A5011764279- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Agronomic Practices and Intercropping Systems
- Cassava research and cyanide
- Nematode management and characterization studies
- Coastal wetland ecosystem dynamics
- Polyamine Metabolism and Applications
- Soybean genetics and cultivation
- Plant responses to water stress
- Polymer-Based Agricultural Enhancements
- Proteins in Food Systems
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Pathogenic Bacteria Studies
- Phytase and its Applications
- Plant Parasitism and Resistance
- Agriculture, Soil, Plant Science
- Plant Growth Enhancement Techniques
Universidad de Granada
2006-2023
Estación Experimental del Zaidín
1997-2006
Consejo Superior de Investigaciones Científicas
2003-2006
Aarhus University
2006
Gene Therapy Laboratory
2006
Inoculation of alfalfa plants with either incompatible Rhizobium or a mutant blocked in Nod factor synthesis led to an accumulation salicylic acid roots, contrast inoculated wild-type (compatible) R. meliloti strain. When was exogenously applied prior inoculation purified compatible strains, significant inhibition nodule primordia formation and reduction the number emerging nodules, respectively, as well delay visualization, were observed. These results suggest involvement...
Trehalose (alpha-D-glucopyranosyl-1,1-alpha-D-glucopyranoside), a non-reducing disaccharide, has been found in wide variety of organisms playing an important role as abiotic stress protectant. Plants may come into contact with trehalose from exogenous sources, such plant-rhizobia symbiosis which the rhizobia have capacity to produce trehalose. The aim this work is analyse how and trehalase respond salt root nodules legumes. For purpose, tissue expression Medicago truncatula gene (MTTRE1)...
ABSTRACT An analysis of the conjugative transfer pRetCFN42d, symbiotic plasmid (pSym) Rhizobium etli , has revealed a novel gene, rctA as an essential element regulatory system for silencing R. pSym by repressing transcription conjugal genes in standard laboratory media. The gene product lacks sequence conservation with other proteins known function but may belong to winged-helix DNA-binding subfamily transcriptional regulators. Similar that many repressors, seems be positively...
The effect of NaCl stress (50 m M ) and validamycin A treatment (30 μ on growth nitrogen fixation Lotus japonicus was investigated in plants cultured under symbiotic hydroponics conditions for teen weeks (flowering stage). Validamycin used as a potent trehalase inhibitor, able to produce five‐fold increase the level trehalose during salt treatment, concomitant with an enhance biomass (20%) salinized plants. Alterations nodule metabolism related some carbohydrates enzyme activities were also...
An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, Olivares, and Sanjuán, Bacteriol. 180:4583-4590, 1998) was modified improve its reliability prevent selection undesired false mob clones. By following this approach, we were able two regions the Rhizobium etli CFN42. One corresponds recently characterized region nonsymbiotic, self-transmissible plasmid pRetCFN42a (C....
In recent decades, the quality of agricultural soils has been seriously affected by excessive application pesticides, with herbicides being one most abundant. Continuous use alters soil microbial community and beneficial interactions between plants bacteria such as legume-rhizobia spp. symbiosis, causing a decrease in biological nitrogen fixation, which is essential for fertility. Therefore, aim this work was to study effect two commonly used (pendimethalin clethodim) on symbiosis improve...
ABSTRACT A simple approach was used to identify Rhizobium meliloti DNA regions with the ability convert a nontransmissible vector into mobilizable plasmid, i.e., contain origins of conjugative transfer ( oriT , mob ). RecA-defective R. merodiploid populations, where each individual contained hybrid cosmid from an GR4 gene library, were as donors en masse in conjugation another recipient strain, selecting transconjugants for vector-encoded antibiotic resistance. Restriction analysis cosmids...
• The expression of nodA and dctA genes Rhizobium leguminosarum bv. viciae has been studied in mutant nodules pea (Pisum sativum L.), blocked at the following developmental stages: infection thread development inside nodule (Itn); droplet differentiation (Idd); bacteroid after endocytosis (Bad); persistence (Nop). With use reporter fusions to these symbiotic bacterial it was shown that both were expressed all stages, with a pattern similar constitutive, symbiosis-unrelated genes. As well as...
Genetic and biochemical characterization of TraA, the relaxase symbiotic plasmid pRetCFN42d from Rhizobium etli, is described. After purifying domain (N265TraA), we demonstrated nic binding cleavage activity in vitro thus characterized for first time nick site (nic) a family Rhizobiaceae. We studied range N265TraA specificity by testing different oligonucleotides nicking assays. In addition, ability to mobilize Rhizobiaceae origins transfer (oriT) was examined. Data obtained with these...
We have examined nutritional factors influencing conjugal transfer of the two nonsymbiotic large plasmids, pRmeGR4a and pRmeGR4b, Rhizobium meliloti GR4. To monitor transfer, each plasmid was tagged with a different antibiotic resistance marker. Transfer pRmeGR4b dependent upon presence on same donor cell. Transconjugants for were obtained at frequencies 5-to 10-fold higher than transconjugants carrying both indicating that mobilization by probably occurred in trans. Conjugal plasmids...
In this study, we examine how indeterminate and determined nitrogen-fixing root nodules of model legumes Lotus japonicus Medicago truncatula adapt their non-structural carbohydrate pool during salt stress, with particular emphasis on trehalose, a compatible solute abundant in some legumes. M. L. plants were inoculated Sinorhizobium meliloti Mesorhizobium loti, respectively, the effect 50 mM sodium chloride (NaCl) added to nutrient solution was studied time-course experiment. Sucrose pinitol...
Rhizobium meliloti recA strains have been reported as safe hosts for deliberate release experiments due to their reduced survival in soil microcosms. Using R. integration vectors, two GUS-positive RecA+ and RecA− isogenic derivatives of strain GR4 were constructed. In contrast previously strains, the GR4KLR showed similar doubling times its GR4KLC. Accordingly, normal growth competition capacity when coinoculated with parental sterile addition, mutant delayed nodulation on axenically grown...
The genetic relationships among bacteria nodulating Phaseolus vulgaris in a soil of Granada, Spain, were investigated by multilocus enzyme electrophoresis, restriction fragment length polymorphism and partial sequencing the 16S rRNA genes polymorphisms symbiotic genes. Multilocus electrophoresis analysis 39 isolates determined 11 different electrophoretic types, clustered into three main groups. Genetic distances between groups above 0.8. Five gene alleles identified this population,...