A5064201870- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Barrier Structure and Function Studies
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- CAR-T cell therapy research
- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Dementia and Cognitive Impairment Research
- Virus-based gene therapy research
- Signaling Pathways in Disease
- Caveolin-1 and cellular processes
- Alzheimer's disease research and treatments
- Neurological Disease Mechanisms and Treatments
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
- Glioma Diagnosis and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Neurological disorders and treatments
- CRISPR and Genetic Engineering
- Prion Diseases and Protein Misfolding
- Drug Transport and Resistance Mechanisms
AstraZeneca (Sweden)
2017-2024
University of Skövde
2017-2020
University of Gothenburg
2017-2020
AstraZeneca (United States)
2017
Tufts University
2017
Abstract Cell-based models of the blood–brain barrier (BBB) are important for increasing knowledge BBB formation, degradation and brain exposure drug substances. Human preferred over animal because interspecies differences in structure function. However, access to human primary tissue is limited has shown degeneration functions vitro. induced pluripotent stem cells (iPSCs) can be used generate relevant cell types model with tissue. We generated a iPSC-derived that includes endothelial...
In vivo studies of human brain cellular function face challenging ethical and practical difficulties. Animal models are typically used but display distinct differences. One specific example is astrocytes, recently recognized for contribution to neurological diseases a link the genetic risk factor apolipoprotein E (APOE). Current astrocytic in vitro questioned lack biological characterization. Here, we report induced pluripotent stem cell (hiPSC)-derived astroglia (NES-Astro) developed under...
Mutations in the gene TARDBP, which encodes TAR DNA-binding protein 43 (TDP-43), are a rare cause of familial forms amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). While majority mutations found C-terminal glycine-rich domain, an alanine to valine amino acid change at position 90 (A90V) bipartite nuclear localization signal TDP-43 has been described. This sequence variant previously shown cytoplasmic mislocalization decreases solubility, leading formation insoluble...
The RNA-binding and -processing protein TAR DNA-binding 43 (TDP-43) is heavily linked to the underlying causes pathology of neurodegenerative diseases such as amyotrophic lateral sclerosis frontotemporal lobar degeneration. In these diseases, TDP-43 mislocalized, hyperphosphorylated, ubiquitinated, aggregated cleaved. importance cleavage in disease pathogenesis still poorly understood. Here we detail use D-sorbitol an exogenous stressor that HeLa cells, resulting a 35 kDa truncated product...
Aim & methods: The Health and Environmental Sciences Institute Cell Therapy-TRAcking, Circulation Safety Technical Committee launched an international, multisite study to evaluate the sensitivity reproducibility of highly efficient culture (HEC) assay, in vitro assay detect residual undifferentiated human pluripotent stem cells (hPSCs) cell therapy products. Results: All facilities detected colonies induced (hiPSCs) when five hiPSCs were spiked into 1 million hiPSC-derived cardiomyocytes....
Human induced pluripotent stem cells (hiPSC) hold great promise for use in cell therapy applications and improved vitro models of human disease. So far, most hiPSC differentiation protocols to astroglia undefined, animal-containing culture matrices. Laminins, which play an essential role the regulation behavior, offer a source defined, animal-free matrix. In order understand how laminins affect differentiation, recombinant laminin-521 (LN521), was compared murine Engelbreth-Holm-Swarm...
Abstract Despite advances in Type 1 Diabetes (T1D) management such a hybrid closed loop systems, patients still face significant morbidity, reduced life expectancy, and impaired glucose regulation compared to healthy individuals or those with pancreas transplants. Here we developed beta cell-specific Chimeric Antigen Receptors (CAR) targeting the antigen ectonucleoside triphosphate diphosphohydrolase 3 (ENTPD3) using novel cell-based phage display methodology. ENTPD3 is highly expressed on...