A5050161301- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Agronomic Practices and Intercropping Systems
- Nematode management and characterization studies
- Plant responses to water stress
- Coastal wetland ecosystem dynamics
- Plant Parasitism and Resistance
- Soybean genetics and cultivation
- Cassava research and cyanide
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Ammonia Synthesis and Nitrogen Reduction
- Hemoglobin structure and function
- Antioxidant Activity and Oxidative Stress
- Microbial Community Ecology and Physiology
- Plant Pathogenic Bacteria Studies
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- Toxin Mechanisms and Immunotoxins
- Plant and Biological Electrophysiology Studies
- Medicinal Plants and Bioactive Compounds
- Studies on Chitinases and Chitosanases
- Phytase and its Applications
- Biochemical and Structural Characterization
- High Altitude and Hypoxia
Kagoshima University
2015-2024
Bioscience Research
2011
Graduate School USA
2010
University of Miyazaki
2009
Saga University
2009
University of California, Los Angeles
2009
GTx (United States)
2008
Institut des Sciences du Végétal
2005-2006
Institut de génétique et de développement de Rennes
2006
Centre National de la Recherche Scientifique
2005
The complete nucleotide sequence of the genome a symbiotic bacterium Bradyrhizobium japonicum USDA110 was determined. B. single circular chromosome 9,105,828 bp in length with an average GC content 64.1%. No plasmid detected. comprises 8317 potential protein-coding genes, one set rRNA genes and 50 tRNA genes. Fifty-two percent protein showed similarity to known function 30% hypothetical remaining 18% had no apparent reported Thirty-four significant those both Mesorhizobium loti Sinorhizobium...
Legume plants host nitrogen-fixing endosymbiotic Rhizobium bacteria in root nodules. In Medicago truncatula, the undergo an irreversible (terminal) differentiation mediated by hitherto unidentified plant factors. We demonstrated that these factors are nodule-specific cysteine-rich (NCR) peptides targeted to and enter bacterial membrane cytosol. Obstruction of NCR transport dnf1-1 signal peptidase mutant correlated with absence terminal differentiation. On contrary, ectopic expression NCRs...
Symbiosis between legumes and Rhizobium bacteria leads to the formation of root nodules where in infected plant cells are converted into nitrogen-fixing bacteroids. Nodules with a persistent meristem indeterminate, whereas without determinate. The symbiotic both nodule types polyploid because several cycles endoreduplication (genome replication mitosis cytokinesis) grow consequently extreme sizes. Here we demonstrate that differentiation bacteroids indeterminate Medicago related from...
Rhizobia are symbiotic nitrogen-fixing soil bacteria that associated with host legumes. The establishment of rhizobial symbiosis requires signal exchanges between partners in microaerobic environments result mutualism for the two partners. We developed a macroarray Mesorhizobium loti MAFF303099, microsymbiont model legume Lotus japonicus, and monitored transcriptional dynamics bacterium during symbiosis, microaerobiosis, starvation. Global profiling demonstrated clusters genes within island...
The effects of the phytohormone abscisic acid (ABA) on plant growth and root nodule formation were analyzed in Trifolium repense (white clover) Lotus japonicus, which form indeterminate determinate nodules, respectively. In T. repense, although number nodules formed after inoculation with Rhizobium leguminosarum bv. trifolii strain 4S (wild type) was slightly affected by exogenous ABA, those H1(pC4S8), forms ineffective dramatically reduced 28 days (DAI). At 14 21 DAI, wild-type decreased...
We characterized the expression profiles of LjHb1 and LjHb2, non-symbiotic hemoglobin (non-sym-Hb) genes Lotus japonicus. Although LjHb2 showed 77% homology in their cDNA sequences, is located a unique position phylogenetic tree plant Hbs. The 5′-upstream regions both contain motif AAAGGG at similar to that promoters other non-sym-Hb genes. Expression obtained by using quantitative RT–PCR were expressed all tissues mature plants, was enhanced root nodules. strongly induced under hypoxic cold...
Nitric oxide (NO) has gained interest as a major signaling molecule during plant development and in response to environmental cues. Formation of NO symbiotic interactions been reported, but the role sources nodules remain unclear. In this work, involvement denitrification, performed by symbiont Bradyrhizobium japonicum, formation soybean flooding conditions investigated inoculating plants with napA-, nirK-, or norC-deficient mutants. Levels nitrosylleghemoglobin (LbNO) flooded nirK norC were...
Light is critical for supplying carbon to the energetically expensive, nitrogen-fixing symbiosis between legumes and rhizobia. Here, we show that phytochrome B (phyB) part of monitoring system detect suboptimal light conditions, which normally suppress Lotus japonicus nodule development after Mesorhizobium loti inoculation. We found number nodules produced by L. phyB mutants significantly reduced compared with WT Miyakojima MG20. To explore causes other than photoassimilate production,...
Symbiotic nitrogen fixation by the collaboration between leguminous plants and rhizobia is an important system in global cycle, some molecular aspects during early stage of host-symbiont recognition have been revealed. To understand responses a host plant against various bacteria, we examined expression hemoglobin (Hb) genes production nitric oxide (NO) Lotus japonicus after inoculation with or pathogens. When symbiotic rhizobium Mesorhizobium loti was inoculated, LjHb1 NO were induced...
