SATIMD) reesei. While...
Silencing resistance genes of pathogenic bacteria by RNA interference (RNAi) is a potential strategy to fight antibiotic-resistant bacterial infections. Currently, RNAi cannot be achieved in due the lack RNA-induced silencing complex machinery and difficulty small interfering (siRNA) delivery. Here, we show that exosomal siRNAs can efficiently delivered into cells silence target primarily through translational repression without mRNA degradation. The Argonaute 2 (AGO2) protein forms with...
Abstract Chilo suppressalis (Walker) displays significant geographical differences in ecological preference that may be congruent with patterns of molecular variation. To test this, we collected and analysed 381 individuals this species from cultivated rice at 18 localities China during the rice‐growing season 2005–2006. We used four microsatellite DNA markers mitochondrial gene fragments. found is highly differentiated, coupled an estimated population expansion date least 60 000 bp ....
Summary Cellulase gene expression in Trichoderma reesei is highly responsive to environmental cues and under stringent regulation by multiple transcription factors. XYR1 (Xylanase regulator 1) has been identified as the most important transcriptional activator of cellulase/hemicellulase although precise transactivating mechanism remains largely elusive. Here we show that activation domain interacts with T . homolog TrSNF12 subunit SWI/SNF complex. Deletion Trsnf12 markedly impaired induced...
The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is widely used as a cellulase producer in the industry. Herein, we describe rational engineering of publicly available T. QM9414 strain to achieve remarkable high-level production on glucose. Overexpression key regulator XYR1 by copper-repressible promoter Ptcu1 was first implemented full context catabolite repression (CCR) while eliminating requirement inducing sugars for enzyme production. bgl1 gene further...
Engineering dynamic control of gene expression is desirable because many engineered functions interfere with endogenous cellular processes that have evolved to facilitate growth and survival. Minimizing conflict between production phases can therefore improve product titers in microbial cell factories. We developed an autoinduced system by rewiring the Saccharomyces cerevisiae pheromone response pathway. To ameliorate reduction due early onset at low population densities, α-pheromone...
To achieve cost-effective production of lignocellulolytic enzymes for biorefinery processes, engineering transcription factors represents a powerful strategy to boost cellulase and xylanase in Trichoderma reesei. In this study, novel mutation (R434L) regulator 1 (Xyr1) was identified based on the yeast one-hybrid screening system. The point located middle homology region Xyr1 with unclear functions, indicating significant role domain tuning transactivation. When constitutively expressed T....
The probiotic bacterium Lactobacillus reuteri 121 produces a complex, branched (1 → 4, 1 6)-α-d-glucan as extracellular polysaccharide (reuteran) from sucrose (Suc), using single glucansucrase/glucosyltransferase (GTFA) enzyme (reuteransucrase). To gain insight into the reaction/product specificity of GTFA and mechanism reuteran formation, incubations with Suc and/or series malto-oligosaccharides (MOSs) (degree polymerization (DP2–DP6)) were followed in time. structures initially formed...
Microbial β-galactosidase enzymes are widely used as biocatalysts in industry to produce prebiotic galactooligosaccharides (GOS) from lactose. GOS mixtures beneficial additives infant formula mimic the effects of human milk oligosaccharides (hMOS). The structural variety is significantly lower than hMOS. Since this complexity considered basis for multiple biological functions hMOS, it important broaden structures. In study, residue R484 near +1 subsite C-terminally truncated Bacillus...
Highly conserved glycoside hydrolase family 70 glucansucrases are able to catalyze the synthesis of α-glucans with different structure from sucrose. The structural determinants glucansucrase specificity have remained unclear. Residue Leu(940) in domain B GTF180, probiotic bacterium Lactobacillus reuteri 180, was shown vary and is close +1 glucosyl unit crystal GTF180-ΔN complex maltose. Herein, we show that mutations wild-type all caused an increased percentage (α1→6) linkages a decreased...
Abstract The Glycoside hydrolase (GH) family 70 originally was established for glucansucrases of lactic acid bacteria (LAB) converting sucrose into α-glucan polymers. In recent years we have identified 3 subfamilies GH70 enzymes (designated GtfB, GtfC and GtfD) as 4,6-α-glucanotransferases, cleaving (α1 → 4)-linkages in maltodextrins/starch synthesizing new 6)-linkages. this work, 106 putative GtfBs were the Nestlé Culture Collection genome database with ~2700 genomes, L . reuteri NCC 2613...
Nine GtfB-like 4,6-α-glucanotransferases (4,6-α-GTs) (represented by GtfX of L. aviarius subsp. DSM 20655) were identified to show distinct characteristics in conserved motifs I-IV. In particular, the "fingerprint" Tyr motif III these nine GtfB-type 4,6-α-GTs was found be replaced a Trp. DSM20655, second protein (GtfY), containing canonical GtfB residue III, located directly upstream GtfX. Biochemical characterization revealed that both and GtfY showed 4,6-α-GT activity, cleaving (α1→4)...
α-Glucans produced by glucansucrase enzymes hold strong potential for industrial applications. The exact determinants of the linkage specificity have remained largely unknown, even with recent elucidation crystal structures. Guided structure GTF180-ΔN from Lactobacillus reuteri 180 in complex acceptor substrate maltose, we identified several residues (Asp-1028 and Asn-1029 domain A, as well Leu-938, Ala-978, Leu-981 B) near subsite +1 that may be critical determination, investigated these...