A5041760425- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Biosensors and Analytical Detection
- Helminth infection and control
- Glycosylation and Glycoproteins Research
- Streptococcal Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Clostridium difficile and Clostridium perfringens research
- Dengue and Mosquito Control Research
- Hepatitis Viruses Studies and Epidemiology
- Radiation Therapy and Dosimetry
- Essential Oils and Antimicrobial Activity
- Parasite Biology and Host Interactions
- Genomics and Phylogenetic Studies
- COVID-19 epidemiological studies
- Yersinia bacterium, plague, ectoparasites research
- Parasites and Host Interactions
Instituto de Salud Pública de Chile
2015-2024
Escuela Politécnica del Ejército
2019
The current pandemic caused by the new coronavirus is a worldwide public health concern. To aboard this emergency, and like never before, scientific groups around world have been working in fast coordinated way to get maximum of information about virus when it has almost 3 months since first cases were detected Wuhan province China. complete genome sequences 450 isolates are available, studies similarities differences among them with close related viruses that similar epidemics century. In...
Abstract Genomic surveillance (GS) programmes were crucial in identifying and quantifying the mutating patterns of SARS-CoV-2 during COVID-19 pandemic. In this work, we develop a Bayesian framework to quantify relative transmissibility different variants tailored for regions with limited GS. We use it study Chile. Among 3443 genomes collected between January June 2021, where sampling was designed be representative, Gamma (P.1), Lambda (C.37), Alpha (B.1.1.7), B.1.1.348, B.1.1 lineages...
Clostridium difficile infection has gained importance in recent years as a result of the rapid spread epidemic strains, including hypervirulent strains. This study reports molecular epidemiology C. obtained from hospitalized patients Chile. Seven hundred and nineteen isolates toxigenic 45 hospitals across country were characterized through toxin profile, pulsed-field gel electrophoresis (PFGE), sequencing tcdC gene. In addition, polymerase chain reaction (PCR) ribotyping multilocus sequence...
Vaccination generates a neutralizing immune response against SARS-CoV-2. The genomic surveillance is showing the emergence of variants with mutations in spike, main target antibodies. To understand impact these variants, we report neutralization potency alpha, gamma, and D614G SARS-CoV-2 44 individuals that received two doses CoronaVac vaccine, an inactivated vaccine. Plasma samples collected at 60 days after second dose were analyzed by reduction cytopathic effect Vero E6 cells three...
Zoonotic transmission of Salmonella infections causes an estimated 11% salmonellosis annually in the United States. This report describes epidemiologic, traceback and laboratory investigations conducted States as part four multistate outbreaks linked to small turtles. isolates indistinguishable from outbreak strains were isolated a total 143 ill people States, pet turtles, pond water samples collected turtle farm A, well Chile Luxembourg. Almost half (45%) occurred children aged <5 years,...
We report the first description of a rare catalase-negative strain Staphylococcus aureus in Chile. This new variant was isolated from blood and synovial tissue samples pediatric patient. Sequencing analysis revealed that this is related to ST10 strain, which has earlier been described relation S. carriers. Interestingly, sequence catalase gene katA presence novel nonsense mutation causes premature translational truncation C-terminus enzyme leading loss 222 amino acids. Our study suggests...
Salmonella spp. is a prevalent pathogen that causes great public health concern worldwide. Bacteriophage-based cocktails have arisen as an alternative to antibiotics inhibit the growth of Salmonella. However, bactericidal effect bacteriophage in vivo largely differs from their observed vitro. This partly because vitro developments do not always consider bacterial diversity nor environmental conditions where bacteriophages will replicate. Here, we isolated and sequenced 47 showed variable...
Objective: To elucidate whether the genetic platforms of blaCTX-M contribute to phenotypes multi-drug-resistance (MDR) in first carbapenemase-producing K. pneumoniae strains isolated Chile. Method: Twenty-two from different Chilean patients and hospitals were studied. Their relatedness was assessed by PFGE MLST. The levels antibiotic resistance evaluated determining minimum inhibitory concentration various antimicrobials. In addition, several genes clinical relevance Chile investigated....
The emergence of SARS-CoV-2 variants concern endangers the long-term control COVID-19, especially in countries with limited genomic surveillance. In this work, we explored drivers contagion Chile. We sequenced 3443 genomes collected between January and July 2021, where Gamma (P.1), Lambda (C.37), Alpha (B.1.1.7), B.1.1.348, B.1.1 lineages were predominant. Using a Bayesian model tailored for surveillance, found that variants' reproduction numbers about 5% 16% larger than Alpha's,...
Listeria monocytogenes is a foodborne pathogen that causes listeriosis, disease can present as febrile gastroenteritis or an invasive form has high mortality rates. So far, the genetic diversity of strains L. isolated from patients, foods and environmental sources in Chile been poorly studied.To characterize genetically received by Institute Public Health (ISP) between 2007 2014.We selected 94 corresponding to different pulsotypes identified pulsed field gel electrophoresis (PFGE), DNA was...
<title>Abstract</title> The use of bacteriophages for biological control bacterial infections is a promising approach to combat antimicrobial resistant bacteria. Prediction phage-bacteria interactions key identify sensitive strains phage therapy. Since these are governed by multiple mechanisms, it not simple task predict the outcome infection, which varies even among from same species. In this study, machine learning-based models capable predicting host range phages sequencing data were...
In recent studies, emphasis has been placed on the zonula occludens toxin (Zot) from non-toxigenic Vibrio parahaemolyticus strain PMC53.7 as an agent inducing alterations in actin cytoskeleton of infected Caco-2 cells and which appears a relevant virulence factor. Universal zot primers were designed by alignment different types gene identification conserved sequences to investigate presence diverse environmental clinical V. isolates, co-occurrence with factors, such hemolysins secretion...
Salmonella is a zoonotic pathogen transmitted through food and one of the four leading global causes diarrheal disease in humans. Brazil world's largest exporter poultry meat, as such, Brazilian farms routinely detect prevent Salmonella. The last official monitoring control slaughter establishments 2023 detected spp. 14.80 % samples from chickens, those Salmonella-positive were classified serovar Minnesota 65.76 samples, Heidelberg 16.10 another 26 or more serovars non-typeable represented...