Plant hemoglobins (Hbs) have been divided into three groups: class 1, 2, and truncated Hbs. The various physiological functions of 1 Hb include its role as a modulator nitric oxide (NO) levels in plants. To gain more insight the Hbs, we investigated physical properties LjHb1 AfHb1, Hbs model legume Lotus japonicus an actinorhizal plant Alnus firma, respectively. Spectrophotometric analysis showed that recombinant form AfHb1 proteins reacted with NO. localization expression was correlated...
Ten strains of root nodule bacteria were isolated from the nodules Acacia mangium grown in Philippines and Thailand. Partial sequences (approx. 300 bp) 16S rRNA gene each isolate analyzed. The nucleotide strain DASA 35030 indicated high homology (>99%) with members genus Ochrobactrum Brucellaceae, although other isolates homologous to those two distinct genera Bradyrhizobium Rhizobium. was strongly suggested be a by full length gene, fatty acids composition, G+C contents DNA, physiological...
Actinorhizal symbiosis is as important in biological nitrogen fixation legume-rhizobium the global cycle. To understand function of hemoglobin (Hb) actinorhizal symbiosis, we characterized a Hb Alnus firma, AfHb1. A cDNA that encodes nonsymbiotic (nonsym-Hb) was isolated from library A. firma nodules probed with LjHb1, nonsym-Hb Lotus japonicus. No homolog symbiotic (sym-Hb) could be identified by screening or polymerase chain reaction (PCR) using degenerate primers for other sym-Hb genes....
The phytohormone abscisic acid (ABA) is known to be a negative regulator of legume root nodule formation. By screening Lotus japonicus seedlings for survival on an agar medium containing 70 microM ABA, we obtained mutants that not only showed increased number but also enhanced nitrogen fixation. mutant was designated fixation1 (enf1) and confirmed monogenic incompletely dominant. low sensitivity ABA phenotype thought result from either decrease in the concentration plant's endogenous or...
Bradyrhizobium sp. DOA9 isolated from the legume Aeschynomene americana exhibited a broad host range and divergent nodulation (nod) genes compared with other members of Bradyrhizobiaceae. Genome analysis revealed that its genome comprised single chromosome 7.1 Mbp plasmid 0.7 Mbp. The showed highest similarity nod gene-harboring soybean symbiont B. japonicum USDA110, whereas pBBta01 gene-lacking photosynthetic strain BTAi1, which nodulates species. Unlike in bradyrhizobia, encodes related to...
TrEnodDR1 (Trifolium repens early nodulin downregulation 1) encodes a coat protein of White clover cryptic virus 1. Its expression in white was down-regulated at the time when root nodules formed. We surmised that its artificial would interfere with nodulation. Therefore, we investigated effects on growth and nodulation Lotus japonicus (a model legume). Transformants were prepared by Agrobacterium spp.-mediated transformation. The transformants reduced number per unit length greatly...
To better understand the molecular responses of plants to arbuscular mycorrhizal (AM) fungi, we analyzed differential gene expression patterns Lotus japonicus , a model legume, with aid large-scale cDNA macroarray. Experiments were carried out considering effects contaminating microorganisms in soil inoculants. When colonization by AM i.e. Glomus mosseae and Gigaspora margarita was well established, four cysteine protease genes induced. In situ hybridization revealed that these specifically...
The diversity of bacteria nodulating Aeschynomene americana L. in Thailand was determined from phenotypic characteristics and multilocus sequence analysis the 16S rRNA gene 3 housekeeping genes (dnaK, recA, glnB). isolated strains were nonphotosynthetic assigned to genus Bradyrhizobium, which B. yuanmingense dominant species. Some other species, including japonicum, liaoningense, canariense, minor These divided into 2 groups-nod-containing divergent nod-containing strains-based on Southern...
Leghemoglobins transport and deliver O2 to the symbiosomes inside legume nodules are essential for nitrogen fixation. However, roles of other hemoglobins (Hbs) in rhizobia–legume symbiosis unclear. Several Lotus japonicus mutants affecting LjGlb1-1, a non-symbiotic class 1 Hb, have been used study function this protein symbiosis. Two TILLING alleles with single amino acid substitutions (A102V E127K) LORE1 null allele retrotransposon insertion 5′-untranslated region (96642) were selected...
The class 1 phytoglobin, LjGlb1-1, is expressed in various tissues of the model legume Lotus japonicus, where it may play multiple functions by interacting with nitric oxide (NO). One such onset a proper symbiosis Mesorhizobium loti resulting formation actively N2-fixing nodules. Stable overexpression lines (Ox1 and Ox2) LjGlb1-1 were generated phenotyped. Both Ox showed reduced NO levels roots enhanced nitrogenase activity mature senescent nodules relative to wild-type (WT). Physiological...
Bradyrhizobium sp. strain SUTN9-2 was confirmed as rice endophytic bacteria and also growth promotion agent. showed the capability of plant characteristics, such indole-3-acetic acid (IAA) 1-amino-cyclopropane-1-carboxylic (ACC) deaminase productions nitrogen fixation. In this study, ability to stimulate investigated at different stages with N-free NH4 NO3 under in vivo condition. The dry weight chlorophyll content could be enhanced when inoculated N-free, especially seedling stage (7 14...
Aphids have a mutualistic relationship with the bacterial endosymbiont Buchnera aphidicola. We previously reported seven cysteine-rich peptides in pea aphid Acyrthosiphon pisum and named them Bacteriocyte-specific Cysteine-Rich (BCR) peptides; these are exclusively expressed bacteriocytes, special cells that harbor symbionts. Similar symbiotic organ-specific identified root nodules of leguminous plants Nodule-specific (NCR) peptides. NCR target rhizobia essential for nitrogen fixation. A...