A5015947223- Advanced Fluorescence Microscopy Techniques
- Laser-Matter Interactions and Applications
- Advanced Fiber Laser Technologies
- Cell Image Analysis Techniques
- Photonic Crystal and Fiber Optics
- Image Processing Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Diabetes and associated disorders
- Photoacoustic and Ultrasonic Imaging
- Pancreatic function and diabetes
- Optical Coherence Tomography Applications
- Congenital heart defects research
- Diabetes Management and Research
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- Microtubule and mitosis dynamics
- Neuroendocrine regulation and behavior
- Nonlinear Optical Materials Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Angiogenesis and VEGF in Cancer
- Nanofabrication and Lithography Techniques
- Digital Holography and Microscopy
- Zebrafish Biomedical Research Applications
Norwegian University of Science and Technology
2020-2022
Center for Life Sciences
2014-2021
Peking University
2010-2021
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2014-2021
Kavli Institute for Systems Neuroscience
2021
State Key Laboratory of Natural and Biomimetic Drugs
2020
Beijing National Laboratory for Molecular Sciences
2015-2018
Biotechnology Institute
2014-2015
Institute of Molecular Medicine
2014-2015
National Center for Nanoscience and Technology
2015
We developed a miniaturized two-photon microscope (MINI2P) for fast, high-resolution, multiplane calcium imaging of over 1,000 neurons at time in freely moving mice. With weight below 3 g and highly flexible connection cable, MINI2P allowed stable with no impediment behavior variety assays compared to untethered, unimplanted animals. The improved cell yield was achieved through optical system design featuring an enlarged field view (FOV) microtunable lens increased z-scanning range speed...
We report 37.4 fs pulse generation from a ring-cavity Er:fiber laser at repetition rate of 225 MHz. The spectral bandwidth the is 135 nm. single-pulse energy 0.31 nJ.
The medial entorhinal cortex (MEC) creates a map of local space, based on the firing patterns grid, head-direction (HD), border, and object-vector (OV) cells. How these cell types are organized anatomically is debated. In-depth analysis this question requires collection precise anatomical activity data across large populations neurons during unrestrained behavior, which neither electrophysiological nor previous imaging methods fully afford. Here, we examined topographic arrangement spatially...
Conventional 2D structured illumination microscopy (SIM) requires nine raw images to reconstruct a super-resolved image. In order increase the frame rate of SIM, attempts are being made reduce number SIM images. However, all proposed reconstruction algorithms (SIM-RA) capable reconstructing super-resolution (SR) image with reduced operate in spatial domain. Here, we present frequency domain SIM-RA based on ordinary least squares technique, which enables SR using four Unlike RA, produces...
Advances in light-sheet microscopy have enabled the fast three-dimensional (3D) imaging of live cells and bulk specimens with low photodamage phototoxicity. Combining illumination super-resolution is expected to resolve subcellular structures. Actually, such kind was recently demonstrated using a single-molecule localization algorithm. However, depth temporal resolution this method are limited owing requirements precise single molecule reconstruction. In work, we present two-photon via...
Pre-chirp technique was used in an Nd-doped fiber amplifier to optimize high-quality 910 nm pulses with the width of 114 fs and pulse energy 4.4 nJ. The vivo zebrafish imaging results from our totally home-made microscopy proves femtosecond Nd laser ideal source two-photon microscopic imaging.
Total-internal-reflection fluorescence (TIRF) microscopy provides high optical-sectioning capability and a good signal-contrast ratio for structures near the surfaces of cells. In recent years, several improvements have been developed, such as variable-angle TIRF (VA-TIRF) spinning (sp-TIRF), which permit quantitative image analysis address non-uniform scattering fringes, respectively. Here, we present dual-color DMD-based shadowless-illuminated (siva-TIRF) system that uniform illumination...
