A5022916983- Ion channel regulation and function
- Nanopore and Nanochannel Transport Studies
- Lipid Membrane Structure and Behavior
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Pharmacological Effects of Natural Compounds
- Fuel Cells and Related Materials
- Phytochemistry and biological activity of medicinal plants
- Neuroscience and Neuropharmacology Research
- Chromatography in Natural Products
- Ion Channels and Receptors
- S100 Proteins and Annexins
- Analytical Chemistry and Chromatography
- Plant chemical constituents analysis
- Cell death mechanisms and regulation
- Medicinal Plants and Neuroprotection
- PARP inhibition in cancer therapy
- Plant Molecular Biology Research
- Electrochemical sensors and biosensors
- Trace Elements in Health
- Light effects on plants
- Metabolism and Genetic Disorders
- Polysaccharides and Plant Cell Walls
- Research in Cotton Cultivation
- Vibrio bacteria research studies
National University of Uzbekistan
2019-2025
National Institute for Physiological Sciences
2011-2021
Academy of Sciences Republic of Uzbekistan
1997-2019
Universidade Federal de Pernambuco
1997-2007
Staphylococcal alpha‐toxin forms homo‐oligomeric channels in lipid bilayers and cell membranes. Here, we report that electrophysiological monitoring of single‐channel function using a derivatized cysteine substitution mutant allows accurate determination the subunit stoichiometry oligomer situ. The phenotype formed planar with replacement I7C is equal to wild type. When pores were I7C, alterations several channel properties observed upon modification SH reagents. Decreases conductance then...
Introduction: Cell volume regulation is critical for cellular proliferation and death. Pinocembrin effectively suppresses the in thymocytes under hypoosmotic stress by blocking volume-sensitive anion channel. This study aims to evaluate effects of this flavonoid on thymocyte Methods: Thymocytes were cultured RPMI-1640 medium supplemented with 10% fetal bovine serum, cell number was determined cloud-based automated counting (Corning). Necrotic apoptotic death evaluated propidium iodide-...
BACKGROUND/AIMS Maxi-anion channel (Maxi-Cl) is ubiquitously expressed and involved in a number of important cell functions especially by serving as an ATP release pathway. We recently identified SLCO2A1 its essential core component. However, the regulatory component required for activation/inactivation remains unidentified. METHODS In present study, to identify component, we made genome-wide analysis combined with siRNA screening performed patch-clamp studies assay after gene silencing...
Abstract Gossypol is a natural polyphenol presently considered as promising biological phytochemical with range of activities including anticancer. We examined volume regulation‐dependent effects gossypol using erythrocytes and thymic lymphocytes. effectively lysed human red blood cells (RBC) half‐maximal concentration 67.4 ± 1.6 μmol/L in non‐colloid osmotic manner. Sublytic doses 1–10 significantly protected RBC from hemolysis, but potentiated their sensitivity to the colloid‐osmotic lysis...
Abstract The volume-sensitive outwardly rectifying anion channel (VSOR) is a key component of volume regulation system critical for cell survival in non-isosmotic conditions. aim the present study was to test effects four tannin extracts with defined compositions on and VSOR. Preparation I (98% hydrolysable tannins isolated from leaves sumac Rhus typhina L. ) II (100% broadleaf plantain Plantago major L completely irreversibly abolished swelling-activated VSOR currents HCT116 cells. Both...
The effect of pre-sowing seed treatment with immunostimulant Bisol-2, red light and low frequency electromagnetic field on the content fungitoxic substances phenolic nature – phytoalexins (isohemigossypol gossypol-equivalent) in infected etiolated cotton seedlings S-4727 cultivar Verticillium wilt pathogen was studied. It found that photostimulation seeds by induces phytoalexin formation tissues 1.5–2 times more effectively comparison Bisol-2 preparation or inducer nature. correlation...