A5081410394- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- Wastewater Treatment and Nitrogen Removal
- Agronomic Practices and Intercropping Systems
- Soybean genetics and cultivation
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Microbial Community Ecology and Physiology
- Plant-Microbe Interactions and Immunity
- Cassava research and cyanide
- Bacteriophages and microbial interactions
- Polyamine Metabolism and Applications
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Microbial Fuel Cells and Bioremediation
- Growth and nutrition in plants
- Phytase and its Applications
- Genomic variations and chromosomal abnormalities
- Prenatal Screening and Diagnostics
- Plant Micronutrient Interactions and Effects
- Coastal wetland ecosystem dynamics
- Plant pathogens and resistance mechanisms
- Infections and bacterial resistance
Universidad Nacional Autónoma de México
2014-2025
National Institute of Genomic Medicine
2023
Universidad Autónoma del Estado de Morelos
2013-2016
University of California, Santa Cruz
2015
Center for Genomic Science
2007-2008
The genus Burkholderia includes pathogens of plants and animals some human opportunistic pathogens, such as the cepacia complex (Bcc), but most species are nonpathogenic, plant associated, rhizospheric or endophytic. Since rhizobacteria expressing ACC (1-aminocyclopropane-1-carboxylate) deaminase may enhance growth by lowering ethylene levels, in this work we investigated presence activity acdS gene 45 strains, which representing 20 well-known species. results demonstrated that is a...
ABSTRACT Phosphorus (P), an essential element for plants, is one of the most limiting nutrients plant growth. A few transcription factor (TF) genes involved in P‐starvation signalling have been characterized Arabidopsis thaliana and rice. Crop production common bean ( Phaseolus vulgaris L.), important legume human consumption, often limited by low P soil. Despite its agronomic importance, nothing known about transcriptional regulation P‐deficient plants. We functionally P‐deficiency‐induced...
Micro-RNAs are recognized as important posttranscriptional regulators in plants. The relevance of micro-RNAs the legume-rhizobia nitrogen-fixing symbiosis is emerging. objective this work was to functionally characterize role micro-RNA172 (miR172) and its conserved target APETALA2 (AP2) transcription factor common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis. Our expression analysis revealed that mature miR172c increased upon rhizobial infection continued increasing during nodule...
Nitric oxide (NO) has gained interest as a major signaling molecule during plant development and in response to environmental cues. Formation of NO symbiotic interactions been reported, but the role sources nodules remain unclear. In this work, involvement denitrification, performed by symbiont Bradyrhizobium japonicum, formation soybean flooding conditions investigated inoculating plants with napA-, nirK-, or norC-deficient mutants. Levels nitrosylleghemoglobin (LbNO) flooded nirK norC were...
Several lines of evidence suggest that reiterated sequences in the human genome are targets for nonallelic homologous recombination (NAHR), which facilitates genomic rearrangements. We have used a PCR-based approach to identify breakpoint regions rearranged structures genome. In particular, we identified intrachromosomal identical repeats located reverse orientation, may lead chromosomal inversions. A bioinformatic workflow pathway select appropriate analysis was developed. Three such...
Phylogenetic analysis of 16S rRNA, nodC, and nifH genes four bacterial strains isolated from root nodules Phaseolus vulgaris grown in Morocco soils were identified as Burkholderia phymatum. All formed N(2)-fixing on P. Mimosa, Acacia, Prosopis species reduced acetylene to ethylene when cultured ex planta.
The canonical two-component systems (TCS) consist of a histidine kinase and response regulator that work together to control various pathways in bacteria. Rhizobia are rod-shaped, Gram-negative alpha-proteobacteria capable establishing nitrogen-fixing symbiosis with compatible legume hosts. These bacteria can live freely the soil or as intracellular symbionts within root nodules. Here, we characterized an orphan OmpR-type Rhizobium etli CE3, which renamed CenR due its similarity proteins...
ABSTRACT Rhizobial bacteria are commonly found in soil but also establish symbiotic relationships with legumes, inhabiting the root nodules, where they fix nitrogen. Endophytic rhizobia have been reported roots and stems of legumes other plants. We isolated several rhizobial strains from nodules noninoculated bean plants looked for their provenance interiors seeds. Nine isolates were obtained, covering most known symbiont species, which belong to Rhizobium Sinorhizobium groups. The showed...
Among the complexities in regulation of nitrogen fixation Rhizobiaceae are reiteration regulatory components as well variant roles for each component between species. For Rhizobium etli CFN42, we reported that symbiotic plasmid (pCFN42d) contains a key gene (fixKd) and genes cytochrome oxidase (fixNOQPd). Here discuss occurrence these (fixKf fixNOQPf) finding an unusual fixL homolog on previously considered cryptic (pCFN42f). The structure deduced FixL polypeptide is suggestive fusion...
Abstract Background TIFY is a large plant-specific transcription factor gene family. A subgroup of genes named JAZ (Jasmonate-ZIM domain) has been identified as repressors jasmonate (JA)-regulated in Arabidopsis and other plants. JA signaling involved many aspects plant growth/development defense responses to biotic abiotic stresses. Here, we the (designated PvTIFY ) from legume common bean ( Phaseolus vulgaris) functionally characterized PvTIFY10C transcriptional regulator. Results Nineteen...
Abstract Background Bean-nodulating Rhizobium etli originated in Mesoamerica, while soybean-nodulating Sinorhizobium fredii evolved East Asia. S. strains, such as GR64, have been isolated from bean nodules Spain, suggesting the occurrence of conjugative transfer events between introduced and native strains. In R. CFN42, symbiotic plasmid (pRet42d) requires cointegration with endogenous self-transmissible pRet42a. Aiming at further understanding generation diversity among nodulating we...
