A5064429412- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Bacterial Genetics and Biotechnology
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- Plant Micronutrient Interactions and Effects
- Coastal wetland ecosystem dynamics
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Wastewater Treatment and Nitrogen Removal
- RNA and protein synthesis mechanisms
- Cassava research and cyanide
- Plant-Microbe Interactions and Immunity
- Photosynthetic Processes and Mechanisms
- Microbial Fuel Cells and Bioremediation
- Finance, Taxation, and Governance
- Digital Transformation in Industry
- CRISPR and Genetic Engineering
- Enterobacteriaceae and Cronobacter Research
- Protein Structure and Dynamics
- Genomics and Chromatin Dynamics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Aquaculture disease management and microbiota
- Biocrusts and Microbial Ecology
Universidad Nacional Autónoma de México
2012-2024
Universidad de Huelva
2023
Meso Scale Discovery (United States)
2021
Tecnológico de Monterrey
2018-2020
Chalmers University of Technology
2019
Instituto de Medicina Genómica
2014
Center for Genomic Science
2014
In-Q-Tel
2014
Marquette University
2008
GTx (United States)
1999-2000
Rhizobium leguminosarum bv. phaseoli CFN42 contains six plasmids (pa to pf), and pd has been shown be the symbiotic plasmid. To determine participation of other in cellular functions, we used a positive selection scheme isolate derivatives cured each These were obtained for all except one (pe), which only deleted recovered. In regard symbiosis, found that addition pd, pb is also indispensable nodulation, partly owing presence genes involved lipopolysaccharide synthesis. The contribution pb,...
Experience from different laboratories indicates that Rhizobium strains can generate variability in regard to some phenotypic characteristics such as colony morphology or symbiotic properties. On the other hand, several reports suggest under certain stress conditions genetic manipulations cells present genomic rearrangements. In search of frequent rearrangements, we analyzed three laboratory are not considered cause bacterial populations. DNAs direct descendants a single cell were...
Repeated DNA sequences are a general characteristic of eucaryotic genomes. Although several examples reiteration have been found in procaryotic organisms, only the case archaebacteria Halobacterium halobium and volcanii [C. Sapienza W. F. Doolittle, Nature (London) 295:384-389, 1982], has reported as common genomic feature. The genomes two Rhizobium phaseoli strains, one meliloti strain, Agrobacterium tumefaciens strain were analyzed for presence repetitive DNA. spp. closely related soil...
ABSTRACT Replicon architecture in bacteria is commonly comprised of one indispensable chromosome and several dispensable plasmids. This view has been enriched by the discovery additional chromosomes, identified mainly localization rRNA and/or tRNA genes, also experimental demonstration their requirement for cell growth. The genome Rhizobium etli CFN42 constituted six large plasmids, ranging size from 184 to 642 kb. Five plasmids are viability, but plasmid p42e unusually stable. One...
One remarkable characteristic of the genomes some Rhizobium species is frequent occurrence rearrangements. In instances these rearrangements alter symbiotic properties strains. However, no detailed molecular mechanisms have been proposed for generation To understand involved in formation genome phaseoli, we designed a system which allows positive selection amplification and deletion events. We applied this to investigate stability plasmid R. phaseoli. High-frequency events were detected...
Gene conversion is defined as the non-reciprocal transfer of information between homologous sequences.Despite methodological problems to establish non-reciprocity, gene has been demonstrated in a wide variety bacteria.Besides examples high-frequency reversion mutations repeated genes, bacterial genomes implicated concerted evolution multigene families.Gene also prime importance generation antigenic variation, an interesting mechanism whereby some pathogens are able avoid host immune...
This work centres on the genomic comparisons of two closely-related nitrogen-fixing symbiotic bacteria, Rhizobium leguminosarum biovar viciae 3841 and etli CFN42. These strains maintain a stable core that is also common to other rhizobia species plus very variable significant accessory component. The chromosomes are highly syntenic, whereas plasmids related by fewer syntenic blocks have mosaic structures. pairs p42f-pRL12, p42e-pRL11 p42b-pRL9 as well large parts p42c with pRL10 shown be...
