A5100444170- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Enzyme Catalysis and Immobilization
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Polysaccharides and Plant Cell Walls
- DNA Repair Mechanisms
- Microbial Natural Products and Biosynthesis
- Microbial Metabolites in Food Biotechnology
- Enzyme-mediated dye degradation
- Immune Response and Inflammation
- RNA Research and Splicing
- Cancer Research and Treatments
- Probiotics and Fermented Foods
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Advanced Cellulose Research Studies
- Gut microbiota and health
- Geomagnetism and Paleomagnetism Studies
- Endoplasmic Reticulum Stress and Disease
State Key Laboratory of Microbial Technology
2016-2025
Shandong University
2016-2025
Southern Medical University
2021-2025
Nanfang Hospital
2021-2025
Guangdong Academy of Agricultural Sciences
2024
Qingdao Binhai University
2019-2024
Beijing Forestry University
2023
Albert Einstein College of Medicine
2014-2022
Qingdao University of Science and Technology
2022
Chinese Academy of Sciences
2015-2021
Intestinal ischemia/reperfusion (I/R) injury has high morbidity and mortality rates. Gut microbiota is a potential key factor affecting intestinal I/R injury. Populations exhibit different sensitivities to injury; however, whether this interpopulation difference related variation in gut unclear. Here, elucidate the interaction between microbiome injury, we performed 16S DNA sequencing on preoperative feces of C57BL/6 mice fecal transplantation (FMT) experiments germ-free mice. The transwell...
The development of a controllable reactive nitrogen species (RNS) generation system for cancer treatment has remained elusive. Herein, supramolecular prodrug nanoassemblies (SPNA) strategy that co-delivers nitric oxide (NO) donor and superoxide anion (O2•–) inducing chemotherapeutic agent was reported RNS-potentiated chemotherapy. mole ratio platinum(IV) NO could be precisely tailored in SPNAPt/NO. Platinum(II) would released intracellularly to produce highly toxic RNS, peroxynitrite...
Proper perception of the extracellular insoluble cellulose is key to initiating rapid synthesis cellulases by cellulolytic Trichoderma reesei. Uptake soluble oligosaccharides derived from hydrolysis represents a potential point control in induced cascade. In this study, we identified major facilitator superfamily sugar transporter Stp1 capable transporting cellobiose reconstructing assimilation system Saccharomyces cerevisiae. The absence T. reesei resulted differential response Avicel...
Long-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases hemicellulases from Penicillium decumbens 114 which was isolated 30 years ago. Here, the genome P. JU-A10-T sequenced compared with that wild-type 114-2. Further, transcriptomes secretomes were between strains. Selective hyper-production but not all secreted proteins observed in mutant, making it more specific producer lignocellulolytic enzymes. Functional analysis...
Summary Cellulase gene expression in the model cellulolytic fungus Trichoderma reesei is supposed to be controlled by an intricate regulatory network involving multiple transcription factors. Here, we identified a novel transcriptional repressor of cellulase expression, Rce1. Disruption rce1 not only facilitated induced genes but also led significant delay terminating induction process. However, Rce1 did participate Cre1‐mediated catabolite repression. Electrophoretic mobility shift (EMSA)...
Trichoderma reesei represents an important workhorse for industrial production of cellulases as well other proteins. The large-scale is usually performed in a substrate-inducing manner achieved by fine-tuned cooperation suite transcription factors. Their and subsequent analysis are, however, often either difficult to manipulate or complicated the concomitant inducible Alternatives control gene expression independent nutritional state are thus preferred some cases facilitate not only...
ABSTRACT Appropriate perception of cellulose outside the cell by transforming it into an intracellular signal ensures rapid production cellulases cellulolytic Hypocrea jecorina . The major extracellular β-glucosidase BglI (CEL3a) has been shown to contribute efficient induction cellulase genes. Multiple β-glucosidases belonging glycosyl hydrolase (GH) family 3 and 1, however, exist in H. Here we demonstrated that CEL1b, like CEL1a, was displaying vitro transglycosylation activity. We then...
Cell death is an important biological process that believed to have a central role in intestinal ischaemia/reperfusion (I/R) injury. While the apoptosis inhibition pivotal preventing I/R, how necrotic cell regulated remains unknown. Necroptosis represents newly discovered form of programmed combines features both and necrosis, it has been implicated development range inflammatory diseases. Here, we show receptor-interacting protein 1/3 (RIP1/3) kinase mixed lineage domain-like recruitment...
The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in cerevisiae[1]. This data article contains transport characteristics of S. fluorescence fusion protein Mgt05196p-GFP expressing strain located on cell surface demonstrated that targeted to plasma membrane hxt null cerevisiae endowed with glucose d-xylose absorption capacity, as well native Gal2p. transmembrane...
(+)-Nootkatone is a highly valued sesquiterpenoid compound, exhibiting typical grapefruit aroma and various desired biological activities for use as aromatics pharmaceuticals. The high commercial demand of (+)-nootkatone predominately met by chemical synthesis, which entails the environmentally harmful reagents. Efficient synthesis via biotechnological approaches thus urgently needed to satisfy its industrial demand. However, there are only limited number studies that report de novo from...
The CRISPR/Cas9 technology is being employed as a convenient tool for genetic engineering of the industrially important filamentous fungus Trichoderma reesei. However, multiplex gene editing still constrained by sgRNA processing capability, hindering strain improvement T. reesei production lignocellulose-degrading enzymes and recombinant proteins.
Saprotrophy on plant biomass is a recently developed nutrition strategy for Trichoderma. However, the physiology and evolution of this new still elusive. We report deep sequencing analysis genome Trichoderma longibrachiatum, an efficient cellulase producer. The 31.7-Mb genome, smallest among sequenced species, encodes fewer nutrition-related genes than saprotrophic T. reesei (Tr), including glycoside hydrolases nonribosomal peptide synthetase–polyketide synthase. Homology phylogenetic...