We report a core-pumped all-normal dispersion mode-locked Nd-doped fiber laser at 910 and 935 nm. The pulse is compressed to 198 fs, the energy 1.3 nJ. slope efficiency more than 14%. This tested as optical source for two-photon fluorescence imaging of pollen.
Abstract It remains challenging to construct a complete cell lineage map of the origin vascular endothelial cells in any vertebrate embryo. Here, we report application toto light-sheet fluorescence imaging embryos trace (ECs) at single-cell resolution zebrafish. We first adapted previously reported method embryo mounting and imaging, created an alignment, fusion, extraction all-in-one software (AFEIO) for processing big data, performed quantitative analysis relationships using commercially...
How pancreatic β-cells acquire function in vivo is a long-standing mystery due to the lack of technology visualize β-cell living animals. Here, we applied high-resolution two-photon light-sheet microscope for first imaging Ca2+activity every Tg (ins:Rcamp1.07) zebrafish. We reveal that heterogeneity functional development occurred as two waves propagating from islet mantle core, coordinated by vascularization. Increasing amounts glucose induced acquisition and enhancement via activating...
Summary We developed a miniaturized two-photon microscope (MINI2P) for fast, high-resolution, multiplane calcium imaging of over 1,000 neurons at time in freely moving mice. With weight below 3g and highly flexible connection cable, MINI2P allowed to proceed with no impediment behavior half-hour free-foraging trials compared untethered, unimplanted animals. The improved cell yield was achieved through new optical system design featuring an enlarged field view (FOV) micro-tunable lens...
We demonstrate an 880 mW 780 nm femtosecond laser based on a high-order dispersion compensated Er-doped fiber and frequency doubling. It has been used two-photon three-axis digital scanned light-sheet microscopy.
Get PDF Email Share with Facebook Tweet This Post on reddit LinkedIn Add to CiteULike Mendeley BibSonomy Citation Copy Text X. Huang, Y. li, W. Zong, Zheng, Wu, S. Zhao, and l. Chen, "MEMS-Based Shadowless-illuminated variable-angle TIRF (SIVA-TIRF)," in CLEO: 2015, OSA Technical Digest (online) (Optica Publishing Group, 2015), paper ATh1J.3. Export BibTex Endnote (RIS) HTML Plain alert Save article
Summary The medial entorhinal cortex (MEC) creates a map of local space, based on the firing patterns grid, head direction (HD), border, and object-vector (OV) cells. How these cell types are organized anatomically is debated. In-depth analysis this question requires collection precise anatomical activity data across large populations neurons during unrestrained behavior, which neither electrophysiological nor previous imaging methods fully afford. Here we examined topographic arrangement...
Visualizing the function of pancreatic β-cells in vivo has been a long-sought goal for β-cell researchers. Unlike imaging mammalian species with conventional positron emission tomography and single-photon computed tomography, which only provides limited spatial-temporal resolution, transparent zebrafish embryos are unique model that allows high-resolution fluorescent their native physiological microenvironment vivo. Here, we detail protocol real-time visualization individual non-invasive...
Abstract A novel technique based on the remote-focusing concept, using a galvanometer scanner combined with self-fabricated “step mirror” or “tilted to transform fast lateral scanning into axial scanning, was reported as new solution for fast, subcellular, 3D fluorescence imaging.
We optimized pre-chirp in an Nd-doped fiber amplifier to achieve high-quality femtosecond 920 nm pulses. The vivo zebrafish imaging proves the laser ideal source two-photon microscopic imaging.
In this presentation we report a new 3D scanned DSLM. The system combined 1) two-photon excitation, 2) scanning along the illumination axis (x-axis) using tunable acoustic gradient lens (TAG) to stretch Rayleigh range [5], 3) vertically (y-axis) by one galvo mirror create light sheet. 4) Z-axis do fast imaging another mirror. image plane was kept aligned with z-axis sheet an electric (ETL) as described in ref. 6. can be tailored any shape between 50×50 μm2 and more than 500×500 constant...