Based on the DNA sequence of symbiotic plasmid Rhizobium strain NGR234, we predicted potential rearrangements generated by homologous recombination. All were identified experimentally using a PCR-based methodology. Thus, and actual dynamic maps replicon coincide. By an approach that does not involve introduction exogenous genetic elements, derivative populations are pure for specific obtained. We propose knowledge genome offers possibility designing pathways sequential leading to alternative...
Among the complexities in regulation of nitrogen fixation Rhizobiaceae are reiteration regulatory components as well variant roles for each component between species. For Rhizobium etli CFN42, we reported that symbiotic plasmid (pCFN42d) contains a key gene (fixKd) and genes cytochrome oxidase (fixNOQPd). Here discuss occurrence these (fixKf fixNOQPf) finding an unusual fixL homolog on previously considered cryptic (pCFN42f). The structure deduced FixL polypeptide is suggestive fusion...
ABSTRACT An analysis of the conjugative transfer pRetCFN42d, symbiotic plasmid (pSym) Rhizobium etli , has revealed a novel gene, rctA as an essential element regulatory system for silencing R. pSym by repressing transcription conjugal genes in standard laboratory media. The gene product lacks sequence conservation with other proteins known function but may belong to winged-helix DNA-binding subfamily transcriptional regulators. Similar that many repressors, seems be positively...
The complete physical map of the symbiotic plasmid Rhizobium leguminosarum bv. phaseoli strain CFN42 was established. data support concept that genes are part a complex genomic structure which contains large amount reiterated DNA sequences. This is circular 390 kb with approximately 10 families internally sequences two to three elements each. One family includes directly oriented nitrogenase operons situated 120 apart. We also found several stretches pSym in other replicons cell....
Sustainable production of lignocellulosic biomass for energy use can contribute to climate change mitigation. This work aims compare the twelve fast-growing woody taxa belonging Eucalyptus, Casuarina, Populus and Paulownia genera, quality their its valorizing through transformation into pellets, as well effect crop on soil. Over course two rotations, plant growth were assessed. The yield aboveground dry ranged from 9 61 Mg ha−1 year−1 (equivalent 137–867 GJ year−1). highest yields obtained...
High-frequency genomic rearrangements affecting the plasmids of Rhizobium leguminosarum bv. phaseoli CFN42 were analyzed. This strain contains six large ranging in size from 200 to 600 kb. In absence any selective pressure, we found 11 strains 320 analyzed colonies that presented different kinds plasmid-borne rearrangements, including sequence amplification, deletion, cointegration, and loss plasmids. These data support concept R. genome is a dynamic structure imply are mixtures similar but...
Tyrosinase (EC 1.14.18.1) is a monophenol oxidase responsible for the synthesis of black pigment known as melanin. The tyrosinase gene <i>(melA)</i> plasmid-encoded in many rhizobial species. In <i>Rhizobium etli</i> CFN42, genetic location <i>melA</i> symbiotic plasmid (p42d) and its RpoN-NifA regulation suggest an involvement symbiosis. this work, we analyzed phenotype streptomycin-resistant derivative CFN42 (CE3), mutant (SP2) complemented strain...
Abstract Background Bean-nodulating Rhizobium etli originated in Mesoamerica, while soybean-nodulating Sinorhizobium fredii evolved East Asia. S. strains, such as GR64, have been isolated from bean nodules Spain, suggesting the occurrence of conjugative transfer events between introduced and native strains. In R. CFN42, symbiotic plasmid (pRet42d) requires cointegration with endogenous self-transmissible pRet42a. Aiming at further understanding generation diversity among nodulating we...
The bacterial genus Rhizobium comprises diverse symbiotic nitrogen-fixing species associated with the roots of plants in Leguminosae family. Multiple genomic clusters defined by whole genome comparisons occur within Rhizobium, but their equivalence to is controversial. In this study we investigated such ascertain significance a phylogeny context. Phylogenomic inferences based on complete sets ribosomal proteins and stringent core markers revealed main lineages Rhizobium. clades corresponding...
Frequent tandem amplification of defined regions the genome, called amplicons, is a common characteristic in genomes some Rhizobium species, such as etli. In order to map these zones model replicon, we undertook an analysis plasticity patterns fostered by amplicons pSym (390 kb) R. etli CFN42. Data presented this article indicate presence four pSym, used for generation amplifications and deletions. The are large, ranging from 90 175 kb, they overlapping. Each amplicon usually flanked...