To investigate the involvement of Rhizobium etli cbb(3) oxidase in response Phaseolus vulgaris to drought, common bean plants were inoculated with R. strain, CFNX713, overexpressing this bacteroids (cbb(3)(+)) and subjected drought conditions. The negative effect on plant nodule dryweight, nitrogen content, functionality was more pronounced wild-type (WT) strain than those cbb(3)(+) strain. Regardless treatment, produced by showed higher respiratory capacity WT Inoculation alleviated a...
Crop production of the important legume, common bean (Phaseolus vulgaris), is often limited by low phosphorus (P) in soil. The genotypes, BAT477 and DOR364, have contrasting responses to P starvation. Plants from deficiency tolerant genotype showed higher phosphate content root biomass as compared DOR364 plants under PvPHR1 transcription factor-signaling pathway plays an essential role response PvPHO2, a negative regulator this pathway, encodes ubiquitin E2 conjugase that promotes...
Frequent tandem amplification of defined regions the genome, called amplicons, is a common characteristic in genomes some Rhizobium species, such as etli. In order to map these zones model replicon, we undertook an analysis plasticity patterns fostered by amplicons pSym (390 kb) R. etli CFN42. Data presented this article indicate presence four pSym, used for generation amplifications and deletions. The are large, ranging from 90 175 kb, they overlapping. Each amplicon usually flanked...
ABSTRACT Previously, we reported finding duplicated fixNOQP operons in Rhizobium etli CFN42. One of these is located the symbiotic plasmid ( d), while other a cryptic f). Although novel FixL-FixKf regulatory cascade participates microaerobic expression both operons, found that mutation fixL eliminates f but has only moderate effect on d. This suggests there are differential controls. Interestingly, d operon was essential for nitrogen fixation (L. Girard, S. Brom, A. Dávalos, O. Lopez, M....
ABSTRACT Plasmid p42a from Rhizobium etli CFN42 is self-transmissible and indispensable for conjugative transfer of the symbiotic plasmid (pSym). Most pSym transconjugants also inherit p42a. that lack always contain recombinant pSyms, which we designated RpSyms*. RpSyms* do not some segments instead have sequences, including replication regions. These novel plasmids are compatible with wild-type pSym, incompatible p42a, self-transmissible. The features derivatives simultaneously containing a...
Phosphate (Pi) deficiency reduces nodule formation and development in different legume species including common bean. Despite significant progress the understanding of genetic responses underlying adaptation nodules to Pi deficiency, it is still unclear whether this nutritional interferes with molecular dialogue between legumes rhizobia. If so, what part impaired? In study, we provide evidence demonstrating that negatively affects critical early physiological are required for a successful...
Rhizobia are soil bacteria that establish symbiotic relationships with legumes and fix nitrogen in root nodules. We recently reported several nitrogen-fixing rhizobial strains, belonging to Rhizobium phaseoli, R. trifolii, grahamii Sinorhizobium americanum, were able colonize Phaseolus vulgaris (common bean) seeds. To gain further insight into the traits support this ability, we analyzed genomic sequences proteomes of phaseoli (CCGM1) S. americanum (CCGM7) strains from seeds compared them...
Summary: The replicator region of the symbiotic plasmid Rhizobium etli CFN42 was cloned and sequenced. A derivative (pH3) harbouring a 5.6 kb Hin dIII fragment from found to be capable independent replication eliminated when introduced into R. CFNX101 strain ( recA derivative). stability copy number pH3 were same as that plasmid, indicating information required for stable incompatibility resides in fragment. sequence analysis this showed presence three ORFs similar organization repA, repB...
In Rhizobium etli the nitrogenase reductase genes are reiterated. Strain CE3 has three copies; nifHa and nifHb form part of nifHDK operons with structural genes, while nifHc is linked to a truncated nifD homolog. Their sequences identical up 6 residues upstream from sigma54-dependent promoter. A remarkable difference among them absence canonical NifA binding sites site located 200 bp nifHb. To evaluate transcriptional regulation reiterated nifH we constructed fusions lacZ gene Escherichia...
An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, Olivares, and Sanjuán, Bacteriol. 180:4583-4590, 1998) was modified improve its reliability prevent selection undesired false mob clones. By following this approach, we were able two regions the Rhizobium etli CFN42. One corresponds recently characterized region nonsymbiotic, self-transmissible plasmid pRetCFN42a (C....
Rhizobium etli CFN42 is unable to use nitrate for respiration and lacks reductase activity as well the nap or nar genes encoding respiratory reductase. However, proteins closely related denitrification enzymes, norCBQD gene cluster a novel nirKnirVnnrRnnrU operon are located on pCFN42f. In this study, we carried out genetic functional characterization of reductases encoded by R. nirK norCB genes. By fusion expression analysis in free-living conditions, determined that regulates its response...
More than two-thirds of the powerful greenhouse gas nitrous oxide (N2O) emissions from soils can be attributed to microbial denitrification and nitrification processes. Bacterial reactions are catalyzed by periplasmic (Nap) or membrane-bound (Nar) nitrate reductases, nitrite reductases (NirK/cd1Nir), nitric (cNor, qNor/ CuANor), reductase (Nos) encoded nap/nar, nir, nor nos genes, respectively. Rhizobium etli CFN42, microsymbiont common bean, is unable respire under anoxic conditions perform...