- Legume Nitrogen Fixing Symbiosis
- Respiratory viral infections research
- Nematode management and characterization studies
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Child Nutrition and Water Access
- Antifungal resistance and susceptibility
- Plant nutrient uptake and metabolism
- Microbial Metabolism and Applications
- Plant Pathogenic Bacteria Studies
- SARS-CoV-2 and COVID-19 Research
- Plant Virus Research Studies
- Coastal wetland ecosystem dynamics
- Cassava research and cyanide
- Plant-Microbe Interactions and Immunity
- Biochemical and Structural Characterization
- Antibiotic Resistance in Bacteria
- Evolution and Genetic Dynamics
- Microbial Metabolic Engineering and Bioproduction
- Agronomic Practices and Intercropping Systems
- Potato Plant Research
- Animal Virus Infections Studies
Universidad Nacional Autónoma de México
2012-2025
Instituto de Salud Pública de Chile
2011-2023
Universidad Autónoma del Estado de Morelos
2002-2023
Ministry of Health
2022
National Institute of Genomic Medicine
2021
Instituto Politécnico Nacional
2021
Weatherford College
2021
Uman National University of Horticulture
2021
University of Groningen
2020
Ministry of Health
2020
We report the complete 6,530,228-bp genome sequence of symbiotic nitrogen fixing bacterium Rhizobium etli . Six large plasmids comprise one-third total size. The chromosome encodes most functions necessary for cell growth, whereas few essential genes or metabolic pathways are located in plasmids. Chromosomal synteny is disrupted by related to insertion sequences, phages, plasmids, and cell-surface components. Plasmids do not show synteny, their orthologs mostly shared accessory replicons...
We describe the epidemiological characteristics, pattern of circulation, and geographical distribution influenza B viruses its lineages using data from Global Influenza Study. included over 1.8 million cases occurred in thirty-one countries during 2000–2018. calculated proportion caused by lineages; determined timing A epidemics; compared age B/Victoria B/Yamagata cases; evaluated frequency lineage-level mismatch for trivalent vaccine. The median virus was 23.4%, with a tendency (borderline...
Respiratory syncytial virus (RSV) infections are one of the leading causes lower respiratory tract and have a major burden on society. For prevention control to be deployed effectively, an improved understanding seasonality RSV is necessary.The main objective this study was contribute better by examining GERi multi-country surveillance dataset.RSV seasons were included in analysis if they contained ≥100 cases. Seasonality determined using "average annual percentage" method. Analyses...
Abstract Background Respiratory syncytial virus (RSV) is one of the leading causes acute respiratory tract infections. To optimize control strategies, a better understanding global epidemiology RSV critical. this end, we initiated Global Epidemiology in Hospitalized and Community care study (GERi). Methods Focal points from 44 countries were approached to join GERi share detailed surveillance data. Countries completed questionnaire on characteristics their system. Results Fifteen provided...
Highlights Here we present the first human case of Influenza A H5N1 infection in Chile, and fifth worldwide 2023.The patient is a 53-year-old man who lives north region near seashore.The Chilean sample was subtyped clade 2.3.4.4b.
Several lines of evidence suggest that reiterated sequences in the human genome are targets for nonallelic homologous recombination (NAHR), which facilitates genomic rearrangements. We have used a PCR-based approach to identify breakpoint regions rearranged structures genome. In particular, we identified intrachromosomal identical repeats located reverse orientation, may lead chromosomal inversions. A bioinformatic workflow pathway select appropriate analysis was developed. Three such...
The current pandemic caused by the new coronavirus is a worldwide public health concern. To aboard this emergency, and like never before, scientific groups around world have been working in fast coordinated way to get maximum of information about virus when it has almost 3 months since first cases were detected Wuhan province China. complete genome sequences 450 isolates are available, studies similarities differences among them with close related viruses that similar epidemics century. In...
Bacillus velezensis 83 was isolated from mango tree phyllosphere of orchards located in El Rosario, Sinaloa, México. The assessment this strain as BCA (biological control agent), well PGPB (plant growth-promoting bacteria), were demonstrated through vivo and vitro assays. In assays showed that B. able to anthracnose (Kent mangoes) efficiently chemical treatment with Captan 50 PH™ or Cupravit hidro™. inoculation the roots maize seedlings yielded an increase 12% height 45% root biomass,...
Fabaceae (legumes) is one of the largest families flowering plants, and some members are important crops. In contrast to what we know about their great diversity or economic importance, our knowledge at genomic level chloroplast genomes (cpDNAs plastomes) for these crops limited. We sequenced complete genome common bean (Phaseolus vulgari s cv. Negro Jamapa) chloroplast. The plastome P. vulgaris a 150,285 bp circular molecule. It has gene content similar that other legume plastomes, but...
