A5091640228- Enzyme Structure and Function
- Ion Channels and Receptors
- Advanced Electron Microscopy Techniques and Applications
- Force Microscopy Techniques and Applications
- Ion channel regulation and function
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Advanced X-ray Imaging Techniques
- Bacteriophages and microbial interactions
- Genetics, Aging, and Longevity in Model Organisms
- Electron and X-Ray Spectroscopy Techniques
- Proteoglycans and glycosaminoglycans research
- Physiological and biochemical adaptations
- Mass Spectrometry Techniques and Applications
- Spaceflight effects on biology
- Medical Imaging Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- Nuclear Structure and Function
- Advanced MRI Techniques and Applications
- Magnetic and Electromagnetic Effects
- RNA Research and Splicing
- Phytochemicals and Antioxidant Activities
National Institute of Advanced Industrial Science and Technology
2016-2025
Yokohama City University
2019-2025
The University of Tokyo
2019-2023
City University of Seattle
2023
Ibaraki University
2022
Japan Synchrotron Radiation Research Institute
2021-2022
Daikin (Japan)
2021
Jichi Medical University
2021
Hokkaido University
2021
The Open University of Japan
2019
Canonical transient receptor potential (TRPC) channels control influxes of Ca(2+) and other cations that induce diverse cellular processes upon stimulation plasma membrane receptors coupled to phospholipase C (PLC). Invention subtype-specific inhibitors for TRPCs is crucial distinction respective TRPC play particular physiological roles in native systems. Here, we identify a pyrazole compound (Pyr3), which selectively inhibits TRPC3 channels. Structure-function relationship studies compounds...
Keap1 is a substrate adaptor of Cullin 3-based E3 ubiquitin ligase complex that recognizes Nrf2, and also acts as cellular sensor for xenobiotics oxidative stresses. Nrf2 transcriptional factor regulating the expression cytoprotective enzyme genes in response to such Under unstressed conditions binds results rapid degradation through proteasome pathway. In contrast, upon exposure electrophilic stress, reactive cysteine residues intervening region (IVR) Broad complex, Tramtrack, Bric-à-Brac...
CD44 is a cell surface adhesion molecule for hyaluronan and implicated in tumor invasion metastasis. Proteolytic cleavage of plays critical role the migration cells regulated by factors present microenvironment, such as oligosaccharides epidermal growth factor. However, molecular mechanisms underlying proteolytic on membranes remain poorly understood. In this study, we demonstrated that cholesterol depletion with methyl-β-cyclodextrin, which disintegrates membrane lipid rafts, enhances...
The Ca(2+) release-activated channel is a principal regulator of intracellular rise, which conducts various biological functions, including immune responses. This channel, involved in store-operated influx, believed to be composed at least two major components. Orai1 has putative pore and locates the plasma membrane, STIM1 sensor for luminal store depletion endoplasmic reticulum membrane. Here we have purified FLAG-fused protein, determined its tetrameric stoichiometry, reconstructed...
TRPA1 is a member of the transient receptor potential (TRP) cation channel family that expressed primarily on sensory neurons. This chemosensor activated through covalent modification multiple cysteine residues with wide range reactive compounds including allyl isothiocyanate (AITC), spicy component wasabi. The present study reports potent and selective agonists TRPA1, discovered screening 1657 electrophilic molecules. In an effort to validate mode action hit molecules, we noted new...
Measuring molecular dynamics improves understanding of the structure–function relationships materials. In this study, we present a novel technique for observing material using transmission electron microscopy (TEM), in which gold nanoparticles are employed as motion probes tracing polymer real space. A thin layer materials was generated on 2 μm diameter holes Quantifoil grids, and were dispersed membrane surface. By tracking movement from series TEM images taken under continuous temperature...
Abstract In this work we report findings on the study of 1,2,4,5-tetrabromobenzene (TBB) polymorphs by diffracted X-ray blinking (DXB). Using DXB it was found that ms-s time scale subtle fluctuations in d-spacing between lattice planes occurs more rapidly γ-phase polymorph than β-phase, successfully identifying another distinct characterization them. It also rate molecular motion spiked near phase transition temperature beyond typical characteristic regions. This offers additional insight...
Prestin is a transmembrane motor protein localized at the outer hair cells (OHCs) of mammalian inner ear. Voltage-dependent conformational changes in prestin generate length OHCs. A loss function reported to induce severe auditory deficiencies, suggesting prestin-dependent OHC may be least part cochlear amplification. Here we expressed recombinant FLAG-fused proteins Sf9 and purified particles uniform size EM. The square-shaped top view prestin, binding multiple anti-FLAG antibodies each...
A PCR method to detect porcine DNA was developed for verifying the allergen labeling of foods and identifying hidden pork ingredients in processed foods. The primer pair, F2/R1, designed gene encoding cytochrome b specific detection with high sensitivity. amplified fragment (130 bp) specifically detected from DNA, while no amplification occurred other species such as cattle, chicken, sheep, horse. When used investigating commercial food products, clearly those containing list ingredients. In...
Transient receptor potential melastatin type 2 (TRPM2) is a redox-sensitive, calcium-permeable cation channel activated by various signals, such as adenosine diphosphate ribose (ADPR) acting on the ADPR pyrophosphatase (ADPRase) domain, and cyclic ADPR. Here, we purified FLAG-tagged tetrameric TRPM2 channel, analyzed it using negatively stained electron microscopy, reconstructed three-dimensional structure at 2.8-nm resolution. This multimodal sensor molecule has bell-like shape of 18 nm in...
The membrane-anchored protease regulator RECK plays important roles in mammalian development and tumor suppression. biochemical bases of these bioactivities, however, remain poorly understood. Here we report on the properties a recombinant protein expressed mouse fibroblasts purified to near homogeneity. Multiple lines evidence indicate that forms dimers. Single particle reconstruction using transmission electron microscopy revealed unique cowbell-like shaped dimer. is cleaved by MMP-2 MMP-7...
Eukaryotic mature mRNAs possess a poly adenylate tail (poly(A)), to which multiple molecules of poly(A)-binding protein C1 (PABPC1) bind. PABPC1 regulates translation and mRNA metabolism by binding regulatory proteins. To understand functional mechanism the proteins, it is necessary reveal how exist on poly(A). Here, we characterize structure poly(A), using transmission electron microscopy (TEM), chemical cross-linking, NMR spectroscopy. The TEM images cross-linking results indicate that...