A5052342645- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Mesenchymal stem cell research
- Cytomegalovirus and herpesvirus research
- Hematopoietic Stem Cell Transplantation
- NF-κB Signaling Pathways
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Protein Degradation and Inhibitors
- HIV Research and Treatment
- Extracellular vesicles in disease
- Retinoids in leukemia and cellular processes
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Bone and Joint Diseases
- RNA regulation and disease
- Chemokine receptors and signaling
- Blood disorders and treatments
- Molecular Biology Techniques and Applications
- Ferroptosis and cancer prognosis
- Lymphatic System and Diseases
- Endoplasmic Reticulum Stress and Disease
- Cytokine Signaling Pathways and Interactions
Gamida Cell (Israel)
2021
University of California, San Diego
2017
Harvard University Press
2002
Thomas Jefferson University
2002
Boston University
1997-1999
Pulmonary Associates
1998
Engineered regulatory T (T reg ) cells have emerged as precision therapeutics aimed at inducing immune tolerance while reducing the risks associated with generalized immunosuppression. This Viewpoint highlights opportunities and challenges for engineered cell therapies in treating autoimmune other inflammatory diseases.
CCAAT/enhancer binding protein α (C/EBPα) is an integral factor in the granulocytic developmental pathway, as myeloblasts from C/EBPα-null mice exhibit early block differentiation. Since deficient for known C/EBPα target genes do not same granulocyte maturation, we sought to identify additional essential myeloid cell development. To such genes, used both representational difference analysis and oligonucleotide array with RNA derived a C/EBPα-inducible line. From each of these independent...
Adult human bone marrow-derived stem cells, having the ability to differentiate into cells of multiple lineages, have been isolated and propagated by varied protocols, including positive (CD105(+))/negative (CD45(-)GlyA(-)) selection with immunomagnetic beads, or direct plating selective culture media. Each substratum-adherent cell population was subjected a systematic analysis their surface markers differentiation potential. In initial stages culture, each proliferated slowly, reaching...
Mesenchymal stem cell (MSC) therapy has shown promise clinically in graft-versus-host disease and preclinical animal models of T helper type 1 (Th1)-driven autoimmune diseases, but whether MSCs can be used to treat general is unclear. Here, the therapeutic potential was tested New Zealand black (NZB)xNew white (NZW) F1 (NZB/W) lupus mouse model. The pathogenesis systemic erythematosus involves abnormal B activation leading autoantibody formation. To test immunomodulatory activity would...
We previously reported that LPS stimulation of the RAW264.7 murine macrophage cell line rapidly induced a structural change within N terminus transcriptional regulatory factor PU.1. PU.1 is required for expression variety cytokine, cytokine receptor, and integrin genes. Western blot analysis nuclear extracts prepared from LPS-stimulated macrophages revealed increased phosphorylation at serine residues relative to in unstimulated controls. PU.1-DNA complexes using were less sensitive protease...
The human secretory interleukin-1 receptor antagonist (secretory IL-1Ra) gene is controlled through three lipopolysaccharide (LPS)-responsive promoter elements, one of which was identified as an NF-kappaB binding site. Sequence analysis the IL-1Ra a potential PU.1 site located between positions -80 and -90 on complementary strand overlapping Gel shift using this with nuclear extracts from RAW 264.7 macrophages demonstrated formation complexes, LPS-inducible two constitutive. inducible factor...
Abstract We recently reported that LPS stimulation of monocytic cells leads to the activation PU.1, a member Ets family transcription factors. Phosphorylation PU.1 by protein kinase CK2 was found up-regulate its trans-activation function, but not DNA binding activity. Previous studies suggested proteins could bind NF-κB motifs at tetrameric core sequence TTCC. In macrophages, LPS-inducible HIV-1 gene expression is mediated in part identical tandem sites located within long terminal repeat...
Abstract Despite advances in Type 1 Diabetes (T1D) management such a hybrid closed loop systems, patients still face significant morbidity, reduced life expectancy, and impaired glucose regulation compared to healthy individuals or those with pancreas transplants. Here we developed beta cell-specific Chimeric Antigen Receptors (CAR) targeting the antigen ectonucleoside triphosphate diphosphohydrolase 3 (ENTPD3) using novel cell-based phage display methodology. ENTPD3 is highly expressed on...
Stimulation of macrophages by Gram-negative bacterial lipopolysaccharide (LPS) rapidly leads to the activation several protein kinases and subsequent phosphorylation transcription factors that regulate LPS-inducible genes. Several investigators have shown MAP (MAPKs) are activated following LPS stimulation. We previously reported stimulation also up-regulates enzymatic activity kinase CK2, a ubiquitous serine/threonine kinase, although signaling cascade CK2 in is unknown. Because MAPKs known...
<h3>Background</h3> Adoptive transfer of Natural Killer cells (NKs) is a growing area innovation in cancer immunotherapy. Nicotinamide (NAM), an allosteric inhibitor NAD-dependent enzymes, has been shown to preserve cell function and prevent differentiation ex vivo culture NK (NAM-NK) other cells. Clinical responses were observed Phase 1 trial NAM-NK (GDA-201) patients with refractory non-Hodgkin lymphoma (Bachanova, et. al., Blood 134:777, 2019). We now characterize the mechanisms...
Abstract CD38 is a cell surface protein expressed primarily on white blood cells and considered marker of differentiation initiation. involved in the immune system by engaging cross-talk with T B as well activation NK cells. In multiple myeloma (MM), subset tumor have high expression (CD38hi), which has led to development effective anti-CD38 therapeutic antibodies, such daratumumab (DARA). Thus, cancer that express can be selectively targeted for elimination using these antibodies. myeloma,...
Historical efforts at expansion of umbilical cord blood (UCB) derived CD34+ hematopoietic stem cells (HSCs) ex vivo with cytokines yielded large numbers progenitors for transplantation but impaired their long-term engraftment. We used nicotinamide (NAM), an allosteric inhibitor NAD-enzymes, to create omidubicel, investigational cell therapy designed improve the HSCs bone marrow transplant. A Phase 1/2 clinical study in patients high-risk hematologic malignancies showed rapid neutrophil...