ABSTRACT In this work, we isolated and characterized 14 bacteriophages that infect Rhizobium etli . They were obtained from rhizosphere soil of bean plants agricultural lands in Mexico using an enrichment method. The host range these phages was narrow but variable within a collection 48 R. strains. We the complete genome sequence nine phages. Four resistant to several restriction enzymes vivo cloning, probably due nucleotide modifications. size sequenced varied 43 kb 115 kb, with median ∼45...
Although several studies have reported attenuated influenza illness following vaccination, results been inconsistent and focused predominantly on adults in the USA. This study aimed to evaluate severity of by vaccination status a broad range vaccine target groups across multiple South American countries.We analysed data from four countries (Argentina, Brazil, Chile, Paraguay) participating REVELAC-i, multicentre, test-negative design, effectiveness network including 41 sentinel hospitals....
This study aimed to investigate the impact of various common beans ( Phaseolus vulgaris L.) cultivars on bacterial communities in rhizosphere under local agricultural conditions. Even though differences cultivation history and physicochemical properties nearby agriculture plots, community bulk soil was quite similar more diverse than that rhizosphere. The closely between Black Bayo bean but differs from Pinto Saltillo collected a different season. A shared group within across specific taxa...
Rhizobium etli CFN42 contains six plasmids. Only one of them, p42a, is self-conjugative at high frequency. This plasmid strictly required for mobilization the symbiotic (pSym). To study transfer mechanism a self-transmissible cosmid clone containing its region was isolated. Its sequence showed that most tra genes are highly similar to Agrobacterium tumefaciens pTiC58 and other related Four putative regulatory were identified; three these (traI, traR, cinR) belong LuxR-LuxI family....
ABSTRACT Strains of the same bacterial species often show considerable genomic variation. To examine extent such variation in Rhizobium etli , complete genome sequence R. CIAT652 and partial sequences six additional strains having different geographical origins were determined. The compared with each other previously reported CFN42. DNA common to all constituted greater part these genomes localized both chromosome large plasmids. About 700 1,000 kb that did not match CFN42 was unique strain....
Although bacteria of the genus Wolbachia induced significant extended phenotypes to infected hosts, most molecular mechanisms involved are still unknown. To gain insight into bacterial genetic determinants, we sequenced whole genome wAlbB strain, a commensal obligate intracellular tiger mosquito Aedes albopictus.
Bacteriophages play significant roles in the composition, diversity, and evolution of bacterial communities. Despite their importance, it remains unclear how phage diversity phage-host interactions are spatially structured. Local adaptation may a key role. Nitrogen-fixing symbiotic bacteria, known as rhizobia, have been shown to locally adapt domesticated common bean at its Mesoamerican Andean sites origin. This affect phage-rhizobium interactions. However, knowledge about coevolution phages...
Introduction The increased availability of influenza surveillance data in recent years justifies an actual and more complete overview epidemiology Latin America. We compared the systems assessed A B, including spatio-temporal patterns epidemics, ten countries sub-national regions Methods aggregated by year country characteristics eighty-two were analysed. calculated median proportion laboratory-confirmed cases caused each virus strain, timing amplitude primary secondary peaks between...
The pandemic caused by the new coronavirus SARS-CoV-2 is a worldwide public health concern. First confined in China, and then disseminated widely across Europe America, has impacted moved scientific community around world to working fast coordinated way collect all possible information about this virus generate strategies protocols try stop infection. During March 2020, more than 16,000 full viral genomes have been shared databases, that allow construction of genetic landscapes for tracking...
Vaccination generates a neutralizing immune response against SARS-CoV-2. The genomic surveillance is showing the emergence of variants with mutations in spike, main target antibodies. To understand impact these variants, we report neutralization potency alpha, gamma, and D614G SARS-CoV-2 44 individuals that received two doses CoronaVac vaccine, an inactivated vaccine. Plasma samples collected at 60 days after second dose were analyzed by reduction cytopathic effect Vero E6 cells three...
Abstract Background Bean-nodulating Rhizobium etli originated in Mesoamerica, while soybean-nodulating Sinorhizobium fredii evolved East Asia. S. strains, such as GR64, have been isolated from bean nodules Spain, suggesting the occurrence of conjugative transfer events between introduced and native strains. In R. CFN42, symbiotic plasmid (pRet42d) requires cointegration with endogenous self-transmissible pRet42a. Aiming at further understanding generation diversity among nodulating we...
The bacterial genus Rhizobium comprises diverse symbiotic nitrogen-fixing species associated with the roots of plants in Leguminosae family. Multiple genomic clusters defined by whole genome comparisons occur within Rhizobium, but their equivalence to is controversial. In this study we investigated such ascertain significance a phylogeny context. Phylogenomic inferences based on complete sets ribosomal proteins and stringent core markers revealed main lineages Rhizobium. clades corresponding...